The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory effects of Chrysanthemum boreale flower

산국 꽃의 항염 활성 연구

  • You, Ki-Sun (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Bang, Chan-Sung (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Kyung-Jin (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Ham, In-Hye (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Ho-Young (Department of Herbology, College of Oriental Medicine, Kyung Hee University)
  • 유기선 (경희대학교 한의과대학 본초학교실) ;
  • 방찬성 (경희대학교 한의과대학 본초학교실) ;
  • 이경진 (경희대학교 한의과대학 본초학교실) ;
  • 함인혜 (경희대학교 한의과대학 본초학교실) ;
  • 최호영 (경희대학교 한의과대학 본초학교실)
  • Received : 2011.11.01
  • Accepted : 2011.12.16
  • Published : 2011.12.30
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Abstract

Objectives : Chrysanthemum boreale flower is widely distributed in Korea, Japan, China, and Eastern countries. C. boreale flower is also one of the herbs used for the treatment of various inflammatory disease in Korean Medicine. So, this research was designed to study anti-inflammatory effect of C. boreale flower and its mechanism. Methods : We investigated nitro oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production by ELISA. And expressions of inducible nitric oxide synthase (iNOS), Cyclooxygenase-2 (COX-2) and nuclear factor-${\kappa}B$ P50/65 (NF-${\kappa}B$ P50, NF-${\kappa}B$ P65) were measured in RAW 264.7 murine macrophage cells induced by LPS. Results : MeOH ex., EtOAc fr., $CHCl_3$ fr. and Water fr. of C. boreale flower showed anti-inflammatory effect through inhibition of NO and PGE expression respectively. Among them, EtOAc fr. and $CHCl_3$ fr. inhibited production of NO and $PGE_2$ through inhibition of iNOS and COX-2 expression. And MeOH ex., EtOAc fr. and $CHCl_3$ fr. inhibited translocation of NF-${\kappa}B$ P65, NF-${\kappa}B$ P50 by inhibiting phosphrylation of $I{\kappa}B$. Conclusions : MeOH ex. EtOAc fr, $CHCl_3$ fr., and Water fr. of the C. boreale flower have anti-inflammatory activity.

Keywords

References

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