The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

Antitumor and Immunomodulatory Activities of Platycodon grandiflorum

길경(桔梗)이 면역활성(免疫活性) 및 항암(抗癌)에 미치는 영향

  • Lee, Ji-Yung (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Jin-Moo (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Chang-Hoon (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Cho, Jung-Hoon (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Jang, Jun-Bock (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Kyung-Sub (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University)
  • 이지영 (경희대학교 대학원 한의학과 부인과학교실) ;
  • 이진무 (경희대학교 대학원 한의학과 부인과학교실) ;
  • 이창훈 (경희대학교 대학원 한의학과 부인과학교실) ;
  • 조정훈 (경희대학교 대학원 한의학과 부인과학교실) ;
  • 장준복 (경희대학교 대학원 한의학과 부인과학교실) ;
  • 이경섭 (경희대학교 대학원 한의학과 부인과학교실)
  • Received : 2010.10.21
  • Accepted : 2010.11.09
  • Published : 2010.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Methods: Intravenous administration of Platycodon grandiflorum was performed 2 days before tumor inoculation, then mice were killed 14 days after tumor inoculation, then number of tumor colonies were counted. Methanol extracts of Platycodon grandiflorum was added to colon26-M3.1 carcinoma cells, L5178Y-R lymphoma cells and Hela cells, and then cell growth was counted. To observe the immunomodulating effects of Platycodon grandiflorum, production of IL-6, IL-10, IL-12 and TNF-$\alpha$ were measured with ELISA assay and the cell growth of macrophage were also counted. Furthermore, antimetastatic experiment after depletion NK cells by injection of anti-asialo GM1 serum was also administered. Results: Intravenous administration of Platycodon grandiflorum significantly inhibited metastasis of colon26-M3.1 carcinoma cells. In an in vitro cytotoxicity analysis, Platycodon grandiflorum affected tumor cell growth above specific concentration. As compared with control, the production of IL-6, IL-10, IL-12 and TNF-$\alpha$ were incresed. And depletion NK cell completly abolished the inhibitory effect of metastasis. Conclusion: Platycodon grandiflorum appears to have considerable activity on immunomodulating effects and inhibit the metastasis of tumor. Further evaluation is needed for settling this.

Keywords

References

  1. 서울대학교 의과대학. 종양학. 서울: 서울대학교출판부. 1993:1, 225, 227.
  2. 해리스내과학 편집위원회. Harrison's 내과학 16th edition. 서울:도서출판 MIP. 2006:489.
  3. 한의부인과학 교재편찬위원회. 한의부인과학 상. 서울:정담. 2002:303-7.
  4. 배우진 등. 天南星이 HeLa cell의 증식억제와 apoptosis에 미치는 영향. 대한한방부인과학회지. 2006;19(3):25-40.
  5. 박명원 등. 當歸芍藥散이 HeLa cell의 增殖抑制와 死滅에 미치는 影響. 대한한방부인과학회지. 2006;19(2):34-48.
  6. 강용구 등. 桃核承氣湯이 子宮頸部癌細胞(HeLa cell)의 apoptosis에 미치는 影響. 대한한방부인과학회지. 2006; 19(2):77-91.
  7. 박경미 등. 子宮頸部癌細胞(HeLa cell)에서 乳香의 細胞枯死機轉硏究. 대한한방부인과학회지. 2006;19(2):92-106.
  8. 한의과대학 본초학 편찬위원회. 本草學. 서울:永林社. 2007:496-7.
  9. 박재갑 등. 종양학. 서울:일조각. 2003:3.
  10. Abbas L. Cellular and Molecular Immunology 5th edition. Korea:W.B. Saunders a division of Elsevier Science. 2003:247-263, 391, 400, 402.
  11. Martin DA et al. Clinical oncology. USA:Elsevier Churchill Livingstone. 2004:59.
  12. 김영섭 등. 다년생 도라지의 항암 및 면역활성. 약회회지. 1998;42(4):382-7.
  13. Lee KJ et al. Inhibition of tumor invasion and metastasis by aqueous extract of the radix of Platycodon grandiflorum. Food Chem Toxicol. 2006;44(11):1890-6. https://doi.org/10.1016/j.fct.2006.06.009
  14. Lee KJ et al. Saponins derived from the roots of Platycodon grandiflorum inhibit HT-1080 cell invasion and MMPs activities: Regulation of NF -kappaB activation via ROS signal pathway. Cancer Lett. 2008;268(2) :233-43. https://doi.org/10.1016/j.canlet.2008.03.058
  15. 박재우, 윤성우. 중국의 암치료 현황. 대한암한의학회지. 2006;11(1):65-73.
  16. 조종관. 한방임상종양학. 대전:주민출판사. 2001:9-15.
  17. 吳普. 神農本草經. 서울:醫聖堂. 2003 :246.
  18. 이상인 등. 漢藥臨床應用. 서울:傳統醫學硏究所. 1998:472-3.
  19. 박치영 등. 桔梗약침의 천식억제 및 면역조절효과에 대한 실험적 연구. 대한침구학회지. 2005;22(6):61-74.
  20. 박상현 등. 마행감석탕과 桔梗이 인간기관지 상피세포의 Cytokine에 미치는 영향. 경희의학. 2001;17(2):230-41.
  21. Kim JY et al. Inhibition of tumor necrosis factor-alpha-induced expression of adhesion molecules in human endothelial cells by the saponins derived from roots of Platycodon grandiflorum. Toxicol Appl Pharmacol. 2006;210:150-6. https://doi.org/10.1016/j.taap.2005.09.015
  22. 이지영등. 도라지(Platycodon grandiflorum DC)추출 성분의 암세포 증식 억제 효과. 한국식품과학회지. 1998;30(1) :13-21.
  23. Kim MO et al. Platycodin D induces mitotic arrest in vitro, leading to endoreduplication, inhibition of proliferation and apoptosis in leukemia cells. Int J Cancer. 2008;122(12) :2674-81. https://doi.org/10.1002/ijc.23442
  24. Willian EP. Fundamental immunology. USA:Lippincott Willams & Wilkins. 2003:20.
  25. Ivan R et al. Sixth edition Immunology. Spain:Elvevier Science. 2002:119.
  26. 강창희 등. 이공산의 혈관신생 및 암전이 억제효과에 관한 연구. 대한암한의학회지. 2006;11(1):1-21.