Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of a Cellulase Producing Marine Bacillus sp. GC-1 and GC-4 Isolated from Coastal Seawater of Jeju Island

제주 연안의 해수로부터 분리한 Cellulase 생산균 Bacillus sp. GC-1과 GC-4의 동정

  • Chi, Won-Jae (Department of Biological Science, Myongji University) ;
  • Park, Da-Yeon (Department of Biological Science, Myongji University) ;
  • Temuujin, Uyangaa (Department of Biological Science, Myongji University) ;
  • Lee, Jong-Yeol (National Academy of Agricultural Science, Rural Development Administration) ;
  • Chang, Yong-Keun (Dept. of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Hong, Soon-Kwang (Department of Biological Science, Myongji University)
  • 지원재 (명지대학교 생명과학정보학부) ;
  • 박다연 (명지대학교 생명과학정보학부) ;
  • ;
  • 이종열 (농촌진흥청 국립농업과학원) ;
  • 장용근 (한국과학기술원 생명화학공학과) ;
  • 홍순광 (명지대학교 생명과학정보학부)
  • Received : 2011.02.01
  • Accepted : 2011.03.28
  • Published : 2011.06.28
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Abstract

Two Gram positive bacterial strains, designated strain GC-1 and GC-4, were isolated from coastal seawater near Jeju Island in the Republic of Korea. The two strains were identified as members of the genus Bacillus, based on 16S rRNA gene sequencing and data for physiological characteristics analyses. A subtle difference in physiological and genotypical characteristics has led us to designate the strains GC-1 and GC-4. The strain GC-1 showed a 99.91% similarity in 16S rRNA gene sequencing with B. tequiliensis and B. subtilis subsp. inaquosorum and the strain GC-4 showed a 100% similarity in 16S rRNA gene sequencing with those of B. altitudinis, B. stratosphericus, and B. aerophilus. However, both strains exhibited different physiological and genotypical characteristics in many aspects from those of their phylogenetically closest neighbors listed above, which implies that genus Bacillus has diversified into various species during its evolutionary process.

GC-1과 GC-4로 명명된 두 종의 그람 양성 박테리아가 제주도 연안해수로부터 동정되었다. 이 두 균주는 16S rRNA 유전자 염기서열 분석과 생리적 특성 분석결과를 토대로 Bacillus 속의 박테리아로 규명되었다. 균주 GC-1의 16S rRNA 유전자 염기서열은 B. tequiliensis와 B. subtilis subsp. inaquosorum의 16S rRNA 유전자 염기서열과 99.91%의 상동성을 보였고, 균주 GC-4의 16S rRNA 유전자 염기서열은 B. altitudinis, B. stratosphericus 및 B. aerophilus의 16S rRNA 유전자 염기서열과 100%의 상동성을 보였다. 그러나 두 균주의 생리학적-유전학적 특성 분석 결과, 이들이 계통적 유연관계를 갖는 다른 Bacillus 속의 균주들과 상당한 차이가 있었고, 따라서 조사된 Bacillus 속과는 다른 속에 속할 가능성이 높았다. 이러한 결과는 Bacillus 속이 진화과정 중에 다양한 변종으로 진화되었음을 암시한다.

Keywords

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