The Journal of Korean Physical Therapy

The Korean Society of Physical Therapy (대한물리치료학회)
권호 표지

Temporal Expression of Interleukin-1${\beta}$ in the Dorsal Root Ganglion in a Rat Model of Lumbar Disc Herniation

  • Kim, Su-Jeong (Institute of Medical Science, Yeungnam University) ;
  • Gu, Hee-Jin (Clinical Trial Center for Medical Devices of Yeungnam University Hospital) ;
  • Cho, Yun-Woo (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Park, Hea-Woon (Department of Rehabilitation Medicine, School of Medicine, Catholic University of Deagu) ;
  • Lee, Joon-Ha (Department of Biochemistry and Molecular Biology, Yeungnam University) ;
  • Hwang, Se-Jin (Department of Anatomy and Cell Biology, College of Medicine, Hanyang University) ;
  • Ahn, Sang-Ho (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University)
  • Received : 2010.05.13
  • Accepted : 2010.06.14
  • Published : 2010.06.25
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: To investigate temporal changes in IL-1${\beta}$ mRNA expression in spinal dorsal horn (DH) and dorsal root ganglion (DRG) in a rat lumbar disc herniation (LDH) model. Methods: Autologous nucleus pulposus, harvested from the tail disc between the second and third coccygeal vertebrae (Co2-3), was implanted next to the left L5 nerve root just proximal to the DRG after partial laminectomy. IL-1${\beta}$ mRNA expression was investigated in DRG and DH in our LDH model. Real-time PCR assays were done using a 7500 Real Time PCR system (Applied Biosystems, USA). Results: Expression of IL-1${\beta}$ in DRG and DH was observed for 30 days postoperatively. Expression of IL-1${\beta}$ mRNA in the ipsilateral DRG of the LDH group gradually increased from 5 to 30 days after surgery. The amount of IL-1${\beta}$ in the contralateral DRG peaked 10 days after surgery and then gradually decreased. However, there was no difference in IL-1${\beta}$ mRNA expression in spinal DH between the LDH group and the sham-operated group. Conclusion: Long-term expression of IL-1${\beta}$ in the LDH model may worsen the chronic pain state. Future studies on inhibition of IL-1${\beta}$ expression in the LDH model will be needed to develop selective treatment strategies for patients with LDH.

Keywords

References

  1. Takahashi H, Suguro T, Okazima Y et al. Inflammatory cytokines in the herniated disc of the lumbar spine. Spine (Phila Pa 1976). 1996;21(2):218-24. https://doi.org/10.1097/00007632-199601150-00011
  2. Le Maitre CL, Freemont AJ, Hoyland JA. The role of interleukin- 1 in the pathogenesis of human intervertebral disc degeneration. Arthritis Res Ther. 2005;7(4):R732-45. https://doi.org/10.1186/ar1732
  3. Ahn SH, Cho YW, Ahn MW et al. mRNA expression of cytokines and chemokines in herniated lumbar intervertebral discs. Spine (Phila Pa 1976). 2002;27(9):911-7. https://doi.org/10.1097/00007632-200205010-00005
  4. Kawakami M, Matsumoto T, Kuribayashi K et al. mRNA expression of interleukins, phospholipase A2, and nitric oxide synthase in the nerve root and dorsal root ganglion induced by autologous nucleus pulposus in the rat. J Orthop Res. 1999;17(6):941-6. https://doi.org/10.1002/jor.1100170620
  5. Ozaktay AC, Kallakuri S, Takebayashi T et al. Effects of interleukin-1 beta, interleukin-6, and tumor necrosis factor on sensitivity of dorsal root ganglion and peripheral receptive fields in rats. Eur Spine J. 2006;15(10):1529-37. https://doi.org/10.1007/s00586-005-0058-8
  6. Ozaktay AC, Cavanaugh JM, Asik I et al. Dorsal root sensitivity to interleukin-1 beta, interleukin-6 and tumor necrosis factor in rats. Eur Spine J. 2002;11(5):467-75. https://doi.org/10.1007/s00586-002-0430-x
  7. Aoki Y, Rydevik B, Kikuchi S et al. Local application of disc-related cytokines on spinal nerve roots. Spine (Phila Pa 1976). 2002;27(15):1614-7. https://doi.org/10.1097/00007632-200208010-00004
  8. Kawakami M, Tamaki T, Hashizume H et al. The role of phospholipase A2 and nitric oxide in pain-related behavior produced by an allograft of intervertebral disc material to the sciatic nerve of the rat. Spine (Phila Pa 1976). 1997;22(10): 1074-9. https://doi.org/10.1097/00007632-199705150-00004
  9. Woolf CJ, Allchorne A, Safieh-Garabedian B et al. Cytokines, nerve growth factor and inflammatory hyperalgesia: the contribution of tumour necrosis factor alpha. Br J Pharmacol. 1997;121(3):417-24. https://doi.org/10.1038/sj.bjp.0701148
  10. Igarashi A, Kikuchi S, Konno S. Correlation between inflammatory cytokines released from the lumbar facet joint tissue and symptoms in degenerative lumbar spinal disorders. J Orthop Sci. 2007;12(2):154-60. https://doi.org/10.1007/s00776-006-1105-y
  11. Jeanjean AP, Moussaoui SM, Maloteaux JM et al. Interleukin- 1 beta induces long-term increase of axonally transported opiate receptors and substance P. Neuroscience. 1995; 68(1):151-7. https://doi.org/10.1016/0306-4522(95)00106-S
  12. Molenaar GJ, Berkenbosch F, van Dam AM et al. Distribution of interleukin 1 beta immunoreactivity within the porcine hypothalamus. Brain Res. 1993;608(1):169-74. https://doi.org/10.1016/0006-8993(93)90791-K
  13. Watt JA, Hobbs NK. Interleukin-1beta immunoreactivity in identified neurons of the rat magnocellular neurosecretory system: evidence for activity-dependent release. J Neurosci Res. 2000;60(4):478-89. https://doi.org/10.1002/(SICI)1097-4547(20000515)60:4<478::AID-JNR6>3.0.CO;2-R
  14. Copray JC, Mantingh I, Brouwer N et al. Expression of interleukin-1 beta in rat dorsal root ganglia. J Neuroimmunol. 2001;118(2):203-11. https://doi.org/10.1016/S0165-5728(01)00324-1
  15. Eliav E, Benoliel R, Herzberg U et al. The role of IL-6 and IL-1beta in painful perineural inflammatory neuritis. Brain Behav Immun. 2009;23(4):474-84. https://doi.org/10.1016/j.bbi.2009.01.012
  16. Cho YW, Park HW, Kim SJ et al. Expression of MCP‐ in spinal dorsal horn in a rat model of lumbar disc herniation. J Kor Soc Phys Ther. 2009;21(3):95-102.
  17. Doita M, Kanatani T, Harada T et al. Immunohistologic study of the ruptured intervertebral disc of the lumbar spine. Spine (Phila Pa 1976). 1996;21(2):235-41. https://doi.org/10.1097/00007632-199601150-00015
  18. Feghali CA, Wright TM. Cytokines in acute and chronic inflammation. Front Biosci. 1997;2:d12-26. https://doi.org/10.2741/A171
  19. Ferreira SH, Lorenzetti BB, Bristow AF et al. Interleukin-1 beta as a potent hyperalgesic agent antagonized by a tripeptide analogue. Nature. 1988;334(6184):698-700. https://doi.org/10.1038/334698a0
  20. Reeve AJ, Patel S, Fox A et al. Intrathecally administered endotoxin or cytokines produce allodynia, hyperalgesia and changes in spinal cord neuronal responses to nociceptivestimuli in the rat. Eur J Pain. 2000;4(3):247-57. https://doi.org/10.1053/eujp.2000.0177
  21. Kim WR, Ahn SH, Cho YW et al. The long-lasting pattern of the neuropathic radicular pain in an autologous nucleus pulposus model of rat. J Korean Acad Rehabil Med. 2009; 33(4):477-82.
  22. Li WW, Sabsovich I, Guo TZ et al. The role of enhanced cutaneous IL-1beta signaling in a rat tibia fracture model of complex regional pain syndrome. Pain. 2009;144(3):303-13. https://doi.org/10.1016/j.pain.2009.04.033
  23. Terkeltaub R, Sundy JS, Schumacher HR et al. The interleukin 1 inhibitor rilonacept in treatment of chronic gouty arthritis: results of a placebo-controlled, monosequence crossover, non-randomised, single-blind pilot study. Ann Rheum Dis. 2009;68(10):1613-7. https://doi.org/10.1136/ard.2009.108936
  24. Koch H, Reinecke JA, Meijer H et al. Spontaneous secretion of interleukin 1 receptorantagonist (IL-1ra) by cells isolated from herniated lumbar discal tissue after discectomy. Cytokine. 1998;10(9):703-5. https://doi.org/10.1006/cyto.1998.0353
  25. Liu T, van Rooijen N, Tracey DJ. Depletion of macrophages reduces axonal degeneration and hyperalgesia following nerve injury. Pain. 2000;86(1-2):25-32. https://doi.org/10.1016/S0304-3959(99)00306-1
  26. Myers RR, Campana WM, Shubayev VI. The role of neuroinflammation in neuropathic pain: mechanisms and therapeutic targets. Drug Discov Today. 2006;11(1-2):8-20. https://doi.org/10.1016/S1359-6446(05)03637-8
  27. Kiguchi N, Maeda T, Kobayashi Y et al. Macrophage inflammatory protein-1alpha mediates the development of neuropathic pain following peripheral nerve injury through interleukin-1beta up-regulation. Pain. 149(2):305-15. https://doi.org/10.1016/j.pain.2010.02.025
  28. Yoshida M, Nakamura T, Sei A et al. Intervertebral disc cells produce tumor necrosis factor alpha, interleukin-1beta, and monocyte chemoattractant protein-1 immediately after herniation: an experimental study using a new hernia model. Spine (Phila Pa 1976). 2005;30(1):55-61. https://doi.org/10.1097/01.brs.0000149194.17891.bf
  29. Watkins LR, Maier SF, Goehler LE. Cytokine-to-brain communication: a review & analysis of alternative mechanisms. Life Sci. 1995;57(11):1011-26. https://doi.org/10.1016/0024-3205(95)02047-M
  30. Constandil L, Hernandez A, Pelissier T et al. Effect of interleukin-1beta on spinal cord nociceptive transmission of normal and monoarthritic rats after disruption of glial function. Arthritis Res Ther. 2009;11(4):R105. https://doi.org/10.1186/ar2756
  31. Watkins LR, Maier SF. Beyond neurons: evidence that immune and glial cells contribute to pathological pain states. Physiol Rev. 2002;82(4):981-1011. https://doi.org/10.1152/physrev.00011.2002
  32. Takebayashi T, Cavanaugh JM, Cuneyt Ozaktay A et al. Effect of nucleus pulposus on the neural activity of dorsal root ganglion. Spine (Phila Pa 1976). 2001;26(8):940-5. https://doi.org/10.1097/00007632-200104150-00018
  33. Pineau I, Lacroix S. Proinflammatory cytokine synthesis in the injured mouse spinal cord: multiphasic expression pattern and identification of the cell types involved. J Comp Neurol. 2007;500(2):267-85. https://doi.org/10.1002/cne.21149
  34. Martucci C, Trovato AE, Costa B et al. The purinergic antagonist PPADS reduces pain related behaviours and interleukin-1 beta, interleukin-6, iNOS and nNOS overproduction in central and peripheral nervous system after peripheral neuropathy in mice. Pain. 2008;137(1):81-95. https://doi.org/10.1016/j.pain.2007.08.017
  35. Ledeboer A, Sloane EM, Milligan ED et al. Minocycline attenuates mechanical allodynia and proinflammatory cytokine expression in rat models of pain facilitation. Pain. 2005; 115(1-2):71-83. https://doi.org/10.1016/j.pain.2005.02.009
  36. Fiorentino PM, Tallents RH, Miller JN et al. Spinal interleukin- 1beta in a mouse model of arthritis and joint pain. Arthritis Rheum. 2008;58(10):3100-9. https://doi.org/10.1002/art.23866