Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Prevalence and molecular characterization of tetracycline-resistant Enterococcus isolates from livestock

가축에서 테트라사이클린 내성 장구균 조사 및 분자생물학적 특성규명

  • 김철민 (전라북도축산위생연구소) ;
  • 강수진 (전라북도축산위생연구소) ;
  • 이병종 (전라북도축산위생연구소) ;
  • 이성재 (전라북도축산위생연구소) ;
  • 육대수 (전라북도축산위생연구소)
  • Received : 2010.05.19
  • Accepted : 2010.06.08
  • Published : 2010.06.30
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Abstract

BIn the present study, Enterococcus isolates originating from livestock were studied for the phenotypic and genotypic assessment of tetracycline resistance. A total of 74 isolates encompassing the species Enterococcus faecalis (n=12) and E. faecium (n=62) displayed phenotypic resistance to tetracycline. Tetracycline resistance gene [tet (M), 1,886bp] were sequenced by dye terminator cycle sequencing method and compared with tet (M) sequences available from the GenBank database. Sequencing analysis of PCR amplicons showed high homology to the reference strains ranging 97.2~100%. The tet (M) genes were divided into three major subgroups according to phylogenetic analysis. The genetic information obtained from this study could be useful for the molecular study of enterococci.

Keywords

References

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