The Journal of Korean Physical Therapy

The Korean Society of Physical Therapy (대한물리치료학회)
권호 표지

Electrical Stimulation Induces the Collagen Deposition and TGF-${\beta}$1 mRNA Expression in Skin Wound of Rat

  • Lee, Jae-Hyoung (Department of Physical Therapy, Wonkwang Health Science University) ;
  • Park, Chan-Eui (Department of Physical Therapy, College of Physical Therapy, Southwestern University) ;
  • Park, Rae-Joon (Department of Physical Therapy, College of Rehabilitation Sciences, Daegu University)
  • Received : 2010.02.05
  • Accepted : 2010.03.31
  • Published : 2010.06.25
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study was to investigate the effect of electrical stimulation (ES) on the wound closure rate, collagen deposition, and TGF-${\beta}$1 mRNA expression in skin wound of rat. Methods: Twenty male Sprague-Dawley rats (222~271 g) were randomly divided into ES (n=10) and control group (n=10). The ES group received a cathodal stimulation with 50 V at 100 pps for 30 minutes for 7 days, while the control group was not given electrical stimulation. The wound closure rate, collagen density and TGF-${\beta}$1 mRNA ratio were measured. Results: The mean wound closure rates in the ES and control groups were $83.79{\pm}16.35$% and $51.57{\pm}17.76$%, respectively (p<0.001). The collagen density in the ES and control groups were $46.67{\pm}10.68$% and $25.03{\pm}13.09$%, respectively (p<0.001). The TGF-${\beta}$1 mRNA ratio in the ES and control groups were $1.35{\pm}0.60$ and $0.63{\pm}0.30$, respectively at 6 hours post-wound (p<0.01) and $1.69{\pm}0.47$ and $1.32{\pm}0.28$, respectively, at 7 days post-wound (p<0.05). Conclusions: ES accelerated the wound closure rate of skin incision wounds and was accompanied by an increase in collagen deposition in the regenerating dermis. In addition, ES increased TGF-${\beta}$1 mRNA expression during wound healing process. These findings suggest that ES may activate TGF-${\beta}$1 expression, and may increase synthesis activities of fibroblasts in regenerating skin wounds in rats.

Keywords

References

  1. Brown M, McDonnell MK, Menton DN. Electrical stimulation effects on cutaneous wound healing in rabbits. A follow-up study. Phys Ther. 1988;68(6):955-60. https://doi.org/10.1093/ptj/68.6.955
  2. Kloth LC, Feedar JA. Acceleration of wound healing with high voltage, monophasic, pulsed current. Phys Ther. 1988;68(4):503-8. https://doi.org/10.1093/ptj/68.4.503
  3. Fitzgerald GK, Newsome D. Treatment of a large infected thoracic spine wound using high voltage pulsed monophasic current. Phys Ther. 1993;73(6):355-60.. https://doi.org/10.1093/ptj/73.6.355
  4. Burdge JJ, Hartman JF, Wright ML. A retrospective study of high-voltage, pulsed current as an adjunctive therapy in limb salvage for chronic diabetic wounds of the lower extremity. Ostomy Wound Manage. 2009;55(8):30-8.
  5. Harding KG, Morris HL, Patel GK. Science, medicine and the future: healing chronic wounds. BMJ. 2002;324(7330): 160-3. https://doi.org/10.1136/bmj.324.7330.160
  6. Klass BR, Grobbelaar AO, Rolfe KJ. Transforming growth factor beta1 signalling, wound healing and repair: a multifunctional cytokine with clinical implications for wound repair, a delicate balance. Postgrad Med J. 2009;85(999):9-14. https://doi.org/10.1136/pgmj.2008.069831
  7. Riedel K, Riedel F, Goessler UR et al. Tgf-beta antisense therapy increases angiogenic potential in human keratinocytes in vitro. Arch Med Res. 2007;38(1):45-51. https://doi.org/10.1016/j.arcmed.2006.04.010
  8. Barrientos S, Stojadinovic O, Golinko MS et al. Growth factors and cytokines in wound healing. Wound Repair Regen. 2008;16(5):585-601. https://doi.org/10.1111/j.1524-475X.2008.00410.x
  9. Schultz GS, Wysocki A. Interactions between extracellular matrix and growth factors in wound healing. Wound Repair Regen. 2009;17(2):153-62. https://doi.org/10.1111/j.1524-475X.2009.00466.x
  10. Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev 2003;83(3):835-70. https://doi.org/10.1152/physrev.2003.83.3.835
  11. Mani H, Sidhu GS, Kumari R et al. Curcumin differentially regulates TGF-beta1, its receptors and nitric oxide synthase during impaired wound healing. Biofactors. 2002;16(1-2): 29-43. https://doi.org/10.1002/biof.5520160104
  12. Rolfe KJ, Richardson J, Vigor C et al. A role for TGFbeta1- induced cellular responses during wound healing of the non-scarring early human fetus? J Invest Dermatol. 2007; 127(11):2656-67. https://doi.org/10.1038/sj.jid.5700951
  13. Martinez-Ferrer M, Afshar-Sherif AR, Uwamariya C et al. Dermal transforming growth factor-beta responsiveness mediates wound contraction and epithelial closure. Am J Pathol. 2010;176(1):98-107. https://doi.org/10.2353/ajpath.2010.090283
  14. Heldin CH, Eriksson U, Ostman A. New members of the platelet-derived growth factor family of mitogens. Arch Biochem Biophys. 2002;398(2):284-90. https://doi.org/10.1006/abbi.2001.2707
  15. Bourguignon GJ, Bourguignon LY. Electric stimulation of protein and DNA synthesis in human fibroblasts. FASEB J. 1987;1(5):398-402. https://doi.org/10.1096/fasebj.1.5.3678699
  16. Lee JH, Jekal SJ, Park RJ. Enhance of migration and proliferation of cells from tendon biopsies by high voltage pulsed current stimulation. J Kor Soci Phys Ther. 2002;14(2):53-8.
  17. Lee JH, Song IY, Kim JG. Acceleration of wound healing and collagen deposition in rat skin by high voltage pulsed current stimulation. J Kor Soci Phys Ther. 2003;15(4):1-12. https://doi.org/10.1589/jpts.15.1
  18. Jennings J, Chen D, Feldman D. Transcriptional response of dermal fibroblasts in direct current electric fields. Bioelectromagnetics. 2008;29(5):394-405. https://doi.org/10.1002/bem.20408
  19. Bourguignon GJ, Jy W, Bourguignon LY. Electric stimulation of human fibroblasts causes an increase in $Ca^{2+}$ influx and the exposure of additional insulin receptors. J Cell Physiol. 1989;140(2):379-85. https://doi.org/10.1002/jcp.1041400224
  20. Kim YV, Conover DL, Lotz WG et al. Electric field-induced changes in agonist-stimulated calcium fluxes of human HL- 60 leukemia cells. Bioelectromagnetics. 1998;19(6):366-76. https://doi.org/10.1002/(SICI)1521-186X(1998)19:6<366::AID-BEM4>3.0.CO;2-#
  21. Aaron RK, Boyan BD, Ciombor DM et al. Stimulation of growth factor synthesis by electric and electromagnetic fields. Clin Orthop Relat Res. 2004;(419):30-7.
  22. Zhao M. Electrical fields in wound healing-An overriding signal that directs cell migration. Semin Cell Dev Biol. 2009; 20(6):674-82. https://doi.org/10.1016/j.semcdb.2008.12.009