Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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DETECTION OF SALIVARY STREPTOCOCCUS MUTANS LEVELS USING MONOCLONAL ANTIBODIES

단클론항체를 이용한 타액 내 Streptococcus mutans 수준의 측정

  • Kim, Chu-Sung (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Kim, Jae-Gon (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Yang, Yeon-Mi (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Baik, Byeong-Ju (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Lee, Kyung-Yeol (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Kim, Mi-Ah (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Lim, Su-Min (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
  • 김추성 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 김재곤 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 양연미 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 백병주 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 이경열 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 김미아 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 임수민 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소)
  • Received : 2010.02.17
  • Accepted : 2010.05.07
  • Published : 2010.05.31
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Abstract

Streptococcus mutans, one of the major causal agents of dental caries, is component of the dental plaque. It produces various organic acids such as lactic acid which is the end-product of glycolysis, and this leads to dental caries. A new system using species-specific monoclonal antibodies was developed to detect Streptococcus mutans in saliva. The system quickly detects salivary Streptococcus mutans in 30min and classifies the result into two levels. The purpose of this study was to investigate correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods. Children's deft indices were also compared with Streptococcus mutans counts in MSB agar plate. Subjects consisted of 15 children in the age of 3 to 6 years. They were assigned to three groups : Group I(deft index = 3), Group II(deft index $\leqq$3), Group III(deft index $\geqq$4). The results are as follows : 1. The rate of children with positive response was 13.3% and with negative response was 86.7% in the result of Saliva-checkTM Mutans test kit. 2. There was a positive correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods(p<0.05). 3. Streptococcus mutans counts in MSB agar plate were irrelevant to deft of children(p=0.34).

Streptococcus mutans는 구강 내에 상존하는 치아우식증의 주요 원인균으로서 치면의 피막에 부착 후 glucan을 형성하여 세균의 군락을 이루며, 외부로부터 공급된 자당대사를 통하여 유기산을 생성함으로써 법랑질을 탈회시킨다. 치아우식 활성도의 평가를 위한 단클론항체를 이용한 방법은 진료실에서 빠른 시간 내에 간편하게 타액에 존재하는 Streptococcus mutans의 정량분석이 가능한 방법이다. 이 연구는 3세에서 6세 사이의 어린이 15명을 대상으로 자극성 타액을 채취하여 시판 중인 단클론항체를 이용한 Salivacheck$^{TM}$ Mutans, strip을 이용한 Dentocult$^{(R)}$-SM 그리고 MSB배지 배양법으로서 타액 내 Streptococcus mutans를 측정한 후 그 값을 우식경험치아수와 비교하여 상관관계를 알아보았다. Saliva-check$^{TM}$ Mutans를 이용한 방법은 Dentocult$^{(R)}$-SM과 MSB배지법과 통계학적으로 유의한 상관관계를 보였으나(p<0.05), MSB배지법은 어린이의 우식경험치아수와 통계학적으로 유의한 결과를 나타내지 않았다(p=0.34).

Keywords

References

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