Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antibacterial and Antioxidant Activities of Solvent Extracts from Different Parts of Hagocho (Prunella vulgaris)

하고초 부위별 용매추출물의 항균 및 항산화 활성

  • Seo, Jong-Kwon (Dept. of Food Science, International University of Korea) ;
  • Kang, Min-Jung (Namhae Garlic Research Institute) ;
  • Shin, Jung-Hye (Namhae Garlic Research Institute) ;
  • Lee, Soo-Jung (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Hey-Gwang (Dept. of Toxicology, College of Pharmacy, Chungnam National University) ;
  • Sung, Nak-Ju (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Chung, Young-Chul (Dept. of Food Science, International University of Korea)
  • 서종권 (한국국제대학교 식품과학부) ;
  • 강민정 ((재)남해마늘연구소) ;
  • 신정혜 ((재)남해마늘연구소) ;
  • 이수정 (경상대학교 식품영양학과.농업생명과학연구원) ;
  • 정혜광 (충남대학교 약학대학) ;
  • 성낙주 (경상대학교 식품영양학과.농업생명과학연구원) ;
  • 정영철 (한국국제대학교 식품과학부)
  • Received : 2010.07.07
  • Accepted : 2010.09.28
  • Published : 2010.10.31
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Abstract

This study was designed to investigate the effects of antioxidant and antibacterial activities of water, ethanol and methanol extracts from root, stem and flower stalk of Hagocho (Prunella vulgaris). The solvent extract yields from root were higher than those from stem and flower stalk, and water extract showed the highest extraction yield against ethanol and methanol extracts. The contents of total phenolic and flavonoid in ethanol extract were significantly higher in stem extract compared with those of root and flower stalk. In the case of water and methanol extracts, however, the contents were the highest in flower stalk. The electron donating ability and reducing power in all test groups were significantly increased in a dose-dependent manner, and antioxidant activities were the highest in methanol extract. In extracts from different parts of Hagocho, the antioxidant activity was the highest in flower stalk followed by stem and root. ABTS radical scavenging ability of water and methanol extracts was above 50% at $100\;{\mu}g/mL$ concentration. Antibacterial activities did not show significant differences depending on parts of Hagocho. However, antibacterial activity of ethanol extract was higher than those of other extracts.

식물체의 부위별 및 추출용매에 따른 생리활성을 비교하고자 하고초를 뿌리, 줄기 및 꽃대로 구분하고 에탄올, 메탄올 및 물 추출물에 대한 항산화 및 항균 활성을 비교 분석하였다. 추출 수율은 뿌리 추출물이 다소 높은 경향을 보였으며, 물 추출물은 에탄올 및 메탄올 추출물에 비해 6~11배의 높은 수율을 나타내었다. 총 페놀화합물 함량은 에탄올 추출물에서는 줄기에서, 메탄올과 물 추출물에서는 꽃대에서 유의적으로 높게 나타났으며, 플라보노이드 함량은 뿌리에 비해 줄기와 꽃대에서 전반적으로 높은 함량을 나타내었다. 각 추출물의 전자공여능 및 환원력은 모든 실험군에서 농도 의존적으로 증가하였고, 용매별 추출물에서는 메탄올 추출물의 활성이 유의적으로 높게 나타났으며, 부위별 추출물에서는 꽃대> 줄기> 뿌리의 순으로 높은 전자공여능 및 환원력을 보였다. 또한 메탄올 추출물과 물 추출물의 ABTS 라디칼 소거능은 $100\;{\mu}g/mL$의 저농도에서도 꽃대, 줄기 및 뿌리에서 모두 50% 이상의 소거능을 보였다. 하고초 각 부위별 추출물의 항균활성을 측정한 결과 꽃대, 줄기 및 뿌리간의 유의적인 차이는 보이지 않았으나, 열수 추출물에 비해 에탄올 및 메탄올 추출물에서 다소 높은 항균활성이 관찰되었는데, 특히 뿌리와 줄기의 $1,000\;{\mu}g/mL$ 농도에서는 유의적으로 높은 항균활성이 확인되었다. 이상의 결과로 볼 때, 하고초의 꽃대를 이용한 에탄올 및 메탄올 추출물은 식품의 저장성 향상에 이용가치가 높을 것으로 기대된다.

Keywords

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