Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Investigation of Norovirus Occurrence and Influence of Environmental Factors in Food Service Institutions of ChungCheong Area

충청지역 집단급식소의 노로바이러스 실태조사와 환경요인의 영향

  • Jung, Woo-Young (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Eom, Joon-Ho (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Kim, Byeong-Jo (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Yun, Min-Ho (Bio Environmental Chemistry, Chungnam National University) ;
  • Ju, In-Sun (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Kim, Chang-Soo (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Kim, Mi-Ra (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Byun, Jung-A (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Park, You-Gyoung (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Son, Sang-Hyuck (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Lee, Eun-Mi (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Jung, Rae-Seok (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Na, Mi-Ae (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Yuk, Dong-Yeon (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Gang, Ji-Yeon (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration) ;
  • Heo, Ok-Sun (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
  • 정우영 (대전지방식품의약품안전청 유해물질분석과) ;
  • 엄준호 (대전지방식품의약품안전청 유해물질분석과) ;
  • 김병조 (대전지방식품의약품안전청 유해물질분석과) ;
  • 윤민호 (충남대학교 생물환경화학과) ;
  • 주인선 (대전지방식품의약품안전청 유해물질분석과) ;
  • 김창수 (대전지방식품의약품안전청 유해물질분석과) ;
  • 김미라 (대전지방식품의약품안전청 유해물질분석과) ;
  • 변정아 (대전지방식품의약품안전청 유해물질분석과) ;
  • 박유경 (대전지방식품의약품안전청 유해물질분석과) ;
  • 손상혁 (대전지방식품의약품안전청 유해물질분석과) ;
  • 이은미 (대전지방식품의약품안전청 유해물질분석과) ;
  • 정래석 (대전지방식품의약품안전청 유해물질분석과) ;
  • 나미애 (대전지방식품의약품안전청 유해물질분석과) ;
  • 육동연 (대전지방식품의약품안전청 유해물질분석과) ;
  • 강지연 (대전지방식품의약품안전청 유해물질분석과) ;
  • 허옥순 (대전지방식품의약품안전청 유해물질분석과)
  • Received : 2010.01.28
  • Accepted : 2010.05.10
  • Published : 2010.06.30
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Abstract

The purpose of this study was to examine the appearance of norovirus in the water for food in food service institutions and the influence of physicochemical and microbial factors of norovirus in order to work out basic data to predict the detection of norovirus. Among 82 samples of water for food in food service institutions, norovirus appeared in 7 samples and the rate of appearance was 8.5%. As for the type of norovirus, one samples contained GI type (genotype GI-6) and six samples contained GII type (genotype GII-2, GII-4, GII-12). In the regression model of prediction of norovirus, the rate of appearance was correlated with $NH_3$-N, total solids and the consumption of $KMnO_4$, out of such variables as $NH_3$-N, total solids, the consumption of $KMnO_4$, depth, chloride and total colony counts, and its contribution rate for effectiveness was 78.60%. In order to examine the influential factor of environment upon the detection of norovirus, Pearson's correlation analysis was carried out. The predictable regression formula for appearance rate of norovirus was expressed as -1.818 + 42.677 [$NH_3$-N] + 0.023 [total solids] + 0.762 [consumption of $KMnO_4$] -0.009 [depth] -0.146 [chloride] + 0.007 [total colony counts] (R = 0.904, $R^2$ = 0.818, adjusted $R^2$ = 0.786, p < 0.05). The most influential factors upon the detection of norovirus were $NH_3$-N, total solids and the consumption of $KMnO_4$. In other words, when the measured values of $NH_3$-N, total solids and the consumption of $KMnO_4$ were higher, the possibility of appearance of norovirus increased.

충청지역권내 지하수를 사용하는 집단급식소의 식품용수 82시료의 norovirus 검출빈도를 조사한 결과 7시료에서 검출 되었으며, 이는 8.5%에 해당 하였다. Norovirus가 검출된 집단 급식소에 대해서는 식중독 발생이 우려됨에 따라 소독, 관정개발, 오염원제거 및 상수도 보급 등의 위생환경 개선과 유지관리가 필요하다. Norovirus의 검출에 영향을 주는 요인으로는 암모니아성질소, 총고형분, 과망간산칼륨 소비량으로 나타났다. Norovirus 양성 검출군과 음성 검출군의 수질특성을 비교한 결과 수소이온농도는 큰 차이가 없었고, 탁도, 암모니아성질소, 일반세균수, 총고형분 항목에 있어서 norovirus 양성 검출군 평균값이 음성 검출군 평균값 보다 높은 값을 나타내었다. Norovirus 검출과 관련성이 높은 환경인자의 지속적인 모니터링을 통해 집단 급식소 식중독예방에 기여할 수 있을 것으로 기대된다.

Keywords

References

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