한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제39권3호
  • /
  • Pages.281-286
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  • 2011
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

발효 새우젓의 에탄올 추출물이 고지방 식이를 급여한 흰쥐의 콜레스테롤 저하 효과

Cholesterol Lowering Effect of Ethanol Extracts of Salted and Fermented Small Shrimp in Rats Administered a High Fat Diet

  • 김용현 (순천향대학교 생명과학과) ;
  • 한국일 (순천향대학교 생명과학과) ;
  • 전미애 (순천향대학교 생명과학과) ;
  • 권현정 (순천향대학교 생명과학과) ;
  • 박민경 (청운대학교 식품영양학과) ;
  • 한만덕 (순천향대학교 생명과학과)
  • Kim, Yong-Hyun (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Han, Kook-Il (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Jeon, Mi-Ae (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Kwon, Hyun-Jung (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Park, Min-Kyung (Dept. of Human Nutrition & Food Science, College of Engineering & Applied Sciences, Chungwoon University) ;
  • Han, Man-Deuk (Dept. of Biology, College of Natural Science, Soonchunhyang University)
  • 투고 : 2011.05.16
  • 심사 : 2011.06.22
  • 발행 : 2011.09.28
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초록

발효새우젓의 에탄올 추출물이 지질대사 개선 효능을 알아보기 위하여 흰쥐에 고지방사료를 식이 시키고 추출물의 경구투여 시킨 후, 혈중 지질 관련 물질의 변화를 측정하였다. 혈청 총 콜레스테롤 함량은 고지방 식이를 급여한 HC, HC-SFSL, HC-SFSH, HC-Lov군 모두 7일차, 14일차에 대조군(NC) 보다 유의하게 증가하였다. HC-SFSH군은 7일차에 330 mg/dl, 14일차에는 277 mg/dl로 HC군에 비하여 31.5%, 40.4%의 유의한 저하효과가 관찰되었으며 양성 대조군인 lovastatin보다 감소율 대비 12.6배 높은 혈중 총 콜레스테롤 저하 효과를 나타내었다. 혈중 TG 함량은 7일차에 HC-SFSL군, HC-SFSH군 그리고 HC-Lov군에서 62 mg/dl, 30 mg/dl, 55 mg/dl로 HC군 85 mg/dl에 비해 각각 27%, 64.7%, 35.2%의 유의성 있게 감소하였다. 혈중 LDL-콜레스테롤 함량은 HC-SFSH군에서 고지방만 급여한 HC군 보다 7일차에 22.4%, 14일차에 36.3% 감소 하였으며 양성 대조군인 lovastatin 투여군 보다 혈중 LDL-콜레스테롤 수치를 2.4~3.2배 감소시키는 것으로 나타났다. 고지방 및 새우젓 에탄올 추출물을 14일간 투여 후 간의 조직학적 관찰을 한 결과, 일반식이를 급여한 NC군의 간조직 세포는 간정맥을 중심을 향하여 규칙적 배열을 이루고 있었으며, 간세포의 핵은 중심에 위치하고 세포질은 균질된 구조였다. 반면 고지방 식이만 급여한 HC군의 간 조직은 간조직의 지방입자가 전체적으로 지방구를 형성하며 세포핵이 한쪽으로 치우쳐져 있으며 세포질도 많이 파열되어 전형적인 지방간 형태를 나타내었다. 특히 HC-SFSH군의 간조직은 HC군에 비하여 지방구의 숫자와 세포질의 파열정도가 뚜렷하게 감소된 것이 관찰되었다.

This study was performed to investigate the effects of salted and fermented shrimp ethanol extract (SFS) on serum lipid metabolism and hepatocytes in rats. Male Sprague-Dawley rats were administered 60% fat feed to induce hypercholesterolemia and were divided into five groups. Experimental groups were classified according to administered diet: normal diet group (NC), high cholesterol diet group (HC), high cholesterol and low dose shrimp extract (20 mg/kg) group (HC-SFSL), high cholesterol and high dose shrimp extract (200 mg/kg) group (HC-SFSH), and high cholesterol and lovastatin (20 mg/kg) group (HC-Lov). The experimental diets were fed ad libitum for 14 days. Compared with the control group, the serum cholesterol and triglycerides were 40.4 and 64.7% lower in the group fed HC-SFSH respectively. Low density lipoprotein (LDL)-cholesterol concentration in serum decreased in the HC-SFSH group compared with the HC group. In a histological assay, hepatocytes in the HC group showed that the vacuolated cells by fat appear clear due to the large amount of intracytoplasmic fat, whereas the liver hepatocytes in the group fed SFS effectively decreased fatty liver and intracytoplasmic fats. These results suggest that the extract of salted and fermented shrimp has an antiatherosclerotic effect and may lessen the effects of cardiovascular disease by reducing the cholesterol level in serum.

키워드

참고문헌

  1. Barnes, P. M., B. Bloom, and R. L. Nahin. 2007. Complementary and alternative medicine use among adults and children: United States. Natl. Health Stat. Rep. 10(12): 1-23.
  2. Bazzano, L. A., J. He, L. G. Ogden, C. M. Loria, and P. K. Whelton. 2003. Dietary fiber intake and reduced risk of coronary heart disease in US men and women: the National health and nutrition examination survey I epidemiologic follow-up study. Arch. Intern. Med. 163: 1897-1904. https://doi.org/10.1001/archinte.163.16.1897
  3. Carol, A. S. and D. B. Christopher. 1993. Triglycerides and disease. Postgrad. Med. J. 69: 679-695. https://doi.org/10.1136/pgmj.69.815.679
  4. Childs, M. T., C. S. Dorsett, I. B. King, J. G. Ostrander, and W. K. Yamanaka. 1990. Effects of shellfish consumption on lipoproteins in normolipidemic men. Am. J. Clin. Nutr. 51: 1020-1027.
  5. Kim, D. W., D. H. Yang, S. Y. Kim, K. S. Kim, M. G. Chung, and S. M. Kang. 2009. Hypocholesterolemic effect of lyophilized, heat-killed Lactobacillus rhamnosus and Lactobacillus plantarum. Kor. J. Microbiol. Biotechnol. 37(1): 69-74.
  6. Kim, S. J., J. Ma, and L. Kim. 2005. Probiotic properties of actic acid bacteria and yeasts isolated from Korean traditional food, Jeot-gal. Kor. J. Food. Preserv. 12: 84-189.
  7. Lichtenstein, A. H., L. J. Appel, M. Brands, M. Carnethon, S. Daniels, and H. A. Franch. 2006. Diet and lifestyle recommendations revision 2006. Circulation. 114: 82-96. https://doi.org/10.1161/CIRCULATIONAHA.106.176158
  8. Liu, S., M. J. Stampfer, and F. B. Hu. 1999. Whole-grain consumption and risk of coronary heart disease: results from the Nurses Health Study. Am. J. Clin. Nutr. 70: 412-419.
  9. Mensink, R. P., and M. B. Katan. 1992. Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler. Thromb. 12: 911-919. https://doi.org/10.1161/01.ATV.12.8.911
  10. Park, K. Y. 2002. Korean traditional food and their anticancer effects. J. Kor. Soc. Plant. People Environ. 5: 41-45.
  11. Park, S. Y., Y. T. Ko, H. K. Jeong, J. O. Yang, H. S. Chung, Y. B. Kim, and G. E. Ji. 1996. Effect of various lactic acid bacteria on the serum cholesterol levels in rats and resistance to acid, bile and antibiotics. Kor. J. Appl. Microbiol. Biotechnol. 24: 304-310.
  12. Prabhjot, S. N., M. B. Frances, B. Annette, and J. R. Daniel. 2010. Role of dietary supplements in lowering low-density lipoprotein cholesterol: A review. J. Clin. Lipidol. 4: 248- 258. https://doi.org/10.1016/j.jacl.2010.07.001
  13. Rosamond, W., K. Flegal, K. Furie, A. Go, K. Greenlund, and N. Haase. 2008. Heart disease and stroke statistics. Circulation. 117: 25-146.
  14. Saunderson, C. L. and J. Mackinlay. 1990. Changes in body weight, composition and hepatic enzyme activities in response to dietary methionine, betaine and choline levels in growing chicks. British J. Nutr. 63: 339-349. https://doi.org/10.1079/BJN19900120
  15. Seok, S. H., J. H. Park, S. A. Choa, S. A. Choi, and J. H. Park. 2004. Cholesterol lowering effect of SG-GN3, the extract of salted and fermented small shrimps, Acetes japonicus, in Triton WR-1339 or high cholesterol-diet induced hypercholesterolemic rats. J. Ethnopharmacol. 91: 231-235. https://doi.org/10.1016/j.jep.2003.12.032
  16. Sugiyama, K., H. Akai, and K. Muramatsu. 1986. Effect of methionine and related compound on plasma cholesterol level in rats fed high cholesterol diet. J. Nutr. Sci. Vitaminol. 32: 537-549. https://doi.org/10.3177/jnsv.32.537
  17. Tanaka, K., T. Sakai, I. Ikeda, K. Imaizumi, and M. Sugano. 1998. Effects of dietary Shrimp, squid and octopus on serum and liver lipid levels in mice. Biosci. Biotechnol. Biochem. 62(7): 1369-1375. https://doi.org/10.1271/bbb.62.1369
  18. Virtanen, E. 1992. Betain liefert effektiv Methyl gruppen. Kraftfutter. 6: 61-262.