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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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p53 is not necessary for nuclear translocation of GAPDH during NO-induced apoptosis

  • Kim, Jum-Ji (Department of Medical Biotechnology, Soonchunhyang University) ;
  • Lee, Mi-Young (Department of Medical Biotechnology, Soonchunhyang University)
  • Received : 2011.08.09
  • Accepted : 2011.09.16
  • Published : 2011.12.31
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Abstract

Aberrant GAPDH expression following S-nitrosoglutathione (GSNO) treatment was compared in HepG2 cells, which express functional p53, and Hep3B cells, which lack functional p53. The results of Western blotting and fluorescent immunocytochemistry revealed that nuclear translocation and accumulation of GAPDH occur in both HepG2 and Hep3B cells. This finding suggests that p53 may not be necessary for the GSNO-induced translocation of GAPDH to the nucleus during apoptotic cell death in hepatoma cells.

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References

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