한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제39권3호
  • /
  • Pages.313-316
  • /
  • 2011
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

김치에서 분리한 Lactobacillus sakei proBio65의 면역 조절 특성

Characteristics of immunomodulation by a Lactobacillus sakei proBio65 isolated from Kimchi

  • Lim, Jeong-Heui (Department of Applied Microbiology and Biotechnology, Yeungnam University) ;
  • Seo, Byoung-Joo (Department of Applied Microbiology and Biotechnology, Yeungnam University) ;
  • Kim, Jung-Eun (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Chae, Chang-Suk (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Im, Sin-Hyeog (Department of Pediatrics, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Hahn, Youn-Soo (Department of Pediatrics, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Park, Yong-Ha (Department of Applied Microbiology and Biotechnology, Yeungnam University)
  • 투고 : 2011.08.12
  • 심사 : 2011.09.06
  • 발행 : 2011.09.28
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초록

김치로부터 새로운 프로바이오틱균주인 Lactobacillus sakei proBio65를 분리하고 명명하였다. 형질전환 생쥐(Foxp3-GFP KI 생쥐)를 이용하여 L. sakei proBio65의 면역조절 메커니즘 규명 및 면역 조절능을 확인하고 in vivo 적용 질환제어 응용 가능성을 평가하였다. 조절 T 세포의 master 전사조절인자로 알려진 $Foxp3^+$를 선정하고, L. sakei가 $Foxp3^+$ 전사조절인자를 증가시키는지 확인하기 위해 확립된 세포기반 screening system을 이용하였다. 항 염증성 사이토카인 전사조질인자의 증가에 이어 $Foxp3^+$ 전자조절인자발현의 상당한 증가를 확인하였다. L. sakei proBio65는 염증성면역 장애의 조절에 치료적으로 유용할 것이다.

We isolated and identified a novel probiotic strain, Lactobacillus sakei proBio65 from Kimchi. To determine whether L. sakei proBio65 has an immunomodulatory effect, we investigated cells via an in vitro screening system which co-cultured freshly isolated mesenteric lymphocyte with probiotics. A significant increase of $Foxp3^+$ transcription regulatory factor expression was observed, followed by an increase in anti-inflammatory cytokines transcription regulatory factor. L. sakei proBio65 exhibited high levels of the IL-10/IL-12 production ratio and enhanced Foxp3 expression in vitro. L. sakei proBio65 may thus be therapeutically useful for the modulation of inflammatory immune disorders.

키워드

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