Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Inhibitory Effects of Anthocyanins Isolated from Black Soybean (Glycine max L.) Seed Coat on Degranulation and Cytokine Generation in RBL-2H3 Cells

검정콩 껍질 유래 안토시아닌의 RBL-2H3 세포에서 탈과립화와 사이토카인 생성 저해 효과

  • Chung, Mi-Ja (Dept. of Biotechnology, The Catholic University of Korea) ;
  • Ha, Tae-Joung (Dept. of Functional Crop, National Institute of Crop Science (NCIS), Rural Development Administration (RDA)) ;
  • Choi, Ha-Na (Dept. of Biotechnology, The Catholic University of Korea) ;
  • Lee, Ji-Sun (Dept. of Biotechnology, The Catholic University of Korea) ;
  • Park, Yong-Il (Dept. of Biotechnology, The Catholic University of Korea)
  • 정미자 (가톨릭대학교 생명공학과) ;
  • 하태정 (농촌진흥청 국립식량과학원 기능성 작물과) ;
  • 최하나 (가톨릭대학교 생명공학과) ;
  • 이지선 (가톨릭대학교 생명공학과) ;
  • 박용일 (가톨릭대학교 생명공학과)
  • Received : 2011.10.04
  • Accepted : 2011.10.12
  • Published : 2011.12.31
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Abstract

Anthocyanins belong to a group of flavonoid compounds and are well known for their various health beneficial effects, which include antioxidative activities. Among them, the major anthocyanins isolated from seed coat of black soybean (Glycine max L.) were previously characterized as glycosides containing glucopyranose. Asthma is an allergic disease that is strongly associated with various immune cells, including basophils and mast cells. Eosinophils, basophils, and mast cells play important roles in allergic asthma through the release of inflammatory mediators such as asthma-specific T-helper 2 (Th2) cytokines and subsequent amplification of asthma symptoms via degranulation. Rat basophilic leukemia RBL-2H3 cells are the most common in vitro models for evaluating allergic reactions. In this study, we examined the effects of anthocyanin from seed coat of black soybean on antigen-stimulated degranulation and Th2 cytokine production in RBL-2H3 cells. Cell degranulation was evaluated by measuring the release of ${\beta}$-hexosaminidase. ${\beta}$-Hexosaminidase release and Th2 cytokine production in RBL-2H3 cells was much higher upon stimulation with IgE-antigen complex than those in untreated control cells. Anthocyanins significantly suppressed IgE-antigen complex-induced degranulation of RBL-2H3 cells and inhibited IgE-antigen complex-mediated interleukin (IL)-4, IL-13, and tumor necrosis factor ${\alpha}$ (TNF-${\alpha}$) production in RBL-2H3 cells. These findings suggest that anthocyanins from seed coat of black soybean effectively inhibit allergic reactions and may have beneficial effects against allergic asthma.

안토시아닌(anthocyanins)은 플라보노이드에 속하고 항산화 활성들을 포함한 그들의 다양한 건강 유익성에 대해 알려져 있다. 그들 중 검정콩 껍질에서 분리한 주요 안토시아닌은 glycopyranose를 함유하고 있는 배당체이다. 천식은 호염기성 세포(basophils)와 비만세포(mast cells)를 포함한 다양한 면역세포와 관련된 알레르기 관련 질병이다. 호산구(eosinophils), 호염기성 세포, 비만세포는 탈과립화에 의한 천식-특이적 보조 2(T-helper 2) 사이토카인 분비 그리고 계속해서 일어나는 증폭과 같은 염증을 일으키는 매개체의 증가를 통해 알레르기 천식(allergic asthma)에서 중요한 역할을 한다. 흰쥐 호염기성 백혈병(rat basophilic leukemia) RBL-2H3 세포는 알레르기 반응을 측정하기 위해 가장 일반적으로 사용되는 in vitro 모델이다. 본 연구에서는 검정콩 껍질 안토시아닌이 항원으로 자극한 RBL-2H3 세포에서 탈 과립 그리고 Th2 사이토카인 생산에 미치는 효과를 조사하였다. 세포 탈과립은 ${\beta}$-hexosaminidase의 방출을 검출함으로써 평가하였다. IgE-항원 복합체로 자극한 RBL-2H3 세포내 ${\beta}$-hexosaminidase 방출과 Th2 사이토카인 생산이 무처리군의 그것과 비교하여 더 높았다. 안토시아닌은 RBL-2H3 세포의 IgE-항원 복합체 유도 탈과립을 현저하게 억제시켰고 RBL-2H3 세포내 IgE-항원 복합체-매개체 interleukin(IL)-4, IL-13 그리고 TNF-${\alpha}$ 생산을 저해하였다. 이러한 결과는 검정콩 껍질 유래 안토시아닌이 알레르기 반응(allergic reaction)을 현저히 저해하는 효과가 있음을 보였고, 이들 안토시아닌이 향후 알레르기 천식을 억제하거나 개선하는데 유용한 물질로 사용될 가능성이 있을 것으로 판단되었다.

Keywords

References

  1. Fels AO, Cohn ZA. 1986. The alveolar macrophages. J Appl Physiol 60: 355-369.
  2. Larche M, Robinson DS, Kay AB. 2003. The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol 111: 450-463. https://doi.org/10.1067/mai.2003.169
  3. Romagnami S. 2001. T-cell responses in allergy and asthma. Curr Opin Allergy Clin Immunol 1: 73-78. https://doi.org/10.1097/01.all.0000010988.60715.c8
  4. Oshita A, Hamelmann E, Haczku A, Takeda K, Conrad DH, Kikutani H, Gelfand W. 1997. Modulation of antigen-induced B and T cell responses by antigen-specific IgE antibodies. J Immunol 159: 4056-4063.
  5. Lee MY, Seo CS, Lee JA, Lee NH, Kim JH, Ha HK, Zheng MS, Son JK, Shin HK. 2011. Anti-asthmatic effects of Angelica dahurica against ovalbumin-induced airway inflammation via upregulation of heme oxygenase-1. Food Chem Toxicol 49: 829-837. https://doi.org/10.1016/j.fct.2010.12.004
  6. Vo TS, Kong CS, Kim SK. 2011. Inhibitory effects of chitooligosaccharides on degranulation and cytokine generation in rat basophilic leukemia RBL-2H3 cells. Carbohyd Polym 84: 649-655. https://doi.org/10.1016/j.carbpol.2010.12.046
  7. Lee SH, Bae EA, Park EK, Shin YW, Baek NI, Han EJ, Chung HG, Kim DH. 2007. Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princepsin IgE-induced hypersensitivity. Int Immunopharmacol 7: 1678-1684. https://doi.org/10.1016/j.intimp.2007.08.028
  8. Lila MA. 2004. Anthocyanins and human health: an in vitro investigative approach. J Biomed Biotechnol 2004: 306-313. https://doi.org/10.1155/S111072430440401X
  9. Amorini AM, Lazzarino G, Galvano F, Fazzina G, Tavazzi B, Galvano G. 2003. Cyanidin-3-O-beta-glucopyranoside protects myocardium and erythrocytes from oxygen radical- mediated damages. Free Radic Res 37: 453-460. https://doi.org/10.1080/1071576021000055253
  10. Lee JH, Kang NS, Shin SO, Shin SH, Lim SG, Suh DY, Baek IY, Park KY, Ha TJ. 2009. Characterization of anthocyanins in the black soybean (Glycine max L.) by HPLCDAD-ESI/MS analysis. Food Chem 112: 226-231. https://doi.org/10.1016/j.foodchem.2008.05.056
  11. Chung MJ, Sung NJ, Park CS, Kweon DK, Mantovani A, Moon TW, Lee SJ, Park KH. 2008. Antioxidant and hypocholesterolemic activities of water-soluble puerarin glycosides in HepG2 cells and in C57 BL/6J mice. Eur J Pharmacol 578: 159-170. https://doi.org/10.1016/j.ejphar.2007.09.036
  12. Huang F, Yamaki K, Tong X, Fu L, Zhang R, Cai Y, Yanagisawa R, Inoue KI, Takano H, Yoshino S. 2008. Inhibition of the antigen-induced activation of RBL-2H3 cells by sinomenine. Int Immunopharmacol 8: 502-507. https://doi.org/10.1016/j.intimp.2007.12.009
  13. Han EH, Hwang YP, Kim HG, Park JH, Choi JH, Im JH, Khanal T, Park BH, Yang JH, Choi JM, Chun SS, Seo JK, Chung YC, Jeong HG. 2011. Ethyl acetate extract of Psidium guajava inhibits IgE-mediated allergic responses by blocking Fc$\varepsilon$RI signaling. Food Chem Toxicol 49: 100-108. https://doi.org/10.1016/j.fct.2010.10.003
  14. Cerezo AB, Cuevas E, Winterhalter P, Garcia-Parrilla MC, Troncoso AM. 2010. Isolation, identification, and antioxidant activity of anthocyanin compounds in Camarosa strawberry. Food Chem 123: 574-582. https://doi.org/10.1016/j.foodchem.2010.04.073
  15. Astadi IR, Astuti M, Santoso U, Nugraheni PS. 2009. In vitro antioxidant activity of anthocyanins of black soybean seed coat in human low density lipoprotein (LDL). Food Chem 112: 659-663. https://doi.org/10.1016/j.foodchem.2008.06.034
  16. Wendeler M, Sandhoff K. 2009. Hexosaminidase assays. Glycoconjugate J 26: 945-952. https://doi.org/10.1007/s10719-008-9137-5
  17. Matsuda H, Wang Q, Matsuhira K, Nakamura S, Yuan D, Yoshikawa M. 2008. Inhibitory effects of thunberginols A and B isolated from Hydrangeae Dulcis Folium on mRNA expression of cytokines and on activation of activator protein-1 in RBL-2H3 cells. Phytomedicine 15: 177-184. https://doi.org/10.1016/j.phymed.2007.09.010
  18. Kato N, Motohashi S, Okada T, Ozawa T, Mashima K. 2008. PICOT, protein kinase C θ-interacting protein, is a novel regulator of Fc$\varepsilon$RI-mediated mast cell activation. Cell Immunol 251: 62-67. https://doi.org/10.1016/j.cellimm.2008.04.005
  19. Song MY, Jeong GS, Lee HS, Kwon KS, Lee SM, Park JW, Kim YC, Park BH. 2010. Sulfuretin attenuates allergic airway inflammation in mice. Biochem Biophys Res Commun 400: 83-88. https://doi.org/10.1016/j.bbrc.2010.08.014
  20. Choi JH, Hwang YP, Lee HS, Jeong HG. 2009. Inhibitory effect of Platycodi Radix on ovalbumin-induced airway inflammation in a murine model of asthma. Food Chem Toxicol 47: 1272-1279. https://doi.org/10.1016/j.fct.2009.02.022

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