The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characteristics of Cucumber mosaic virus Infecting Zucchini in Korea

  • Kim, Mi-Kyeong (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Kwak, Hae-Ryun (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Jeong, Seon-Gi (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Ko, Sug-Ju (Jeonnam Agricultural Research and Extension Services) ;
  • Lee, Su-Heon (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Kim, Jeong-Soo (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University) ;
  • Choi, Jang-Kyung (Department of Agricultural Biology, Kangwon National University) ;
  • Choi, Hong-Soo (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
  • Received : 2010.01.18
  • Accepted : 2010.05.18
  • Published : 2010.06.30
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Abstract

A virus causing stunt, yellowing, severe mosaic, malformation symptoms on leaves and uneven development and malformation on fruits of zucchini was prevalent around Goseong, Gyeongsangnam-do, Korea. A survey conducted (2004) in the Goseong area revealed about 20% virus infection rate. The disease causative identified as Cucumber mosaic virus (CMV-Z1) was further characterized. The isolate induces mosaic symptoms on Cucumis sativus, while severe mosaic, stunt and malformation on C. pepo. Thin section analyses have shown that virus inclusions are formed in the cuticle layers as well as epidermal, parenchyma and collenchymas cells in virus-infected Nicotiana tabacum. CMV-Z1 isolate induced specific cytoplasmic inclusion bodies such as irregular clumps (IC), crystal (Cr) and irregular chloroplasts (ICh). IC was made up of virus particles interspersed with a darkly stained amorphous material and found both in the cytoplasm and vacuoles, whereas ICh and Cr were rarely found in the vacuoles. The genome of CMV-Z1 RNA-1 consists of 3359 nucleotide (nt) encoding 1a protein of 993 amino acids (aa). The CMV-Z1 RNA-2 was 3050 nt in length containing 2a (857 aa) and 2b (110 aa), while RNA-3 encoding 3a movement protein (279 aa) and coat protein (218 aa) was 2215 nt in length. Phylogenetic analyses of nucleotide sequences of CMV-Z1 isolate appeared it is more closely related to subgroup IA than to subgroup IB or II.

Keywords

References

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