Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Genetic Relationship between SCCmec Types and Virulence Factors of Methicillin-Resistant Staphylococcus aureus Clinical Isolates in Korea

  • Lim, Kwan-Hun (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Lee, Gyu-Sang (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Park, Min (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Lee, Jin-Hee (Department of Clinical Laboratory Science, Kang-won National University Hospital) ;
  • Suh, In-Bum (Department of Clinical Laboratory Science, Kang-won National University Hospital) ;
  • Ryu, Sook-Won (Department of Clinical Laboratory Science, Kang-won National University Hospital) ;
  • Eom, Yong-Bin (Department of Biomedical Laboratory Science, Korea Nazarene University) ;
  • Kim, Jong-Bae (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University)
  • Received : 2010.06.04
  • Accepted : 2010.06.21
  • Published : 2010.06.30
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Abstract

The molecular epidemiological characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates have demonstrated their genetic diversity and evolution. A total of 137 strains of MRSA clinical isolates was collected from Korean healthcare facility in 2007. The MRSA clinical isolates were analyzed by molecular typings (SCCmec element and agr locus typing), virule nce factor gene detections {(Panton-Valentine leukocidin (PVL), enterotoxin, exfoliative toxin and toxic shock syndrome toxin-1), and amplified fragment length polymorphism (AFLP)}. The MRSA clinical isolates were classified as SCCmec type II-agr type 1 (2 strains), type II-agr type 2 (79 strains), type III-agr type 1 (24 strains), type III-agr type 2 (2 strains), type IV-agr type 1 (27 strains), type IV-agr type 2 (2 strains), and non-typable (1 strain, agr type 3). Based on SCCmec types, SCCmec type II (95.1%) and III (88.5%) indicated higher multidrug resistance rate than SCCmec type IV (10.3%) (P<0.001). The most common enterotoxin genes were seg (83.8%), sei (83.1%), and sec (80.2%). The tst gene was present in 86 out of 137 (62.8%) MRSA isolates. All MRSA isolates were negative for PVL and exfoliative toxin genes. The combinations of toxin genes were observed in particular SCCmec types; 97.6% of SCCmec type II strains carried sec, seg, sei and tst genes, 73.0% of SCCmec type III strains carried sea gene, and 89.7% of SCCmec type IV strains carried sec, seg and sei genes. Each of the SCCmec types of MRSA isolates had distinct AFLP profile. In conclusion, SCCmec type II, agr type 1 and 2 have demonstrated to be the most common types in Korea, and the results indicated that the virulence factors are closely associated with their molecular types (SCCmec and agr types).

Keywords

References

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