The Influence of Germinations in Soaking Treatment of Rhus chinensis, Lespedeza cyrtobotrya and Lespedeza cuneata

붉나무.참싸리.비수리 종자의 침지 처리가 발아에 미치는 영향

  • Hur, Young-Jin (Department of Environment Landscape Architecture, Graduate School, Dankook University) ;
  • Kim, Min-Ho (Department of Microbiology, College of Advanced Sciences, Dankook University) ;
  • Cha, Go-Woon (Department of Microbiology, College of Advanced Sciences, Dankook University) ;
  • Ahn, Tae-Young (Department of Microbiology, College of Advanced Sciences, Dankook University)
  • 허영진 (단국대학교 대학원 환경조경학과) ;
  • 김민호 (단국대학교 미생물학과) ;
  • 차고운 (단국대학교 미생물학과) ;
  • 안태영 (단국대학교 미생물학과)
  • Received : 2009.12.10
  • Accepted : 2010.04.02
  • Published : 2010.04.01

Abstract

Herbs and shrubs are employed for environmental restoration purposes. Among common herbs and shrubs, few species with low germination rates were selected and studied for enhanced germination rates and decreased germination times via soaking treatment. Rhus chinensis, incubator grown samples treated with the bacterial solution for 72hrs followed by immediate seeding showed the highest germination rate of 26.7% and germination period of 5.7 days, 3 days decrease from the control. Treatment of distilled water (t=3.79, p<0.01), nutrient broth (t=4.44, p<0.00) and bacterial solution (t=4.42, p<0.00) showed highly significant difference. In the case of soil tests, treating in the nutrient broth for 72 hrs followed by immediate seeding yielded the the highest germination rate of 23.3% with 7.3 days to initial germination, a decrease of 14.7 days with respect to the control. All the samples followed by immediate seeding showed significant difference (t=2.13, p<0.05). Incubator grown samples of Lespedeza cyrtobotrya showed different results. The incubator samples suspended for 48 hrs in distilled water followed by immediate seeding and 1 day drying displayed the highest germination rate of 96.7%, surpassing that of the control by 33.4%. The incubator samples treated with the distilled water and nutrient broth showed enhanced germination. But only the samples treated with distilled water and nutrient broth for 48hrs showed the increased germination in soil tests. All the sample treated for 24 hrs followed by immediate seeding or dried for 1 day showed initial germination as early as 1 day in incubator. The initial germinations were shortened in the samples treated with distilled water and nutrient broth for 48hrs in soil tests. Lespedeza cuneata incubator sample treated with nutrient broth for 24 hrs and dried for 1 day exhibited the highest germination rate of 83.3%, a 31.1% improvement over the control. The incubator samples treated with distilled water for 48 hrs (t=4.20, p<0.01) showed effective increase of germination. The treatment of distilled water (t=2.96, p<0.05) and bacterial solution (t=2.24, p<0.05) showed significant difference. The germination rates in soil were less than those of incubator and the control. The incubator samples treated with distilled water and bacterial solution displayed 1 day germination period, shortened by 1.3 days compared to the control. For soil grown samples, the samples treated with distilled water showed delayed initial germination and those treated with nutrient broth for 48hrs and bacterial solution for 72hrs shortened initial germination.

Keywords

References

  1. 국토해양부. 2009. 도로비탈면 녹화공사의 설계 및 시공지침.
  2. 김남춘. 1997a. 사면녹화공사용 자생목본의 파종 적기에 관한 연구. 한국조경학회지 25(1) : 73-81.
  3. 김남춘. 1997b. 주요 초본식물의 비탈면 파종적기에 관한 연구. 한국조경학회지 25(2) : 62-72.
  4. 김정훈. 2005. 비탈면녹화를 위한 식생배합개선 방안 연구. 한국환경복원녹화기술학회지 8(2) : 90-97.
  5. 차고운․허영진․안태영. 2008. 미생물의 의한 자귀나무․참싸리․비수리 종자의 발아촉진. 한국환경복원녹화기술학회지 11(3) : 107-115.
  6. 허영진․안태영. 2006. 훼손 비탈면의 생태복원 녹화를 위한 종자배합량의 계절별 가중치에 관한 연구. 한국환경복원녹화기술학회지 9(1) : 41-54.
  7. 홍성각. 2002. 산불 적응 수종의 종자 발아 특성. 학술원논문집(자연대학편) 41(1) : 117-135
  8. 환경부. 2001. 생태적측면의 절개비탈면 녹화공법 활성화 방안에 관한 연구.
  9. 환경부. 2007. 재해 및 개발로 훼손된 국토핵심생태녹지축지역의 지형 및 수림복원 기술개발. 환경부 차세대 핵심환경기술개발 사업 1차년도 보고서. pp.54-64.
  10. Dell, B. 1980. Structure and Function of the Strophiolan Plug in Seeds of Abizzia lophantha. Am. J. Botany, 67(4) : 556-563. https://doi.org/10.2307/2442296
  11. Lee, S. W., H. W. Oh., K. H. Lee and T. Y. Ahn. 2009. Methylobacterium dankookense sp. nov. isolated from drinking water. J. Microbiol, 47(6) : 716-720. https://doi.org/10.1007/s12275-009-0126-6
  12. Somova, L. A., N. S. Pechurkin., A. B. Sarangova and T. I. Pisman. 2001. Effect of bacterial population density on germination wheat seeds and dynamics of simple artificial ecosystems. Ade. Space Res, 27(9) : 1611-1615. https://doi.org/10.1016/S0273-1177(01)00257-5
  13. Koller, D. 1972. Environmental Control of Seed Germination. In Seed Biology. VolII. Ed. by Kozlowski, T.T. Acad. Press. 1-101.
  14. 最新斜面土留め技術總攬委員會. 1991. 最新斜面 土留め技術總攬 資料編.