Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Comparison of Enrichment Media of Shigella sonnei

쉬겔라 증균배지의 성능 비교

  • In, Ye-Won (Dept. of Food and Nutrition, Kookmin University) ;
  • Ha, Su-Jeong (Dept. of Food and Nutrition, Kookmin University) ;
  • Kim, Seok-Joong (Dept. of Food and Nutrition, Dongduk Women's University) ;
  • Oh, Se-Wook (Dept. of Food and Nutrition, Kookmin University)
  • 인예원 (국민대학교 식품영양학과) ;
  • 하수정 (국민대학교 식품영양학과) ;
  • 김석중 (동덕여자대학교 식품영양학과) ;
  • 오세욱 (국민대학교 식품영양학과)
  • Received : 2011.08.10
  • Accepted : 2011.11.18
  • Published : 2011.12.31
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Abstract

The object of this study was to compare the performance of commercial enrichment media used for Shigella spp. A total of four enrichment media, Gram negative (GN) broth, Shigella broth (SB), selenite-F (SF) broth, and selenite cystine (SC) broth, were tested. When S. sonnei was inoculated into each enrichment broth at 10 cfu/mL of concentration, the highest growth was observed in Shigella broth. Morganella spp., which was not differentiated in selective agar of Shigella spp. thus can be counted as false positive, did not grow in Shigella broth in enrichment step. When S. sonnei was artificially inoculated into pork, it was mostly recovered through an enrichment process with GN broth and SF broth. However, in the case of beef, S. sonnei was mostly recovered with GN broth but largely failed with Shigella broth. Therefore, enrichment media for Shigella spp. should be selected by considering the food matrix in order to increase the chance of isolating it from foods.

연구는 식품으로부터 Shigella spp.를 분리하기 위해서 현재 사용되고 있는 증균배지를 대상으로 증균성능을 비교하였다. GN broth, Shigella broth, SF broth, SC broth 총 4개의 증균배지를 비교하였다. S. sonnei에 대해 각 접종 수준에 대한 성장은 Shigella broth에서 $10^1$ cfu/mL 낮은 수준의 존재 시에도 성장이 확인되었다. 분리배지에서 거의 구분이 되지 않는 Morganella spp.에 대한 증균은 Shigella broth를 사용할 경우 성장이 저해되었다. 식품을 대상으로 한 S. sonnei의 증균성능은 돼지고기에서 GN broth와 SF broth가 높은 증균능력을 보였으며 소고기에서는 GN broth에서의 증균능력이 높게 나타났으나 Shigella broth에서 증균능력이 높지 않았다. 실험에 사용된 배지는 식품 종류에 따라 증균되는 특성이 상이하므로 식품에서 Shigella spp.를 분리하고자 할 경우 우선적으로 식품 특성을 고려하여 증균배지를 선택하는 것이 바람직할 것으로 생각되었다.

Keywords

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