Advances in Traditional Medicine

  • 제10권3호
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  • Pages.155-164
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  • 2010
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  • 2662-4052(pISSN)
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  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
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Aqueous extract of Lycii fructus suppresses inflammation through the inhibition of nuclear factor kappa B signal pathway in murine raw 264.7 macrophages

  • Kim, Beum-Seuk (Department of Rehabilitation Medicine, College of Oriental Medicine, Kyung Won University) ;
  • Lim, Hyung-Ho (Department of Rehabilitation Medicine, College of Oriental Medicine, Kyung Won University) ;
  • Song, Yun-Kyung (Department of Rehabilitation Medicine, College of Oriental Medicine, Kyung Won University) ;
  • Sung, Yun-Hee (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Kim, Sung-Eun (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Chang, Hyun-Kyung (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Shin, Mal-Soon (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Kim, Chang-Ju (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Lee, Hye-Jung (Acupuncture and Meridian Science Research Center, Kyung Hee University) ;
  • Kim, Dong-Hee (Department of Ophthalmology, College of Medicine, Chungju Hospital, Konkuk University)
  • 투고 : 2010.08.24
  • 심사 : 2010.09.07
  • 발행 : 2010.09.30
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초록

Lycii fructus is the fruit of Lycium chinense Miller and is part of the Solanaceae family. Lycii fructus produces various effects such as hypotensive, hypoglycemic, anti-pyretic, and anti-stress activities. Lycii fructus is known to contain betaine, carotene, nicotinic acid, zeaxanthin, and cerebroside. In the present study, the effects of Lycii fructus aqueous extract on lipopolysaccharide (LPS)-induced inflammation in murine raw 264.7 macrophage cells were investigated. In this study we utilized the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcriptionpolymerase chain reaction (RT-PCR), Western blotting, and nitric oxide (NO) detection. Lycii fructus aqueous extract suppressed NO production by inhibiting the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-$\alpha$) mRNA and iNOS protein in murine raw 264.7 macrophage cells. Also, Lycii fructus aqueous extract suppressed the activation of nuclear factor-kappa B (NF-${\kappa}B$) in the nucleus. These results demonstrated that Lycii fructus aqueous extract causes an anti-inflammatory effect that was likely produced by the suppression of iNOS expression through the down-regulation of NF-$\hat{e}B$ binding activity.

키워드

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