DOI QR코드

DOI QR Code

Changes in Properties of Deer Antler by Proteolysis and Extraction Conditions

녹용의 단백질가수분해 및 추출조건에 따른 특성 변화

  • Received : 2010.07.06
  • Accepted : 2010.12.03
  • Published : 2011.01.31

Abstract

This study was conducted to investigate the proteolysis and extraction conditions of deer antler for application of food materials. ProteAX (A) was the most effective enzyme for proteolysis of deer antler and the proteolysis condition was 0.5% (w/w) for enzyme concentration and 5 hr for proteolysis time. The effect of mixing enzyme ProteAX (A)+KFEN 2 (C) treatment in $60^{\circ}C$, 5 hr was investigated; soluble solid and protein content were the highest with A 0.5% (w/w) and B 0.5% (w/w) concentration. Result for DAH (deer antler hydrolysate) and DA (deer antler) prepared with extraction in $95^{\circ}C$ atmospheric pressure (AP, 6~18 hr) and extraction under $120^{\circ}C$ pressure condition (UP, 15~60 min) after hydrolysis on preceding established condition descriptions indicated that difference in pH according to enzyme treatment and extraction conditions was not significant. Sugar content of DA was $1.5^{\circ}Brix$, DA-UP (under pressure) and DAH-AP (atmospheric pressure) were $2.2^{\circ}Brix$; the highest sugar content of $2.7^{\circ}Brix$ was observed in DAH-UP for 60 min extraction. Also total free sugar, crude protein and collagen content were the highest in DAH-UP for 60 min recording at 1.97%, 742.7 mg/100 g and 498.8 mg/100 g, respectively. From these results, deer antler hydrolysate prepared with extraction under pressure was the most effective for functional characteristics enhancement. Hereafter, various practical uses of materials with enhanced characteristics of antler is expected.

녹용을 식품소재로 활용하고자 단백질 가수분해조건 설정 및 추출조건에 따른 품질특성을 조사하였다. 효소제 종류에 따른 영향을 조사한 결과 효소제 종류에 따른 pH는 차이는 없었으나, $^{\circ}Brix$, 고형분 및 조단백질 함량은 ProteAX(A)에서 높게 나타났다. 효소제 A 농도에서는 0.5%(w/w)까지 $^{\circ}Brix$, 고형분 및 조단백질 함량은 증가하였으며, 1.0%(w/w) 이상에서는 큰 변화가 없었다. 가수분해 시간은 5시간까지 $^{\circ}Brix$, 고형분 및 조단백질 함량은 증가하였으나 그 후 큰 변화가 없어 가수분해 시간을 5시간으로 설정하였다. 녹용에 효소 A와 C를 각각 0.5%(w/w)씩 혼합첨가 하여 $60^{\circ}C$에서 5시간 가수분해할 때 고형분 및 단백질 함량이 가장 높게 나타났다. 상기 설정된 조건으로 가수분해 시킨 녹용가수분해물(DAH)과 대조구(DA)를 $95^{\circ}C$ 상압조건(AP, 6~18시간) 및 $120^{\circ}C$ 가압조건(UP, 15~60분)으로 각각 추출한 결과 pH는 효소처리 및 추출조건에 따른 큰 차이는 없었다. $^{\circ}Brix$는 DA $1.5^{\circ}Brix$, DA-UP 및 DAH-AP에서는 $2.2^{\circ}Brix$로 나타났으며, DAH-UP에서 60분 추출하였을 때 $2.7^{\circ}Brix$로 가장 높게 나타났다. 또한 DAH-UP에서는 가용성 고형분 1.97%, 조단백질 함량 742.7 mg/100 g, 콜라겐 함량 498.8 mg/100 g으로 가장 높게 나타났다. 이상의 결과 가압추출조건이 녹용의 품질특성 향상에 더 효과적인 것으로 나타났으며, 향후 다양한 소재로 활용이 기대되었다.

Keywords

References

  1. Qi SB, Zhao XH, Yang XW, Hattori M, Namba T, Nomura Y. 1988. Effect of ethanol extract of Rokujo, pilose antler, on uptake and release of noradrenal in cerebral cortical slices of rats. J Med Pharm Soc For Wakanyaku 5: 93-97.
  2. Wang BX, Zhao XH, Yang XY, Kaneko S, Hattori M, Namba T, Nomura Y. 2001. Inhibition of lipid peroxidation by deer antler (Rokujo) extract in vivo and in vitro. J Med Pharm Soc For Wakanyaku 5: 123-128.
  3. Shin KH, Lim SS, Lee SH, Lee YS, Cho SY. 2001. Antioxidant and immunostimulating activities of the fruiting bodies of Paecilomyces japonica, a new type of Cordyceps sp. Ann N Y Acad Sci 928: 261-273.
  4. Kim YT, Kim CS. 1996. A study of hematopoietic action of pilose antler in senescence accelerated mice. Kor J Pharmacogn 27: 371-377.
  5. Sin GH. 2002. Deer activity and the active ingredients of the medication. The first KNRC Nokyong Science Symposium-Scientific understanding of deer. Cheongju, Korea. p 55-87.
  6. Kwak HB, Lee SW, Li YJ, Kim YA, Han SY, Jhon GJ, Kim HH, Lee ZH. 2004. Inhibition of osteoclast differentiation and bone resorption by a novel lysophosphatidylcholine derivative, SCOH. Biochem Pharmac 67: 1239-1248. https://doi.org/10.1016/j.bcp.2003.10.032
  7. Shin KH, Lim EB, Kim JH, Chung MS, Cho SI. 1989. Pharmacological studies on powered whole part of unossified antler. Kor J Pharmcogn 20: 180-187.
  8. Park PJ, Jeon YJ, Moon HS, Jeon TB. 2005. Chemical composition and biological activity of velvet antler. Food Industry and Nutrition 10: 50-59.
  9. Lee BY, Lee OH, Choi HS. 2003. Analysis of food components of Korean deer antler parts. Korean J Food Sci Technol 35: 52-56.
  10. Kim DH, Han SB, Park JS, Han MJ. 1994. Fermentation of antler and its biological activity. Kor J Pharmacogn 25: 233-237.
  11. Choi KH, Um CI. 1994. Process for preparing antler extracts. Korean patent 2011.
  12. Lee DS. 2008. Method of preparing deer velvet extract. Korean patent 10568.
  13. Jeon KJ, Woo YJ, Kim JK. 2002. Extract of deer antler containing bone formation enhancing activity and its purifying method. Korean patent 6876.
  14. Baek IB. 2002. A process for preparing antler powder. Korean patent 1557.
  15. Kim MK, Jung EY, Lee HS, Shin KS, Kim YK, Ra KS, Park CS, Woo MJ, Lee SH, Kim JS, Suh HJ. 2009. Isolation of strain for the preparation of the fermented antler and its physiological activities. J Korean Soc Food Sci Nutr 38: 1237-1242. https://doi.org/10.3746/jkfn.2009.38.9.1237
  16. Ann YG. 2004. Extraction of young antler and antler by water, proteases and HCl. Korean J Food & Nutr 17: 147-155.
  17. Lee WB. 2007. Study on the free radical scavenging activity of enzymatic extracts from velvet antler of elk (Cervus Canadensis). MS Thesis. Konkuk University, Seoul, Korea. p 1-3.
  18. Yeo IH, Lee SY, Park JM. 2002. The process method of concentrated powder for enzyme treatment in Cervi Parvum Corun. Korean patent 14395.
  19. An JH. 2002. Hydrolysis manufacture method of buck's cornu. Korean patent 65197.
  20. Gu YA, Jang SY, Park NY, Mun CR, Kin OM, Jeong YJ. 2006. Property changes of mung bean depending on hydrolysis conditions. Korean J Food Preserv 13: 563-568.
  21. Kim KY, Shin JK, Lee SW, Yoon SR, Chung JS, Jeong YJ, Choi MS, Lee CM, Moon KD, Kwon JH. 2007. Quality and functional properties of red ginseng prepared with different steaming time and drying methods. Korean J Food Sci Technol 39: 494-499.
  22. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-271.
  23. Kolar K. 1990. Colorimetric determination of hydroxyproline as measure of collagen content in meat and products. NMKL Collaborative Study. J Assoc Off Anal Chem 73: 55-60.
  24. Lee SH, Cho YJ, Kim S, Ahn BJ, Choi C. 1995. Optimal conditions for the enzymatic hydrolysis of isolated sesame meal protein. J Appl Biol Chem 38: 248-253.
  25. Ann YG. 2003. Extraction of freeze dried young antler by water and protease. Korean J Food & Nutr 16: 379-387.
  26. Pommer K. 1995. New proteolytic enzymes for the production of savory ingredients. Cereal Foods World 40: 745-748.

Cited by

  1. Physicochemical and Sensory Characteristics of Beef-bone Broths Prepared under Atmospheric Pressure and Overpressure vol.47, pp.6, 2015, https://doi.org/10.9721/KJFST.2015.47.6.725
  2. Characteristics of protein from red crab (Chionoecetes japonicus) shell by commercial proteases vol.45, pp.5, 2012, https://doi.org/10.4163/kjn.2012.45.5.429
  3. Changes in physicochemical characteristics of porcine blood under various conditions of enzyme hydrolysis vol.23, pp.3, 2016, https://doi.org/10.11002/kjfp.2016.23.3.413
  4. 효소 처리한 돈혈 활용 철분분말제제 특성 vol.23, pp.5, 2011, https://doi.org/10.11002/kjfp.2016.23.5.753
  5. Optimization and Pretreatment for Hot Water Extraction of Korean Deer (Cervus canadensis Erxleben) Velvet Antlers vol.30, pp.8, 2020, https://doi.org/10.4014/jmb.2004.04009