Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effect of Low Dose γ-Irradiation on the Fate and Cell Envelope of Bacillus cereus, Escherichia coli, and Salmonella Typhimurium

  • Mtenga, Adelard B. (Division of Applied Life Science (BK 21 program), Gyeongsang National University) ;
  • Kassim, Neema (Division of Applied Life Science (BK 21 program), Gyeongsang National University) ;
  • Lee, Won-Gyeong (Division of Applied Life Science (BK 21 program), Gyeongsang National University) ;
  • Heo, Rok-Won (Division of Applied Life Science (BK 21 program), Gyeongsang National University) ;
  • Shim, Won-Bo (The College of Human Sciences, Nutrition, Food and Exercise Sciences, Florida State University) ;
  • Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Chung, Duck-Hwa (Division of Applied Life Science (BK 21 program), Gyeongsang National University)
  • Received : 2011.10.26
  • Accepted : 2011.11.18
  • Published : 2011.12.31
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Abstract

This study investigated the effect of low dose ${\gamma}$-irradiation on the damage of the cell envelopes and antibiotic sensitivity profiles of Bacillus cereus, Escherichia coli, and Salmonella Typhimurium. The bacteria suspension in tryptic soy broth was exposed to the ${\gamma}$-irradiation doses of 0, 1, 1.5, 3, and 5 kGy, and then stored at $0^{\circ}C$ for 24 h. A viability test, an antimicrobial sensitivity profile, and an electron microscopy were performed to observe the effects due to ${\gamma}$-irradiation treatment. B. cereus could survive the ${\gamma}$-irradiation up to 5 kGy while E. coli and S. Typhimurium were all deactivated at 1.5 kGy and 5 kGy, respectively. At 5 kGy, the cell count of B. cereus was significantly reduced, and the survived bacteria cells retained their important features. There were no significant changes observed in the antimicrobial sensitivity profile (p>0.05) for the recovered bacteria after irradiation treatment. Low dose ${\gamma}$-irradiation below 3 kGy was found to be insufficient to achieve decontamination of B. cereus and S. Typhimurium. Cell envelope damage and deactivation of different bacteria did not occur in the same manner; thus, deferent doses of ${\gamma}$-irradiation may be required for deactivation of different bacteria.

Keywords

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