Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-diabetic Effects of Hemicentrotus pulcherrimus Shells on Non-obese Type 2 Diabetic Goto-Kakizaki Rats

말똥성게(Hemicentrotus pulcherrimus) 껍질 추출물의 Goto-Kakizaki 흰쥐에 대한 항당뇨 효과

  • Kim, Kil-Soo (College of Veterinare Medicine, Kyungpook National University) ;
  • Kim, Dae-Ik (Daegu Technopark Bio Health Convergence Center) ;
  • Lim, Ae-Kyoung (Daegu Technopark Bio Health Convergence Center) ;
  • Yoon, Sung-Ran (Daegu Technopark Bio Health Convergence Center) ;
  • Kim, Jung-Ok (Daegu Gyeongbuk Institute for Oriental Medicine Industry) ;
  • Lee, Gee-Dong (Nutrition Education, Graduate School of Education, Joongbu University)
  • 김길수 (경북대학교 수의과대학) ;
  • 김대익 ((재)대구테크노파크 바이오헬스융합센터) ;
  • 임애경 ((재)대구테크노파크 바이오헬스융합센터) ;
  • 윤성란 ((재)대구테크노파크 바이오헬스융합센터) ;
  • 김정옥 (대구경북한방산업진흥원) ;
  • 이기동 (중부대학교 교육대학원 영양교육)
  • Received : 2011.06.17
  • Accepted : 2011.10.31
  • Published : 2011.11.30
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Abstract

We investigated the anti-diabetic effects of Hemicentrotus pulcherrimus (sea urchin, SU) shells on non-obese type 2 diabetic Goto-Kakizaki (GK) rats. We measured body weight, blood glucose, and plasma insulin levels and conducted an oral glucose tolerance test (OGTT). The SU shells (100 and 200 mg/kg) significantly reduced the blood glucose of GK rats from 203.8${\pm}$29.8 mg/dL to 138.5${\pm}$21.2 mg/dL at after 4 weeks of daily oral administration. However, plasma insulin levels at the same time were not changed by treatment with SU. During the OGTT, the SU-treated GK rats maintained a lower blood glucose level than the control group for 15 to 120 min. Based on these results, SU shells are considered to be effective in improving glucose tolerance. These results suggest that SU shells have unique properties to lower blood glucose, raise insulin sensitivity, and improve insulin resistance in GK rats.

본 연구는 제2형 당뇨동물모델인 Goto-Kakizaki(GK)랫드에 대한 성게껍질의 항당뇨 효과를 조사하였다. 즉 Wistar 랫드로 구성된 정상군, GK랫드 대조군, GK랫드에 성게껍질을 각각 100 mg/kg 및 200 mg/kg 투여군으로 나누고 매일 1회씩 4주간 경구투여 하여 체중, 혈당, 혈장 인슐린 농도 및 경구당부하 검사를 실시하였다. 그 결과 성게껍질을 투여한 GK랫드의 혈당은 203.8${\pm}$29.8 mg/dL에서 138.5${\pm}$21.2 mg/dL로 유의적으로 감소하였다. 그러나 성게껍질투여에 따른 GK랫드의 혈장 내 인슐린 농도의 변화는 없었다. 경구당부하 검사에서 성게껍질을 투여한 GK랫드는 15~120분에서 대조군보다 더 낮은 혈당으로 나타났다. 또한 췌장의 조직학적 변화는 성게껍질을 투여한 랫드에서 전체적인 췌도세포의 크기나 공포성 변화 및 선방세포의 배열 및 크기가 대조군에 비하여 정상군에 근접한 형태로 나타났다. 따라서 성게껍질은 당뇨모델인 GK 랫드의 혈당을 저하시키고 내당능이나 인슐린저항성을 개선하므로 향후 이를 활용하여 항당뇨 식품 및 원료개발에 활용될 것으로 기대된다.

Keywords

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