Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Healing Effects of Ginsenoside Rg1 on Experimental Open Wound in Rat

흰쥐의 외과적 창상에 대한 Ginsenoside Rg1의 치료효과

  • 임애경 ((재)대구테크노파크 바이오산업지원센터) ;
  • 김길수 (경북대학교 수의과대학) ;
  • 박수정 ((재)대구테크노파크 바이오산업지원센터) ;
  • 홍주헌 (대구가톨릭대학교 외식식품산업학부) ;
  • 최향자 (소리소 화장품) ;
  • 김대익 ((재)대구테크노파크 바이오산업지원센터)
  • Received : 2010.03.19
  • Accepted : 2010.10.11
  • Published : 2010.10.31
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Abstract

This study was performed to investigate the effect of ginsenoside Rg1 treatment on wound healing using SD rats by generating four full-thickness skin wounds on the dorsum. In the Rg1-treated groups (5,000 and 10,000 ppm), area of wounds and macroscopic inflammatory signs were significantly decreased compared to control group throughout the experimental period in a concentration dependent manner. Histological appearance after 20 days of treatment with Rg1 revealed the formation of epithelial layer, hair follicles and progressive angiogenesis and an increase in collagen and granulation as compared to control group. Rg1 treatment resulted in the increased expression of the vascular endothelial growth factor (VEGF) mRNA and reduced expression of transforming growth factor beta (TGF-$\beta$) mRNA in wounded skin compared to control group. The expression levels of VEGF and TGF-$\beta$ mRNA in the Rg1-treated groups were similar to those of Fucidin(R) ointment-treated group. These results suggested that Rg1 should be helpful for the promotion of wound healing.

상처치료 과정 중 ginsenoside Rg1(5,000 ppm, 10,000 ppm) 처리에 의한 효력을 평가하기 위해 SD rats의 등쪽 피부에 biopsy punch를 이용한 4개의 창상을 유발 후, 이 과정에서 일어나는 피부조직의 변화를 관찰하였다. 상처에 Rg1의 도포로 인해 창상치료과정이 활발해짐을 관찰할 수 있었다. Rg1을 처리한 군에서 절개된 상처의 표피화와 수축이 더 빨라졌고 창상면적에 있어서도 양성대조군인 시판연고 도포군과 비교하여 유의하게 줄어들었다. 염색한 조직의 현미경 관찰 결과에서는 정상대조군에서 볼 수 있는 완전한 상피층은 찾을 수 없었으나 Rg1 처리군에서 모낭과 신생혈관이 관찰되었고 상피층의 회복조짐도 관찰되었다. 병변의 TGF-$\beta$ 및 VEGF의 발현량을 조사한 결과 Rg1의 도포에 의하여 더 많은 VEGF의 발현을 유도하고 TGF-$\beta$의 활성화를 차단하여 반흔 형성을 감소시켰다. 이러한 결과를 종합하여 Rg1은 흰쥐의 외과적 창상에 치료효과가 있다고 사료된다.

Keywords

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