Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

Effects of Scutellariae Radix on Neuronal Apoptosis of Spinal Cord Contusion Injury in Rats

황금(黃芩)이 척수압박손상 흰쥐의 척수신경세포 자연사에 미치는 영향

  • Bahn, Hyo-Jung (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Jo, Jong-Jin (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Kim, Bum-Hoi (Dept. of Anatomy, College of Oriental Medicine, Dong-Eui University) ;
  • Park, Seong-Ha (Dept. of Internal Medicine, School of Korean Medicine, Pusan National University) ;
  • Shin, Jung-Won (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Kim, Seong-Joon (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Sohn, Nak-Won (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
  • 반효정 (경희대학교 동서의학대학원 한의과학전공) ;
  • 조종진 (경희대학교 동서의학대학원 한의과학전공) ;
  • 김범회 (동의대학교 한의과대학 해부학교실) ;
  • 박성하 (부산대학교 한의학전문대학원 한방내과학교실) ;
  • 신정원 (경희대학교 동서의학대학원 한의과학전공) ;
  • 김성준 (경희대학교 동서의학대학원 한의과학전공) ;
  • 손낙원 (경희대학교 동서의학대학원 한의과학전공)
  • Received : 2011.05.04
  • Accepted : 2011.09.23
  • Published : 2011.10.30
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Abstract

Objectives : This Study was performed to evaluate the effects of Scutellariae Radix(SR) water-extract on the tissue and neuronal apoptosis of the spinal cord injury(SCI). Methods: SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rats. SR was orally given once a day for 7 days after SCI. Neuronal apoptosis was examined with terminal deoxynucleotidyl transferase-mediated dUTPnick-end labeling(TUNEL) assay. Bax (Bcl-2-asociated X protein), Bcl-2(B-cell blastoma 2), c-Fos(FBJ osteosarcoma oncogene) expressions were examined using immuno-histochemistry. Individual TUNEL and immuno-labeled cells expressing Bax, Bcl-2 and c-Fos were counted on the same level in peri-damaged region and in ventral horn. Results: 1. SR significantly reduced number of TUNEL labeled apoptotic cells induced by the spinal cord contusion injury. 2. SR significantly reduced Bax positive cells expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. 3. SR strengthened Bcl-2 expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. 4. SR reduced c-Fos expression on the motor neuron in the ventral horn induced by the spinal cord contusion injury. Conclusions : These results suggest that SR plays an inhibitory role against neuronal apoptosis and has significant effects for locomotor disfunction induced by SCI.

Keywords

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