The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective effect of Socheongryong-Tang on hydrogen peroxide-induced hepatotoxicity

소청룡탕(小靑龍湯)의 과산화수소로 유도된 간세포 독성에 대한 보호효과

  • Lee, Ji-Seon (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Oh, Su-Young (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Seo, Sang-Hee (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Tae-Soo (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
  • Received : 2011.11.04
  • Accepted : 2011.12.16
  • Published : 2011.12.30
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Abstract

Objectives : Socheongryong-Tang (小靑龍湯, SCRT) has been widely used to treat respiratory disease. In this study, we investigated the protective effects of SCRT on hydrogen peroxide-induced hepatotoxicity. Methods : In the mouse primary liver cells, SCRT was pretreated for 1 h, and 1 mM $H_2O_2$ was treated to mouse primary liver cells. Cell viability was analyzed by using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Also, the activity of AST, ALT and LDH were measured for the evaluation the protective effect of SCRT on $H_2O_2$-induced hepatotoxicity. Intracellular ROS level was analyzed by FACS. Results : SCRT pretreatment decreased $H_2O_2$-induced hepatotoxicity and intracellular ROS production. Pretreatment of SCRT significantly reduced the cytotoxic effect induced by $H_2O_2$, associated with reducing DNA fragmentation and AST, ALT, LDH activities. Conclusions : These results suggest that SCRT has protective effect against $H_2O_2$-induced hepatotoxicity.

Keywords

References

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