유기물자원화 (Journal of the Korea Organic Resources Recycling Association)

  • 제19권3호
  • /
  • Pages.63-72
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  • 2011
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  • 1225-6498(pISSN)
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  • 2508-3015(eISSN)
유기성자원학회 (Korea Organic Resources Recycling Association)
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Benzalkonium Chloride가 돈슬러리의 바이오가스 생성에 미치는 영향

Effect of Benzalkonium Chloride on Biogas Potential of Pig Slurry

  • 박혜림 (서울대학교 농생명공학부) ;
  • 최홍림 (서울대학교 농생명공학부) ;
  • Park, H. R. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Choi, H. L. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Suresh, Arumuganainar (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
  • 투고 : 2011.09.08
  • 심사 : 2011.09.28
  • 발행 : 2011.09.30
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초록

본 연구에서는 benzalkonium chloride 처리에 따라 바이오가스 생산이 억제되는 정도를 평가하였다. 바이오가스 생산 억제 수준은 10 ppm, 40 ppm, 80ppm의 benzalkonium chloride가 처리되었을 때 각각 10%, 30-40%, 70% 이상이었다. Benzalkonium chloride의 처리에 따라 저해되는 효소를 알아내기 위하여 효소 활성을 분석하였으며 산성/알칼리 포스파타아제, 프로테아제는 메탄 생산량과 음의 상관관계를 나타내었다. ${\alpha}$-글루코시다아제는 실험기간 동안 메탄 생성량과 상대적으로 낮은 음의 상관성을 보였으며(p<0.01, r=-426), 다른 효소와의 상관성도 상대적으로 낮았다. 메탄생성률(ml/day)은 benzalkonium chloride및 산성 포스파타아제와 유의한 상관성을 나타내었다. Benzalkonium chloride가 대장균에 미치는 영향을 원판확산법을 통하여 분석하였다. Benzalkonium chloride의 농도가 높을수록 세균증식 억제대가 확장되었으며, 이를 통하여 benzalkonium chloride가 초산생성균의 증식을 억제함으로써 혐기소화조에 영향을 미칠 수 있다는 것을 확인하였다.

Benzalkonium chloride is most widely used in S. Korea as a disinfectant in livestock husbandry. Inhibition of biogas potentials were investigated with three different doses of benzalkonium chloride in swine slurry. The system was operated at batch mode. The inhibition rates were 10%, 30~40% and >70% at the dose of 10ppm, 40ppm and 80ppm, respectively assuming it was zero percent in case of no dose. Enzymatic activities were analyzed to determine the enzymatic type which was inhibited by benzalkonium chloride. The acid phosphatase, alkaline phosphatase and protease were shown negatively correlated with biogas potential. Correlation of ${\alpha}$-glucosidase and biogas potentials was observed not high (p<0.01, r=-0.426) while benzalkonium chloride (r=-0.853, p<0.01) and acid phosphatase (p<0.01) with biogas potentials were significantly and negatively correlated. The effect of benzalkonium chloride on Escherichia coli were also evaluated by disc diffusion method. As increase of benzalkonium concentration, inhibition zone of anaerobic bacteria was extended. It revealed that benzalkonium chloride significantly deteriorated biogas potential through inhibition of acetogenic bacteria.

키워드

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