Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Optimized Culture Conditions of Fibrinolytic Enzyme Producing Strain Isolated from Korean Traditional Soybean Sauce

간장 유래 혈전분해 효소 생산 균주의 분리 및 배양학적 특성

  • Baek, Seong-Yeol (Fermentation & Food Processing Division, Department of Agro-food Resource, NAAS, RDA) ;
  • Yun, Hye-Ju (Fermentation & Food Processing Division, Department of Agro-food Resource, NAAS, RDA) ;
  • Park, Heui-Dong (Department of Food Science & Technology, Agro Biotechnology Education Center, Kyungpook National University) ;
  • Yeo, Soo-Hwan (Fermentation & Food Processing Division, Department of Agro-food Resource, NAAS, RDA)
  • 백성열 (농촌진흥청 국립농업과학원 농식품자원부 발효이용과) ;
  • 윤혜주 (농촌진흥청 국립농업과학원 농식품자원부 발효이용과) ;
  • 박희동 (경북대학교 농업생명과학대학 식품공학과) ;
  • 여수환 (농촌진흥청 국립농업과학원 농식품자원부 발효이용과)
  • Received : 2011.10.13
  • Accepted : 2011.11.29
  • Published : 2011.12.28
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Abstract

Bacterial strains exhibiting fibrinolytic activity were screened from traditional Korean soybean sauce. The Fibrinolytic activities of the various isolated microorganism were further examined and the superior strain YJ11-21 was selected for further analyses. Gene sequence analysis of 16S rDNA of the YJ11-21 strain revealed Bacillus licheniformis. Optimal culture conditions were investigated in order to maximize the production of the fibrinolytic enzyme by YJ11-21. Amongst the carbon sources tested, glucose was the most effective for enzyme production and amongst the nitrogen sources tested, yeast extract was seen to be the most effective. A one percent addition of NaCl to the medium resulted in the highest fibrinolytic activity. Interestingly, a 10% addition of NaCl resulted in a high activity together with a high cell growth rate. Therefore, YJ11-21 is speculated of being a halotolerant. The optimum pH and temperature for enzyme production were a pH of 9.0 and $30^{\circ}C$, respectively.

전통발효식품인 재래간장에서 fibrinolytic enzyme를 생산하는 미생물을 분리하였고 산업적으로 활용하기 위해 분리된 미생물 중 fibrinolytic enzyme 활성이 우수한 YJ11-21 균주를 선발하였다. YJ11-21을 분자수준에서 16S rDNA 유전자 염기서열을 분석한 결과, Bacillus licheniformis로 동정되었다. Fibrinolytic enzyme 생산을 위한 최적 배양조건으로서 탄소원은 glucose, 질소원은 yeast extract를 첨가하였을 때 가장 높게 나타났다. 그리고 1% NaCl를 첨가한 배지에서 혈전용해 효소활성이 가장 높게 나타났고, 10% NaCl에서도 높은 효소활성과 생장률 또한 양호하여 YJ11-21는 내염성균으로 추정된다. YJ11-21를 $30^{\circ}C$에서 배양시 가장 높은 효소 활성과, 초발 pH 5-10까지 안정한 효소활성을 보였다. 그리고 pH 9에서 가장 높은 혈전용해 효소활성과 균주의 생장률이 나타났다.

Keywords

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