Changes in Water Potential of Pine Seedlings Inoculated with Bursaphelenchus xylophilus

소나무재선충 접종목의 수분포텐셜 변화

  • Received : 2010.03.05
  • Accepted : 2010.05.27
  • Published : 2010.06.30

Abstract

The purpose of this study was to understand water stress development in pine seedlings inoculated with Bursaphelenchus xylophilus. Leaf water potentials of four years old Pinus densiflora seedlings inoculated with the nematode were measured with a plant moisture system at predawn and midday. The midday leaf water potentials on the 35th day after the inoculation were -1.04 MPa in the infected seedlings, while -0.94 MPa in the non-infected seedlings. The predawn water potentials on the 56th day were -0.71 MPa in the infected, while -0.26 MPa in the non-infected. At this time the leaves of the infected seedlings became yellow and resin did not exude on the cut basal stem. Density of the nematode was maximum on the 28th day, but dropped sharply on the 68th day. The phloem and pith tissues became brown and the resin exudation reduced in the infected seedlings. In conclusion, pinewood nematode inoculation developed water stress in pine seedlings in a month.

소나무재선충을 접종한 소나무묘목에서 잎의 수분포텐셜 변화를 식물수분시스템으로 측정하였다. 소나무재선충 접종 후 35일째 12시경에 소나무의 잎 수분포텐셜은 소나무소나무재선충 접종묘에서 -1.04 MPa, 비접종묘에서 -0.94 MPa로 소나무재선충 접종묘가 비접종묘보다 더 낮았다. 접종 후 56일째에는 해뜨기 직전 잎 수분포텐셜은 소나무재선충 접종묘가 -0.71 MPa, 비접종묘가 -0.26 MPa로 소나무재선충 접종묘가 더 낮았다. 접종 후 63일째에는 소나무소나무재선충 접종묘의 1년생 잎이 옅은 황색으로 되었다. 이때 접종묘의 근원부 단면에서는 수지가 나오지 않았다. 소나무재선충의 밀도는 접종 후 28일째에 최대가 되었다가 68일째에 급감하였다. 접종묘의 체관부와 수조직은 갈변되었으며 수지 유출은 접종 후 계속 감소하였다. 결론적으로 소나무재선충은 접종 1개월부터 소나무묘목에 수분 스트레스를 일으켰다.

Keywords

References

  1. 윤준혁, 우관수, 문일성, 구영본, 이도형. 2008. 소나무재선충 인공접종에 의한 해송묘목의 병 발달 및 수분함량변화. 한국임학회지 97: 570-575.
  2. 이경준. 2007. 수목생리학. 서울대학교출판부. 서울. pp. 514.
  3. 이상명, 이상길, 김철수, 문일성, 김준범, 박남창, 김동수, 전향미, 이상태, 노희선, 허혜순. 2006. 소나무재선충병 바로 알기. 국립산림과학원. 서울. pp. 93.
  4. 이상명, 추호렬, 박남창, 문일성, 김준범. 1990. 고사목에서 분리된 선충과 곤충의 종류 및 솔수염하늘소 부위별 소나무재선충 밀도조사. 한국응용곤충학회지 29: 14-19.
  5. 이상명, 하현보. 2004. 알기쉬운 소나무재선충. 국립산림과학원. 서울. pp. 104.
  6. 임업연구원. 1990. 일본의 소나무재선충병 연구. 임업연구원연구자료 제48호, pp. 239.
  7. 福田 健二, 鈴木 和夫. 1988. マツ材線病の病の進展と水分生理特性の化. 日本林學會誌 70: 390-394.
  8. 須川 豊伸. 1982. マツノザイセンチュウ被害による傷害樹脂道の生と柔細胞の性. 日本林學會誌 64: 112-116.
  9. 原 直樹. 二井 一禎. 2001. マツノザイセンチュウ感染に よるクロマツ木部放射柔細胞の化と周道管への影響. 日本林學會誌 83: 285-289.
  10. 黑田 慶子. 伊藤 進一郞. 1992. クロマツに侵入後のマツノザイセンチュウの動きとその他の微生物相の遷. 日本林學會誌 74: 383-389.
  11. Ameglio, T. Archer, P. Cohen, M. Valancogne, C. Daudet, F. Dayau and S. Cruiziat, P. 1999. Significance and limits in the use of predawn leaf water potential for tree irrigation. Plant and Soil 207: 155-167.
  12. Dwinell, L.D. 1997. The pinewood nematode: regulation and mitigation. Annual Review of Phytopathology 35: 153-46. https://doi.org/10.1146/annurev.phyto.35.1.153
  13. Fukuda, K. 1997. Physiological process of the symptom development and resistance mechanism in pine wilt disease. Journal of Forest Research 2: 171-181. https://doi.org/10.1007/BF02348216
  14. Fukuda, K. Hogetsu, T. and Suzuki, K. 1992. Photosynthesis and water status of pine-wood nematode-infected pine seedlings. Journal of the Japanese Forest Society 74: 1-8.
  15. Hsiao, T. and Acevedo, E. 1974. Plant responses to water deficits water-use efficiency and drought resistance. Agricultural Meteorolgy 14: 59-84. https://doi.org/10.1016/0002-1571(74)90011-9
  16. Hara, N. Takeuch, Y. and Futai, K. 2006. Cytological changes in ray parenchyma cells of seedlings of three pine species infected with the pine wilt disease. Japanese Journal of Nematology 36: 23-32. https://doi.org/10.3725/jjn.36.23
  17. Ichihara, Y. Fukuda, K. and Suzuki, K. 2000. Early symptom development and histological changes associated with migration of Bursaphelenchus xylophilus in seedling tissues of Pinus thunbergii. Plant Disease 84: 675-680. https://doi.org/10.1094/PDIS.2000.84.6.675
  18. Kozlowski, T.T. and Pallardy, S.G. 1997. Physiology of woody plants. 2nd ed. Academic Press. London, pp.411.
  19. Kuroda, K. Yamada, T. Mineo, K. and Tamura, H. 1988. Effect of cavitation on the development of the pine wilt disease caused by Bursaphelenchus xylophilus. Annals of the Phytopathological Society of Japan 54: 606-615. https://doi.org/10.3186/jjphytopath.54.606
  20. Nobuchi, T. Tominaga, T. Futai, K. and Harada, H. 1984. Cytological study of pathological changes in japanese black pine (Pinus thunbergii) seedlings after inoculation with pinewood nematode (Bursaphelenchus xylophilus). Bull Kyoto Univ For 56: 224-233.
  21. Taize, L. Zeiger, E.(전방욱 번역). 2005, 식물생리학(3판), 라이프사이언스, 서울. pp. 637.
  22. Tan, J.-J. Ye, J.-R. Wu, X.-Q. Zhu, Y.-F. and Li Y. 2005. A Study on disease development and early diagnosis of pine wood nematode, Bursaphelenchus xylophilus, infection of japanese black pine. Nematology 7: 481-485. https://doi.org/10.1163/156854105774384787