Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
권호 표지

Biodisc Regeneration Using Annulus Fibrosus Cell with Hyaluronic Acid Impregnated Small Intestinal Submucosa Sponge

히알루론산이 함유된 SIS 스폰지와 섬유륜세포를 이용한 디스크재생

  • Hong, Hee-Kyung (Department of BIN Fusion Technology & Department of Polymer Nano Science & Technology, Chonbuk National University) ;
  • Lee, Seon-Kyoung (Department of BIN Fusion Technology & Department of Polymer Nano Science & Technology, Chonbuk National University) ;
  • Song, Yi-Seul (Department of BIN Fusion Technology & Department of Polymer Nano Science & Technology, Chonbuk National University) ;
  • Kim, Dae-Sung (Department of BIN Fusion Technology & Department of Polymer Nano Science & Technology, Chonbuk National University) ;
  • Eom, Shin (Department of BIN Fusion Technology & Department of Polymer Nano Science & Technology, Chonbuk National University) ;
  • Kim, Hyoung-Eun (Department of BIN Fusion Technology & Department of Polymer Nano Science & Technology, Chonbuk National University) ;
  • Lee, Dong-Won (Department of BIN Fusion Technology & Department of Polymer Nano Science & Technology, Chonbuk National University) ;
  • Khang, Gil-Son (Department of BIN Fusion Technology & Department of Polymer Nano Science & Technology, Chonbuk National University)
  • 홍희경 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 이선경 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 송이슬 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 김대성 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 엄신 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 김형은 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 이동원 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 강길선 (전북대학교 BIN 융합공학과, 고분자나노공학과)
  • Received : 2010.01.20
  • Accepted : 2010.02.10
  • Published : 2010.05.25
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Abstract

The porcine small intestinal submucosa (SIS) has been widely used as a biomaterial without immuno rejection responses and hyalunonic acid (HA) can be used as biocompatible materials to regenerate tissue. We developed the SIS sponge and HA loaded SIS sponges (SIS/HA) for the possibility of the application of the tissue engineering using annulus fibrosus (AF). SEM observation shows that SIS and SIS/HA sponges have interconnected and open pores. We demonstrated the presence of HA in SIS/HA sponge from C-O functional group observed by the FTIR analysis. In conclusion, we suggest that SIS/HA sponge may be useful to tissue engineering using AF cell. This may be due to the enhanced biocompatibility and higher water retention capacity of HA.

소장점막하조직(SIS)은 면역반응이 없어 생체물질로 널리 사용되고 있다. 히알루론산(HA)은 천연 보습제라 불릴 정도로 친수성이 매우 뛰어나고 조직을 수복하기 위한 체내이식용 구조물로써 널리 사용되고 있는 천연고분자이다. 본 연구에서는 섬유륜세포를 이용한 조직공학 디스크 재생을 위해 HA를 함유한 SIS/HA 스폰지를 제조하였다. 제조된 스폰지 내부의 전자현미경 관찰 결과 열린 다공구조를 하고 있음을 확인하였고, FTIR 분석을 통해 C-O 관능기가 관찰 되어 SIS/HA 스폰지 내부의 HA가 빠져나가지 않고 고르게 분포되어 있음을 확인하였다. 결론적으로 SIS/HA 스폰지는 SIS와 HA 각각의 특성을 유지하며 섬유륜세포의 성장에 유익한 환경을 제공함을 확인하였고, 이는 HA의 우수한 생체적합성 및 수분 보유능력에 기인된 것으로 사료된다.

Keywords

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