Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Evaluation of Efficacy and Development of Predictive Model of Sanitizers and Disinfectants on Reduction of Microorganisms on Food Contact Surfaces

스테인리스 스틸 식품기구 표면에 사용되는 주요 살균소독제의 살균력 평가 및 살균예측모델 개발

  • Lee, Yu-Si (School of Food Science and Technology, Chung-Ang University) ;
  • Ha, Sang-Do (School of Food Science and Technology, Chung-Ang University) ;
  • Kim, Dong-Ho (Seowon University) ;
  • Park, Joon-Hee (School of Food Science, College of Science and KyungPook National University)
  • Received : 2011.07.17
  • Accepted : 2011.09.07
  • Published : 2011.09.30
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Abstract

This study was to evaluate the efficacy of sanitizer concentrations and treatment time against two major toad-borne pathogenic microorganisms such as Escherichia coli and Staphylococcus aureus on a stainless steel surface. As a result, stainless steel, treated with 100 ppm of chlorine showed reduction of E. coli(1.56, 1.49, 1.95 log cfu/25 $cm^2$) and S. aureus(0.49, 0.88, 1.27 log cfu/25 $cm^2$) after 0, 5 and 10 min, but none was not detected in treatment with 200 ppm. The population of E. coli(0.73, 0.90, 1.55 log cfu/25 $cm^2$) and S. aureus(0.37, 1.00, 1.45 log cfu/25 $cm^2$) reduced in 35.5% ethanol treated group, but none was not detected in treatment with 70%. The population was reduced E coli(0.28, 0.64, 1.07 cfu/25 $cm^2$) and S. aureus(0.53, 0.87, 0.99 log cfu/25 $cm^2$) by treatment with 45.5 ppm of hydrogen peroxide, but none was not detected in treatment with 91 ppm. Quarternary ammonium compound with 100 ppm was reduced E. coli(0.82, 1.62, 1.71 log cfu/25 $cm^2$) and S. aureus(0.46, 0.93, 1.38 log cfu/25 $cm^2$), but none was not detected in treatment with 200 ppm. Predictive models of sterilization for all 4 disinfectants were suitable to use with $r^2$ value of higher than 0.94. These models may be of use to food services and manufacture of safe products by controlling E. coli and S. aureus without the need for further detection of the organisms.

본 연구는 기구 표면에 존재하는 주요 오염균인 E. coli와 S. aureus에 대한 살균소독제의 농도와 처리 시간별 살균력을 평가하여 살균예측모델 개발 및 살균소독지침의 기초자료로 제공하고자 한다. 염소계 살균소독제 100ppm처리 직후, 5분,\0분 후 E. coli( 1.56, 1.49, 1.95 log cfu/25 $cm^2$)와 S. aureus(0.49, 0.88, 1.27 log cfu/25 $cm^2$)에 대해 높은 감소율을 보였고 200ppm처리 시에는 불검출 되었다. 알콜계 살균소독제는 35.5% 처리 직후, 5분, 10분 후 E. coli(0.73, 0.90. 1.55 log cfu/25 $cm^2$), S. aureus(0.37, 1.00, 1.45 log cfu/25 $cm^2$)에 대해 높은 감소율을 보였고, 70% 5분 처리 시 불검출되었다. 과산화수소계 살균소독제는45.5 ppm 처리 직후. 5분, 10분 후 E. coli(0.28, 0.64, l.07 10g cfu/25 $cm^2$), S. aureus(0.53, 0.87, 0.99 log cfu/25 $cm^2$)에 대해 높은 살균력을 보였으나, 91 ppm 5분 처리 시 검출되지 않았다. 4급암모늄계는 100 ppm 처리 직후, 5분, 10분 후 E. coli(0.82, 1.62. 1.71 log cfu/25 $cm^2$), S. aureus(0.46, 0.93, 1.38 log cfu/25 $cm^2$)에 대한 높은 감소율을 보였으나 200 ppm 5분 처리 시 불검출 되었다. 살균예측모델의 경우, $R^2$ 값이 모두 9.4 이상으로 두 균주 모두 높은 적합성을 보였다. 기구 등의 살균소독제의 사용은 적정한 농도와 처리시간을 준수하여 야만 병원성미생물 감소에 효과적이므로 본 연구에서 개발된 4가지 주요 살균소독제의 살균예측모델을 현장에서 활용함으로써 최적의 살균소독제 처리농도와 처리시간을 추가 실험 없이 시뮬레이션으로 도출 가능할 것이다.

Keywords

References

  1. Wirtanen, G. and Salo, S.: Disinfection in food processing efficacy testing of disinfectants. Rev. Environ. Sci. Biotechnol., 2, 293-306 (2003).
  2. Kim, H. Y., Jeon, D. H., Yoon, H. J., Kwak, I. S., Eom, M. O., Sung, J. H., Park, N. Y., Won, S. A., Bae, S. Y., Lee, Y. J. and Kim, S. H.: Establishing test method of Sporicidal activity of commercial Sterilants. J. Fd. Hyg. Safety, 24, 312-317 (2009).
  3. KFDA, Food code. Korea Food and Drug Administration, Seoul, Korea (2010).
  4. Kwak, T. K. and Moon, H. K.: School food safety practices in USA. J. Korean Diet Assoc., 11, 473-484 (2005)
  5. Kim, J. H., Kim, Y. S. and Han, J. S.: Disinfection state and effective factors of facilities and utilities of elementary school in Busan -Based in the characteristics of dietitian, employee and foodservice-. J. Korean Diet. Assoc., 10, 34-46 (2004).
  6. Gallay, A.: Toxines microbiennes dans les toxi-infections alimentaires collectives declarees en France. Congress of the French Society of Microbiology November Paris (2002)
  7. Mah, T. F. and O'Toole G. A.: Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol., 9, 34-39 (2001). https://doi.org/10.1016/S0966-842X(00)01913-2
  8. Luppens, S. B., Reij, M. W., Heijden, R. W., Rombouts, F. M. and Abee, T.: Development of a standard test to assess the resistance of Staphylococcus aureus biofilm cells to disinfectants. Appl. Environ. Microbiol., 68, 4194-4200 (2002). https://doi.org/10.1128/AEM.68.9.4194-4200.2002
  9. Wilfido, J. B., Artur X, R. S., Manuela, H. H., Tomas, L. P. and Buenaventura, G.: Bactericidal efficacy of peracetic acid in combination with hydrogen peroxide against pathogenic and non pathogenic strains of Staphylococcus spp., Listeria spp. and Escherichia coli. Food Control, 17, 516-521 (2006). https://doi.org/10.1016/j.foodcont.2005.02.014
  10. Yeon, J. H., Kim, I. J., Park, K. H., Park, B. K., Park, H. K., Park, D. W., Kim, Y. S., Kim, H. I., Jeon, D. H., Lee, Y. J. and Ha, S. D.: Treatment and effect of sanitizers and disinfectants in animal food manufacturing plant. Korean J. Food Sci. Technol., 38, 599-603 (2006).
  11. Pertti, P., Pentti, K. and Anna-Lisa, P.: The effectiveness of some disinfectants and detergents against microbial activity. Bldg. Environ., 32, 281-287 (1997). https://doi.org/10.1016/S0360-1323(96)00061-3
  12. 강길진: 식품위생 향상을 위한 살균소독제, 식품과학과 산업, pp. 99-106 (2005).
  13. Grab, L. A. and Bennett, M. K.: Methods of testing sanitizers and bacteriostatic substances. In Disinfection, Sterilization and Preservation, pp. 1373-1382 (2001).
  14. 김용수, 목철균, 하상도, 조영주: 살균소독제 관리방안에 관 한 연구(1), 식품의약품안전청, 한국보건산업진흥원 용역연구사업 연구결과보고서 (2006).
  15. 김용수, 최성희, 하상도: 살균소독제 관리방안에 관한 연구 (2), 식품의약품안전청, 한국보건산업진흥원 용역연구사업 연구결과보고서 (2007).
  16. 학교급식위생관리지침서 제 2판, 교육인적자원부, pp. 39-48 (2004).
  17. Kim, H. I., Park, S. K., Kwak, I. S., Sung, J. H., Lim, H. S., Kim, H. J. and Kim, S. H.: Efficacy of sanitizer due to the change of contact time and temperature. J. Fd Hyg. Safety, 25, 325-332 (2010).
  18. Kim, A. Y., Kim, Y. S. and Ha, S. D.: Comparison study on efficacies of disinfectants and sanitizers among methods for quantitative surface test. J. Fd Hyg. Safety, 25, 238-224 (2010).
  19. Duffy, L. L., Vanderline, P. B. and Grau, F. H.: Growth of Listeria monocytogenes on vaccum-packed cooked meats: effects of pH, Aw, nitrite and sodium ascorbate. Int. J. Food Microbiol. 23, 377-390 (1994). https://doi.org/10.1016/0168-1605(94)90164-3
  20. Peng, J. S., Tsai, W. C. and Chou, C. C.: Inactivation and removal of Bacillus cereus by sanitizer and detergent. Int. J. Food Microbiol., 77, 11-18 (2002). https://doi.org/10.1016/S0168-1605(02)00060-0
  21. Lee, M. J.: Reduction of Bacillus cereus treated with sanitizers and disinfectants. Chung-Ang University. Korea (2006).
  22. Joseph, B., Otta, S. K., Karunasagar, I. and Karunasagar, I.: Biofilm formation by Salmonella spp. on food contact surfaces and their sensitivity to sanitizer. Int. J. Food Microbiol., 64, 367-372 (2001). https://doi.org/10.1016/S0168-1605(00)00466-9
  23. Vianna, M. E., Gomes, B. P., Berber, V. B., Zaia, A. A., Ferraz, C. C. and de Souza-Filho, F. J.: In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. Oral Sung. Oral Med. Oral Pathol. Oral Radiol. Endod., 97, 79-84 (2004). https://doi.org/10.1016/S1079-2104(03)00360-3
  24. Kampf, G., Rudolf, M., Labadie, J. C. and Barrett. S. P.: Spectrum of antimicrobial activity and user acceptability of the hand disinfectant agent sterillium gel. J. Hosp. Infect., 52, 141-147 (2002) https://doi.org/10.1053/jhin.2002.1281
  25. Hernndez, A., Martro, E., Matas, L., Martin, M. and Ausina, V.: Assessment of in-vitro efficacy of 1% virkon against bacteria, fungi, viruses and spores by means of AFNOR guidelines. J. Hosp. Infect., 46, 203-209 (2000).
  26. Lee, M. J., Kim, Y. S., Cho, Y. H., Park, H. K., Park, B. K., Lee, K. H., Kang, K. J., Jeon, D. H., Park, K. H. and Ha, S. D.: Evaluation of efficacy of sanitizers and disinfectants marketed in Korea. Korean J. Food Sci. Technol., 37, 671-677 (2005).