The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Hepatoprotective Effect of Gagam-GongJin-dan extract against Acetaminophen-Induced Liver Injury in Mice

Acetaminophen 유도 마우스 간 손상에 대한 가감공진단(加減拱辰丹) 추출물의 간보호 효과

  • Kim, Hong-Hun (Department of Oriental Medical Prescription, Woosuk University) ;
  • Mok, Ji-Ye (Jeonju University Atopy & Health Research Institute) ;
  • Park, Kwang-Hyun (Department of Biochemistry, Chonbuk National University Medical School) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Hwang, Byung-Soon (Jeonju Biomaterials Institute) ;
  • Hwang, Sung-Yeoun (Department of Oriental Medical Prescription, Woosuk University) ;
  • Cho, Jung-Keun (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Jang, Seon-Il (Jeonju University Atopy & Health Research Institute)
  • 김홍준 (우석대학교 한의과대학 본초방제학교실) ;
  • 목지예 (전주대학교 아토피.건강전문연구소) ;
  • 박광현 (전북대학교 의약전문대학원 생화학교실) ;
  • 정승일 (전주생물소재연구소) ;
  • 황병순 (전주생물소재연구소) ;
  • 황성연 (우석대학교 한의과대학 본초방제학교실) ;
  • 조정근 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 장선일 (전주대학교 아토피.건강전문연구소)
  • Received : 2010.08.15
  • Accepted : 2010.09.13
  • Published : 2010.09.30
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Abstract

Objective：Gagam-Gongjin-dan (GGD) is an oriental medicinal prescription composited with Cervi parvum Cornu, Corni Fructus, Angelica Gigantis Radix, Lycii Fructus, Dioscoreae Rhizoma, Citri Pericarpium, Gastrodiae Rihzoma, Agastachis Herba, Cassiae cortex, Scutellariae Radix and Schisandrae Fructus. The purpose of this study was to investigate the effects of GGD extract against acetaminophen (APAP)-induced liver injury in mice. Methods：GGD extract was prepared by extracting with methanol for 7 days. The extract was freeze-dried following filtration through vacuum distillation system. The first, we investigated the antioxidant effects of GGD extract on electronic donating ability (DPPH), nitrite (NO) scavenging and superoxide dismutase (SOD)-like activity. The next, we investigated the possible hepatoprotective effect of GGD extract administration against acetaminophen-induced liver injury in mice. Mice were orally administrated with or without GGD extract of different doses (25-100 mg/kg/day) one times per day for 6 days. After 3 days, APAP was orally applied with a single dose (400 mg/kg). Results：GGD extract increased DPPH, NO and SOD-like activities in dose dependant. APAP treatment significantly increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in plasma. Also, APAP treatment significantly evaluated lipid peroxidation product thiobarbituric reacting substances (TBARS) and depleted some antioxidant enzymes (superoxide dismutase, catalase, d-aminolevulinate dehydratase and gluthathione peroxidase activities) in liver homogenates compared to the control group. However, the orally administration of GGD extract was able to counteract these effects. Histological studies provided supportive evidence for biochemical analysis Conclusions：These results suggest that GGD extract has a potential antioxidant and hepatoprotective effect against APAP-induced liver injury, these properties may contribute to liver disease care.

Keywords

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