Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Simple and Disposable Chicken Cages for Experimental Eimeria Infections

  • Yoo, Jeong-Mi (College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University) ;
  • Kang, Sung-H. (College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University) ;
  • Jeong, Jip-Seol (College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Woo-H. (College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Suk (College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University) ;
  • Lillehoj, Hyun S. (Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U.S. Department of Agriculture) ;
  • Min, Won-Gi (College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University)
  • Received : 2011.06.10
  • Accepted : 2011.08.03
  • Published : 2011.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

During experimental Eimeria infections in chickens, facilities are often contaminated by fecal oocysts known to be highly resistant to both chemical and enzymatic treatments. Thus, studies using experimental Eimeria infections have been limited due to the difficulty of complete elimination of residual oocysts from both cages and facilities. To overcome this limitation, simple, inexpensive, and disposable cages were constructed from cardboard boxes and tested during experimental Eimeria maxima infections. The cages were used in animal rooms with only a 1.7% evidence of coccidia contamination between adjacent cages. No significant differences in fecal oocyst output and body weight gain were noted between animals housed in disposable cages and animals housed in wire control cages. This cage design is a useful means for preventing oocyst contamination during experimental conditions, suggesting that this disposable cage design could be used for other avian infectious disease studies.

Keywords

References

  1. Lee BH, Kim WH, Jeong J, Yoo J, Kwon YK, Jung BY, Kwon JH, Lillehoj HS, Min W. Prevalence and cross-immunity of Eimeria species on Korean chicken farms. J Vet Med Sci 2010; 72: 985-989. https://doi.org/10.1292/jvms.09-0517
  2. Sharman PA, Smith NC, Wallach MG, Katrib M. Chasing the golden egg: Vaccination against poultry coccidiosis. Parasite Immunol 2010; 32: 590-598.
  3. Min W, Dalloul RA, Lillehoj HS. Application of biotechnological tools for coccidian vaccine development. J Vet Sci 2004; 5: 279-288.
  4. Morris GM, Gasser RB. Biotechnological advances in the diagnosis of avian coccidiosis and the analysis of genetic variation in Eimeria. Biotechnol Adv 2006; 24: 590-603. https://doi.org/10.1016/j.biotechadv.2006.06.001
  5. Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE. Diseases of Poultry. 12th ed. Oxford, UK. Blackwell Publishing Co. 2008, p 1068-1085.
  6. Stotish RL, Wang CC, Meyenhofer M. Structure and composition of the oocyst wall of Eimeria tenella. J Parasitol 1978; 64: 1074-1081. https://doi.org/10.2307/3279729
  7. Tomley F. Techniques for isolation and characterization of apical organelles from Eimeria tenella sporozoites. Methods 1997; 13: 171-176. https://doi.org/10.1006/meth.1997.0509
  8. Kale HW 2nd, Jennings WL. A disposable cage for studies of pathogens in small wild birds. Am J Trop Med Hyg 1970; 19: 733-734.
  9. Cooper DM, Timms JR. The rearing and maintenance of breeding chickens in isolators. 2. Plastic tent isolators. Avian Pathol 1972; 1: 59-64. https://doi.org/10.1080/03079457208418052
  10. Dennett DP, Bagust TJ. Application of a flexible-film isolator for rearing specific pathogen-free chickens and investigating poultry pathogens. Avian Pathol 1979; 8: 289-300. https://doi.org/10.1080/03079457908418354
  11. Forder RE, Firth GA, Tivey DR, Howarth GS, Hughes RJ. A small-scale, low-cost isolation system for the incubation and rearing of low bacterial load chicks as a model to study microbial-intestinal interactions. Lab Anim 2008; 42: 185-192. https://doi.org/10.1258/la.2007.06021e
  12. Patton WH, Sapanski WB. An isolator for the collection of feces from chickens infected with coccidia. Lab Anim Sci 1974; 24: 924-928.
  13. Timms JR, Cooper DM, Millard BJ, Powell PC. An isolator for avian disease research. Lab Anim 1979; 13: 101-105. https://doi.org/10.1258/002367779780943378
  14. Chapman HD, Matsler PL, Muthavarapu VK, Chapman ME. Acquisition of immunity to Eimeria maxima in newly hatched chickens given 100 oocysts. Avian Dis 2005; 49: 426-429. https://doi.org/10.1637/7359-032805R1.1
  15. Dalloul RA, Lillehoj HS, Shellem TA, Doerr JA. Enhanced mucosal immunity against Eimeria acervulina in broilers fed a Lactobacillus-based probiotic. Poult Sci 2003; 82: 62-66.
  16. Lillehoj HS, Trout JM. Avian gut-associated lymphoid tissues and intestinal immune responses to Eimeria parasites. Clin Microbiol Rev 1996; 9: 349-360.

Cited by

  1. Identification and Comparative Expression Analysis of Interleukin 2/15 Receptor β Chain in Chickens Infected with E. tenella vol.7, pp.5, 2011, https://doi.org/10.1371/journal.pone.0037704