Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Phyto-Extract Mixture on Adiposity and Serum Lipid Levels in Obese Mice Induced by High Fat Diet

고지방식이로 유도된 비만쥐에서 식물혼합 추출물의 체지방 형성 및 혈청 지질에 미치는 영향

  • 장영선 ((주)벤스랩 중앙연구소) ;
  • 정종문 (수원대학교 생명과학과)
  • Received : 2010.07.05
  • Accepted : 2010.09.09
  • Published : 2010.10.31
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Abstract

In this study, we investigated the inhibition of phyto-extract mixture (PEM) in several digestive enzymes ($\alpha$-amylase, $\alpha$-glucosidase and lipase) for anti-obesity. The current study also examined the effects of PEM on adiposity and serum lipid levels in obese mice fed with high fat diet. ICR male mice weighing $33{\pm}1.1\;g$ were randomly divided into three groups, one normal diet group (control, ND group) and two high fat diet groups with or without PEM supplement (HFD group and PEM group). The mice were fed the PEM experimental for 6 weeks and then they were sacrificed. The results showed that the final weight, weight gain, food efficiency ratio and body fat were decreased by the addition of PEM compared to those of HFD group. White adipose tissue weights of epididymal, mesenteric and retroperitoneal areas in the PEM group were reduced to 31.2%, 8.8%, and 37.8%, respectively, compared to the HFD group. The levels of serum triglyceride, total cholesterol, LDL-cholesterol in the PEM group were significantly lower than those of HFD group. The body weight gain and food efficiency ratio of PEM group were significantly lower compared with those of HFD group. From the above results, the PEM may be effective material for anti-obesity through reducing serum triglyceride and body fats as well as decreasing body weight.

본 연구에서는 체중조절 효과가 우수하고, 부작용이 없는 천연소재를 개발하기 위해 문헌 조사 및 소화 효소 억제실험을 통하여 녹차잎 추출물, 포도씨 추출물, 상엽추출물을 소재로 하여 체중조절 효과가 우수하고 체지방 개선 효능이 있는 식물혼합 추출물(PEM)을 선별하였다. 고지방식이에 의해 유도된 비만 마우스의 몸무게, 지방조직 무게(부고환, 복막후, 장간막 지방) 및 혈청 지질 농도에 식물혼합 추출물이 어떤 영향들을 미치는지 연구하기 위하여, 6주령의 ICR 마우스를 1주일간 적응시킨 후 정상식이를 급여한 정상식이군, 고지방식이(lard 20%; 200 g/kg)를 급여한 고지방식이군과 고지방식이에 식물혼합 추출물을 1%(10 g/kg)을 급여한 PEM군으로 나누어 6주간 사육하였다. 고지방식이를 섭취한 군은 정상식이를 섭취한 정상식이군과 비교하여 혈청 지질 수준, 몸무게, 지방조직 무게가 현저하게 증가하였다. 반면 식물혼합 추출물을 함유하고 있는 식이는 몸무게, 지방조직 무게 및 혈중 총 콜레스테롤, LDL-콜레스테롤, 그리고 중성지방을 고지방식이와 비교하여 현저하게 감소시키는 것을 알 수 있다. 결론적으로 고지방식이 급여로 증가된 체중 및 지방조직 무게를 감소시키고, 혈청지질 조성을 개선시킨 식물혼합 추출물은 고지방 식사로 인한 고지혈증 및 비만과 같은 지질대사 이상을 개선시키는데 긍정적인 영향을 나타내므로 항비만용 건강기능성 식품 소재로써 이용될 가능성을 시사하고 있다.

Keywords

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