Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Expression of a bpr Gene Encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1

  • Kwon, Gun-Hee (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University) ;
  • Park, Jae-Yong (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Kim, Jong-Sang (School of Applied Biosciences, Kyungpook National University) ;
  • Lim, Jin-Kyu (School of Applied Biosciences, Kyungpook National University) ;
  • Park, Cheon-Seok (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute) ;
  • Kim, Jeong-Hwan (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
  • Received : 2010.10.27
  • Accepted : 2011.02.12
  • Published : 2011.05.28
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Abstract

A gene encoding bacillopeptidase F, bpr86-1, was cloned from B. amyloliquefaciens CH86-1 isolated from cheonggukjang. This gene could encode a preproenzyme of 1,431 amino acids. When bpr86-1 was introduced into B. subtilis WB600 via pHY300PLK, an E. coli-Bacillus shuttle vector, the transformant showed fibrinolytic activity. During growth on LB, the fibrinolytic activity of cells increased sharply when they entered the stationary phase. The highest activity (761.4 mU/mg protein) was observed at 96 h of cultivation.

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References

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