The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Loss of hsp70.1 Decreases Functional Motor Recovery after Spinal Cord Injury in Mice

  • Kim, Hyun-Jeong (Department of Dental Anesthesiology and Dental Research Institute, Seoul National University School of Dentistry) ;
  • Jung, Ji-In (Department of Physiology, Korea University College of Medicine) ;
  • Kim, Young-Kyung (Department of Physiology, Korea University College of Medicine) ;
  • Lee, Jae-Seon (Divisions of Radiation Cancer Research and Korea Institute of Radiological and Medical Sciences) ;
  • Yoon, Young-Wook (Department of Physiology, Korea University College of Medicine) ;
  • Kim, June-Sun (Department of Physical Therapy, Korea University College of Health Science)
  • Received : 2010.05.06
  • Accepted : 2010.05.19
  • Published : 2010.06.30
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Abstract

Heat shock proteins (HSPs) are specifically induced by various forms of stress. Hsp70.1, a member of the hsp70 family is known to play an important role in cytoprotection from stressful insults. However, the functional role of Hsp70 in motor function after spinal cord injury (SCI) is still unclear. To study the role of hsp70.1 in motor recovery following SCI, we assessed locomotor function in hsp70.1 knockout (KO) mice and their wild-type (WT) mice via the Basso, Beattie and Bresnahan (BBB) locomotor rating scale, before and after spinal hemisection at T13 level. We also examined lesion size in the spinal cord using Luxol fast blue/cresyl violet staining. One day after injury, KO and WT mice showed no significant difference in the motor function due to complete paralysis following spinal hemisection. However, when it compared to WT mice, KO mice had significantly delayed and decreased functional outcomes from 4 days up to 21 days after SCI. KO mice also showed significantly greater lesion size in the spinal cord than WT mice showed at 21 days after spinal hemisection. These results suggest that Hsp70 has a protective effect against traumatic SCI and the manipulation of the hsp70.1 gene may help improve the recovery of motor function, thereby enhancing neuroprotection after SCI.

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References

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