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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Recent advances in microfluidic technologies for biochemistry and molecular biology

  • Cho, Soong-Won (Department of Chemistry, Imperial College London) ;
  • Kang, Dong-Ku (Department of Chemistry, Imperial College London) ;
  • Choo, Jae-Bum (Department of Bionano Engineering, Hanyang University) ;
  • Demllo, Andrew J. (Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences) ;
  • Chang, Soo-Ik (Department of Biochemistry, Chungbuk National University)
  • Received : 2011.11.22
  • Published : 2011.11.30
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Abstract

Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large amounts of biological/chemical data into an organized database of knowledge. Microfluidic systems are ideally suited for high-throughput biochemical experimentation since they offer high analytical throughput, consume minute quantities of expensive biological reagents, exhibit superior sensitivity and functionality compared to traditional micro-array techniques and can be integrated within complex experimental work flows. A range of basic biochemical and molecular biological operations have been transferred to chip-based microfluidic formats over the last decade, including gene sequencing, emulsion PCR, immunoassays, electrophoresis, cell-based assays, expression cloning and macromolecule blotting. In this review, we highlight some of the recent advances in the application of microfluidics to biochemistry and molecular biology.

Keywords

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