대한본초학회지 (The Korea Journal of Herbology)

  • 제26권4호
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  • Pages.67-74
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  • 2011
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

허혈·재관류 유도 신경세포사멸에 대한 일당귀 물추출물의 신경보호효과 연구

Neuroprotective effects of Angelicae Acutilobae Radix water extract against ischemia·reperfusion-induced apoptosis in SK-N-SH neuronal cells

  • 오태우 (동국대학교 한의과대학 본초학교실) ;
  • 박기호 (동국대학교 한의과대학 본초학교실) ;
  • 이미영 (한국한의학연구원) ;
  • 최고야 (한국한의학연구원) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Oh, Tae-Woo (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Park, Ki-Ho (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Lee, Mi-Young (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Choi, Go-Ya (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
  • 투고 : 2011.11.08
  • 심사 : 2011.12.16
  • 발행 : 2011.12.30
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초록

Objectives : The purpose of the study is to determine the neuroprotective effects of the water extract of Angelicae Acutilobae Radix(AA) on ischemia reperfusion-induced apoptosis in SK-N-SH human brain neuronal cells. Methods: SK-N-SH cells were treated with different concentrations of AA water extract (0.1, 0.2, 0.5 and 1.0 mg/ml) for 2 hr and then stimulated with Dulbecco's phosphate-buffered saline containing CI-DPBS: 3mM sodium azide and 10 mM 2-deoxy-D-glucose for 45 min, reperfused with growth medium, and incubated for 24 h. Cell viability was determined by WST-1 assay, and ATP/ADP levels were measured by ADP/ATP ratio assay kit. The levels of caspase-3 protein were determined by Western blot and apoptotic body was observed by Hoechst 33258 staining. Results : AA extract significantly inhibited decreasing the cell viability in ischemia-induced SK-N-SH cells. AA also increased the ratio of ADP/ATP in ischemia-induced neuronal cells and decreased the expression levels of apoptotic protein, caspase-3 and apoptotic DNA damage. Conclusions : Our results suggest that AA extract has a neuroprotective property via suppressing the apoptosis and increasing the energy levels in neuronal cells, suggesting that AA extract may has a therapeutic potential in the treatment of ischemic brain injury.

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