Phylogenetic Analysis of Sorangium cellulosum Strains Based on Cellulase Gene Sequences

Cellulase 유전자 염기서열에 기초한 Sorangium cellulosum 균주들의 계통분류

  • Lee, Han-Bit (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Youn, Jin-Kwon (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Cho, Kyung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
  • 이한빛 (호서대학교 생명공학과 점액세균은행) ;
  • 윤진권 (호서대학교 생명공학과 점액세균은행) ;
  • 조경연 (호서대학교 생명공학과 점액세균은행)
  • Received : 2010.11.11
  • Accepted : 2010.12.08
  • Published : 2011.03.28

Abstract

Phylogenetic analysis of two cellulase genes, xynB1 and bglA2, and the groEL1 gene from 34 Sorangium cellulosum strains isolated in Korea suggested that there are at least five subgroups in S. cellulosum, which is the most proficient producer of secondary metabolites among myxobacteria. This analysis also revealed diversity among the isolated S. cellulosum. It appeared that at least 30 out of 34 strains are different each other.

두 개의 cellulase 유전자 xynB1, bglA2과 groEL1 유전자 염기서열에 기초하여 국내에서 분리한 34균주의 Sorangium cellulosum 균주들을 계통 분석한 결과, 점액세균 중 가장 많은 생리활성물질이 발견된 종인 S. cellulosum 내에는 최소한 5그룹의 소그룹이 존재함을 보였다. 이 분석은 또한 S. cellulosum 균주들의 다양성을 보여주어 분석한 34균주 중 30균주가 서로 다른 균주인 것으로 나타났다.

Keywords

References

  1. Gerth, K., N. Bedorf, G. Hofle, H. Irschik, and H. Reichenbach. 1996. Epothilons A and B: antifungal and cytotoxic compounds from Sorangium cellulosum (myxobacteria). Production, physico-chemical and biological properties. J. Antibiot. 49: 560-563. https://doi.org/10.7164/antibiotics.49.560
  2. Gerth, K., N. Bedorf, H. Irschik, G. Hofle, and H. Reichenbach. 1994. The soraphens: a family of novel antifungal compounds from Sorangium cellulosum (myxobacteria). J. Antibiot. 47: 23-31. https://doi.org/10.7164/antibiotics.47.23
  3. Gerth, K., S. Pradella, O. Perlova, S. Beyer, and R. Müller. 2003. Myxobacteria: proficient producers of novel natural products with carious biological activities-past and future biotechnological aspects with the focus on the genus Sorangium. J. Biotech. 106: 233-253. https://doi.org/10.1016/j.jbiotec.2003.07.015
  4. Hyun, H., J. Chung, H. Lee, J. Youn, C. Lee, D. Kim, and K. Cho. 2009. Isolation of cellulose-degrading myxobacteria Sorangium cellulosum. Kor. J. Microbiol. 45: 48-53.
  5. Irschik, H., H. Augustiniak, K. Gerth, G. Hofle, and H. Reichenbach. 1995. The ripostatins, novel inhibitors of eubacterial RNA polymerase isolated from myxobacteria. J. Antibiot. 48: 787-792. https://doi.org/10.7164/antibiotics.48.787
  6. Jansen, R., H. Irschik, V. Huch, D. Schummer, H. Steinmetz, M. Bock, T. Schmidt, A. Kirschning, and R. Müller. 2010. Carolacton - a macrolide ketocarbonic acid that reduces biofilm formation by the caries- and endocarditis-associated bacterium Streptococcus mutans. Eur. J. Org. Chem. 2010: 1284-1289.
  7. Jiang, D. M., L. Zhao, C. Y. Zhang, J. Li, Z. J. Xia, J. Wang, Z. H. Wu, and Y. Z. Li. 2008. Taxonomic analysis of Sorangium strains based on HSP60 and 16S rRNA gene sequences and morphology. Int. J. Syst. Evol. Microbiol. 58: 2654-2659. https://doi.org/10.1099/ijs.0.65806-0
  8. Kunze, B., M. Reck, A. Dotsch, A. Lemme, D. Schummer, H. Irschik, H. Steinmetz, and I. Wagner-Döbler. 2010. Damage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosum. BMC Microbiol. 10: 199. https://doi.org/10.1186/1471-2180-10-199
  9. Krug, D., G. Zurek, O. Revermann, M. Vos, G. J. Velicer, and R. Muller. 2008. Discovering the hidden secondary metabolome of Myxococcus xanthus: a study of intraspecific diversity. Appl Environ Microbiol. 74: 3058-3068. https://doi.org/10.1128/AEM.02863-07
  10. Larkin, M. A., G. Blackshields, N. P. Brown, R. Chenna, P. A. McGettigan, H. McWilliam, F. Valentin, I. M. Wallace, A. Wilm, R. Lopez, J. D. Thompson, T. J. Gibson, and D. G. Higgins. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948. https://doi.org/10.1093/bioinformatics/btm404
  11. Mukhopadhyay, J., K. Das, S. Ismail, D. Koppstein, M. Jang, B. Hudson, S. Sarafianos, S. Tuske, J. Patel, R. Jansen, H. Irschik, E. Arnold, and R. H. Ebright. 2008. The RNA polymerase "switch region" is a target for inhibitors. Cell 135: 295-307. https://doi.org/10.1016/j.cell.2008.09.033
  12. Page, R. D. 1996. TreeView: an application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 12: 357-358.
  13. Reichenbach, H. 2005. Order VIII. Myxococcales, pp. 1059- 1144. In D. J. Brenner, N. R. Krieg, J. T. Staley, and G. M. Garrity (ed.), Bergey's Manual of Systematic Bacteriology, 2nd ed. Bergey's Manual Trust, East Lansing, MI, USA.
  14. Reichenbach, H. and G. Hofle. 1999. Myxobacteria as producers of secondary metabolites, pp. 149-179. In S. Grabley and R. Thiericke (ed.), Drug discovery from nature. Springer, Berlin, Germany.
  15. Schneiker, S., O. Perlova, O. Kaiser, K. Gerth, A. Alici, M. O. Altmeyer, D. Bartels, T. Bekel, S. Beyer, E. Bode, H. B. Bode, C. J. Bolten, J. V. Choudhuri, S. Doss, Y. A. Elnakady, B. Frank, L. Gaigalat, A. Goesmann, C. Groeger, F. Gross, L. Jelsbak, L. Jelsbak, J. Kalinowski, C. Kegler, T. Knauber, S. Konietzny, M. Kopp, L. Krause, D. Krug, B. Linke, T. Mahmud, R. Martinez-Arias, A. C. McHardy, M. Merai, F. Meyer, S. Mormann, J. Muñoz-Dorado, J. Perez, S. Pradella, S. Rachid, G. Raddatz, F. Rosenau, C. Rückert, F. Sasse, M. Scharfe, S. C. Schuster, G. Suen, A. Treuner- Lange, G. Velicer, F. J. Vorhölter, K. J. Weissman, R. D. Welch, S. C. Wenzel, D. E. Whitworth, S. Wilhelm, C. Wittmann, H. Blöcker, A. Pühler, and R. Müller. 2007. Complete genome sequence of the myxobacterium Sorangium cellulosum. Nat. Biotechnol. 25: 1281-1289. https://doi.org/10.1038/nbt1354
  16. Stein, A. 2010. Ixabepilone. Clin. J. Oncol. Nurs. 14: 65-71. https://doi.org/10.1188/10.CJON.65-71
  17. Weissman, K. J. and R. Muller. 2009. A brief tour of myxobacterial secondary metabolism. Bioorg. Med. Chem. 17: 2121-2136. https://doi.org/10.1016/j.bmc.2008.11.025
  18. Wenzel, S. C. and R. Muller. 2009. The impact of genomics on the exploitation of the myxobacterial secondary metabolome. Nat. Prod. Rep. 26: 1385-1407. https://doi.org/10.1039/b817073h