Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Association of -867G>C, -877Gdel, and Exon 5G>T Polymorphisms in the Stearoyl-CoA Desaturase (SCD) Gene with Fatty Acid Composition in the M. longissimus dorsi Muscle of Hanwoo (Korean Cattle)

  • Cho, Yong-Min (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Lee, Seung-Hwan (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Park, Eung-Woo (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Kim, Nam-Kuk (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Lim, Da-Jeong (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Kim, Kyoung-Hoon (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Park, Beom-Young (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Lee, Chang-Soo (Department of Applied Biochemistry, Kon-Kuk University) ;
  • Oh, Sung-Jong (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Kim, Tae-Hun (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Yoon, Du-Hak (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA)
  • Received : 2010.07.02
  • Accepted : 2010.07.27
  • Published : 2010.08.31
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Abstract

This study aimed to identify genetic polymorphisms associated with fatty acid composition in Hanwoo beef. In this study, three SNPs (-867G>C, -877Gdel and 878T>C) were detected in SCD gene by DNA sequencing and PCR-RFLP. Statistical analysis revealed that 878T>C SNP was significantly associated with total saturated (p=0.016), unsaturated (p=0.016), and monounsaturated fatty acid (p=0.026) composition. However, the other two SNPs (-867G>C and -877Gdel) that are detected in the regulatory region of the SCD gene have no association with the fatty acid composition of Hanwoo meat. The 878C (alanine type) allele was found to be associated with 2.2% higher monounsaturated fatty acid, 1.5% lower saturated fatty acid, and 1.4% higher unsaturated fatty acid content than those associated with the 878T (valine type) allele. These results indicate that the non-synonymous SNP (878T>C) in the SCD gene could be a causal mutation that contributes to the MUFA variation in Hanwoo beef.

Keywords

References

  1. Chung, K., Lunt, D., Kawachi, H., Yano, H., and Smith, S. (2007) Lipogenesis and stearoyl-CoA desaturase gene expression and enzyme activity in adipose tissue of short-and long-fed Angus and Wagyu steers fed corn-or hay-based diets. J. Anim. Sci. 85, 380-387. https://doi.org/10.2527/jas.2006-087
  2. Clemens, E., Arthaud, V., Mandigo, R., and Woods, W. (1973) Fatty acid composition of bulls and steers as influenced by age and dietary energy level. J. Anim. Sci. 37, 1326-1331.
  3. Daniel, Z., Salter, A., and Buttery, P. (2004) Vitamin A regulation of stearoyl-CoA desaturase mRNA levels and fatty acid composition in sheep tissues. Anim. Sci. 78, 237-244.
  4. Gilmour, A., Gogel, B., Cullis, B., and Thompson, R. (2006) ASReml user guide release 2.0. VSN International Ltd., Hemel Hempstead, UK.
  5. Kim, C., Kim, J., Chung, T., and Park, K. (2004) Effects of flaxseed diets on fattening response of Hanwoo cattle: 2. Fatty acid composition of serum and adipose tissues. Asian-Aust. J. Anim. Sci. 17, 1246-1254. https://doi.org/10.5713/ajas.2004.1246
  6. Lee, S., Yoon, D., Choi, N., Hwang, S., Cheong, E., Oh, S., Cheong, I., and Lee, C. (2005) Developmental relationship of unsaturated fatty acid composition and stearoyl-CoA desaturase mRNA level in Hanwoo steers' muscle. Asian-Aust. J. Anim. Sci. 18, 562-566. https://doi.org/10.5713/ajas.2005.562
  7. Mandell, I., Buchanan-Smith, J., and Campbell, C. (1998) Effects of forage vs grain feeding on carcass characteristics, fatty acid composition, and beef quality in Limousin-cross steers when time on feed is controlled. J. Anim. Sci. 76, 2619-2630.
  8. Melton, S. (1990) Effects of feeds on flavor of red meat: a review. J. Anim. Sci. 68, 4421-4435.
  9. Melton, S., Amiri, M., Davis, G., and Backus, W. (1982) Flavor and chemical characteristics of ground beef from grass-, forage-grain-and grain-finished steers. J. Anim. Sci. 55, 77-87.
  10. Nickerson, D., Tobe, V., and Taylor, S. (1997) PolyPhred: automating the detection and genotyping of single nucleotide substitutions using fluorescence-based resequencing. Nucleic Acids Res. 25, 2745-2751. https://doi.org/10.1093/nar/25.14.2745
  11. Perry, D., Nicholls, P., and Thompson, J. (1998) The effect of sire breed on the melting point and fatty acid composition of subcutaneous fat in steers. J. Anim. Sci. 76, 87-95.
  12. Siebert, B., Deland, M., and Pitchford, W. (1996) Breed differences in the fatty acid composition of subcutaneous and intramuscular lipid of early and late maturing, grain-finished cattle. Aust. J. Agric. Res. 47, 943-952. https://doi.org/10.1071/AR9960943
  13. Smith, S., Yang, A., Larsen, T., and Tume, R. (1998) Positional analysis of triacylglycerols from bovine adipose tissue lipids varying in degree of unsaturation. Lipids 33, 197-207. https://doi.org/10.1007/s11745-998-0196-8
  14. Stephens, M., Smith, N., and Donnelly, P. (2001) A new statistical method for haplotype reconstruction from population data. Am. J. Hum. Genet. 68, 978-989. https://doi.org/10.1086/319501
  15. Taniguchi, M., Utsugi, T., Oyama, K., Mannen, H., Kobayashi, M., Tanabe, Y., Ogino, A., and Tsuji, S. (2004) Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle. Mammal. Genome 15, 142-148. https://doi.org/10.1007/s00335-003-2286-8
  16. Woollett, L., Spady, D., and Dietschy, J. (1992) Saturated and unsaturated fatty acids independently regulate low density lipoprotein receptor activity and production rate. J. Lipid Res. 33, 77-88.
  17. Yang, A., Larsen, T., Smith, S., and Tume, R. (1999) $\Delta^{9}$-desaturase activity in bovine subcutaneous adipose tissue of different fatty acid compositiondesaturase activity in bovine subcutaneous adipose tissue of different fatty acid composition. Lipids 34, 971-978. https://doi.org/10.1007/s11745-999-0447-8
  18. Zembayashi, M., Nishimura, K., Lunt, D., and Smith, S. (1995) Effect of breed type and sex on the fatty acid composition of subcutaneous and intramuscular lipids of finishing steers and heifers. J. Anim. Sci. 73, 3325-3332.
  19. Zhang, S., Knight, T., Reecy, J., and Beitz, D. (2008) DNA polymorphisms in bovine fatty acid synthase are associated with beef fatty acid composition. Anim. Genet. 39, 62-70. https://doi.org/10.1111/j.1365-2052.2007.01681.x

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