Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Promoting Effect of Pleuropterus cilinervis Extracts Fermented with Lactobacillus rhamnosus on Hair Growth

백하수오 발효액의 모발성장 효과

  • Park, Jang-Soon (Department of Beauty Science, Kwangju Women's University) ;
  • Lee, Jae-Sug (Department of Beauty Science, Kwangju Women's University)
  • 박장순 (광주여자대학교 미용과학과) ;
  • 이재숙 (광주여자대학교 미용과학과)
  • Received : 2011.05.11
  • Accepted : 2011.09.22
  • Published : 2011.12.28
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Abstract

The bacterial growth and pH of Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus during the fermentation process, were evaluated. The results indicated that the bacterial count after fermentation always remained higher than 6 log CFU/mL, with a constant pH of approximately 4. In order to evaluate the effects of Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus, on hair growth promotion, C57BL/6 mice were chosen as experimental subjects. Six week old males with similar body weights were divided into four groups; a normal group (saline), a negative control group (essence base), a positive control group and an experimental group (Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus, mixed with negative control). The substances and test materials were applied topically on the back skin of the mice for 8 days. Other external conditions and variables such as food intake were kept at the same as the four groups. At the end of the experiment it was noted that hair re-growth in the experimental group, using gross and histological examinations, was higher than that in the positive control group. This study therefore provides an empirical evidence that Pleuropterus cilinervis extracts and animal milk fermented with Lactobacillus rhamnosus promotes hair growth, and suggests that applications could be found in hair loss treatments.

백하수오와 동물성 밀크를 혼합하여 유산균인 Lactobacillus rhamnosus와 함께 1일 동안 배양 한 결과 pH 및 유산균 개체수에 있어서 이상적인 발효에 도달한 것을 알 수 있었다. 이러한 발효물을 생후 6주령 된 C57BL/6 마우스에 1일 1회, 8일 동안 붓을 사용하여 매 회 100 ${\mu}L$씩 경피 도포하여 모발성장 촉진효과를 관찰한 실험에서 다음과 같은 결과를 얻었다. 실험기간 동안 모든 군에서 체중과 사료 섭취량이 큰 차이를 나타내지 않았으며, 육안적 관찰에서 무처리군과 음성대조군에서 모발성장이 약하게 관찰되었고, 양성대조군인 미녹시딜 처리군에서는 평균 62%의 모발성장이 관찰되었으며, 실험군인 백하수오 유산균 발효군에서는 평균 90% 이상의 정상적인 모발성장이 관찰되었다. 피부조직의 H&E 염색 후 분석 결과 8일째 희생한 마우스의 무처리군과 음성 대조군에서 모낭의 수 및 크기가 작고 모근의 길이가 작았고, 양성대조군에서는 모낭의 수, 크기 및 모근의 길이가 무처리군과 음성대조군에 비해 약간 신장한 것을 알 수 있었으며, 백하수오 유산균 발효군인 실험군에서는 모낭의 수, 크기 및 모근의 길이가 다른 군에 비해 크게 관찰되었다. 이상의 실험결과를 통해 백하수오와 동물성 밀크를 유산균인 Lactobacillus rhamnosus와 같이 발효하여 얻은 발효액은 우수한 모발성장 촉진효과를 보였으며, 미녹시딜보다 더 높은 효과를 나타냄을 알 수 있었다.

Keywords

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