Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Heat and pH Treatments on α-Amylase Inhibitory Activity of Ecklonia cava Ethanol Extract

감태(Ecklonia cava) 에탄올 추출물의 α-Amylase 저해활성에 미치는 열 및 pH의 영향

  • Kim, Dong-Hyun (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Jung, Ji-Yeon (Food Research Center, CJ Research Institute) ;
  • Kim, Koth-Bong-Woo-Ri (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Lee, Chung-Jo (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Kwak, Ji-Hee (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Min-Ji (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • SunWoo, Chan (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Hyun-Jee (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Jung, Seul-A (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Tae-Wan (Department of Food Science and Technology, Andong National University) ;
  • Cho, Young-Je (School of Food Science of Biotechnology, Kyungpook National University) ;
  • Ahn, Dong-Hyun (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
  • 김동현 (부경대학교 식품공학과/식품연구소) ;
  • 정지연 (CJ 식품연구소) ;
  • 김꽃봉우리 (부경대학교 식품공학과/식품연구소) ;
  • 이청조 (부경대학교 식품공학과/식품연구소) ;
  • 곽지희 (부경대학교 식품공학과/식품연구소) ;
  • 김민지 (부경대학교 식품공학과/식품연구소) ;
  • 선우찬 (부경대학교 식품공학과/식품연구소) ;
  • 김현지 (부경대학교 식품공학과/식품연구소) ;
  • 정슬아 (부경대학교 식품공학과/식품연구소) ;
  • 김태완 (안동대학교 식품생명공학과) ;
  • 조영제 (경북대학교 식품공학부) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2011.10.07
  • Accepted : 2011.12.08
  • Published : 2011.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study examined the inhibitory activity of Ecklonia cava (EC) against ${\alpha}$-amylase to evaluate the availability of EC extract as a functional food agent. To verify the inhibitory activity of EC against porcine pancreatic ${\alpha}$-amylase, potato starch was used as a substrate. This analysis revealed that EC ethanol extract exhibited high ${\alpha}$-amylase inhibitory activity. For potential application within the food industry, the stability of the activity of EC ethanol extract under various heat and pH conditions was examined. The ${\alpha}$-amylase inhibitory activity of EC ethanol extract was not affected by the heat and pH treatment conditions used in this study. These results suggest that EC has the potential for development as a functional food agent.

Keywords

References

  1. Ali H, Houghon PJ and Soumyanath A. 2006. $\alpha$-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 107, 449-455. https://doi.org/10.1016/j.jep.2006.04.004
  2. Bhandari MR, Jong-Anurakkun N and Kawabata J. 2008. $\alpha$-Glucosidase and $\alpha$-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliate Haw.). Food Chem 106, 247-252. https://doi.org/10.1016/j.foodchem.2007.05.077
  3. Birari RB and Bhutani KK. 2007. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov Today 12, 879-889. https://doi.org/10.1016/j.drudis.2007.07.024
  4. Burtin P. 2003. Nutritional value of seaweeds. EJEAF Che 2, 498-503.
  5. Chrzaszcz T and Janicki J. 1933. "Sisto-amylase", a natural inhibitor of amylase. Biochem Zeitschrift 260, 364-368.
  6. de Melo EB, Gomes AS and Carvalho I. 2006. $\alpha$- and $\beta$-Glucosidase inhibitors : chemical structure and biological activity. Tetragedron 62, 10277-10302. https://doi.org/10.1016/j.tet.2006.08.055
  7. Deavile ER, Green RJ, Muller-Harvey I, Willoughby I and Frazier RA. 2007. Hydrolyzable tannin structures influence relative globular and random coil protein binding strengths. J Agr Food Chem 55, 4554-4561. https://doi.org/10.1021/jf063770o
  8. Franz MJ, Bantle JP, Beebe CA, Brunzeu JD and Chasson JL. 2002. Evidence-based nutrition principles and recommendations for the treatment and prevention of diabetes and related complications. Diabetes Care 25, 148-198. https://doi.org/10.2337/diacare.25.1.148
  9. Gupta S and Abu-ghannam N. 2011. Bioactive potential and possible health effects of edible brown seaweeds. Trends Food Sci Tech 22, 315-326. https://doi.org/10.1016/j.tifs.2011.03.011
  10. Hanefeld M. 1998. The role of acarbose in the treatment of non-insulin-dependent diabetes mellitus. J Diabetes Complicat 12, 228-237. https://doi.org/10.1016/S1056-8727(97)00123-2
  11. Itsuo S, Makoto O, Tomio Y, Atsushi O, Choitsu S, Hosai Y, Mitsuo M and Shigeaki B. 1981. Effect of $\beta$-glucosidase inhibitor on human pancreatic and salivary $\beta$-amylase. Clin Chim Acta 117, 145-152. https://doi.org/10.1016/0009-8981(81)90035-8
  12. Jung JY. 2011. Lipase inhibitory activity of Ecklonia cava extracts. MS Thesis, Pukyong National University, Busan, Korea.
  13. Jung SH. 1997. Development and application of enzyme inhibitor. In: Regulation of enzyme activity. Oh SH, ed. Doseo Press, Seoul, Korea. 1-2.
  14. Kageyama S, Nakamichi N, Sekino H and Nakano S. 1997. Comparison of the effect of acarbose and voglibose in healthy subjects. Clin Ther 19, 720-729. https://doi.org/10.1016/S0149-2918(97)80096-3
  15. Kang KA, Lee KH, Chae SW, Zhang R, Jung MS, Lee YK, Kim Y, Kim HS, Joo HG, Park JW, Ham MY, Lee NH and Hyun JW. 2005. Eckol isolated from Ecklonia cava attenuates oxidative stress induced cell damage in lung fibroblas cells. FEBS Lett 579, 6295-6304. https://doi.org/10.1016/j.febslet.2005.10.008
  16. Kim AR, Song EJ, Kim MJ, Lee SY, Kim KBWR, Kim JH, Kim SJ, Hong YK, Park JG, Kim JH, Lee JW, Byun MW and Ahn DH. 2008a. Effect of gamma irradiation on antioxidant properties and physical characteristics of Sargassum siliquastrum water extract. J Korean Soc Food Sci Nutr 37, 357-361. https://doi.org/10.3746/jkfn.2008.37.3.357
  17. Kim JH, Kim MU and Cho YJ. 2007. Isolation and identification of inhibitory compound from Crataegi fructus on $\beta$-amylase and $\beta$-glucosidase. J Korean Soc Appl Biol Chem 50, 204-209.
  18. Kim KH, Roh SG, Li CR, Jin CF, Kim A and Choi WC. 2008b. Anti-diabetic effects of banaba leaf extracts (Lagerstroemia speciosa Pers.) through solvents. J Life Sci 18, 1305-1311. https://doi.org/10.5352/JLS.2008.18.9.1305
  19. Kim MJ, Choi JS, Song EJ, Lee SY, Kim KBWR, Lee SJ, Kim SJ, Yoon SY, Jeon YJ and Ahn DH. 2009. Effects of heat and pH treatments on antioxidant properties of Ishige okamurai extract. Korean J Food Sci Technol 41, 50-56.
  20. Krentz AJ, Clough G and Byrne CD. 2007. Interactions between microvascular and macrovascular disease in diabetes: pathophysiology and therapeutic implications. Diabetes Obes Metab 9, 781-791. https://doi.org/10.1111/j.1463-1326.2007.00670.x
  21. Lee BB, Park SR, Han CS, Han DY, Park EJ, Park HR and Lee SC. 2008. Antioxidant activity and inhibition activity against $\alpha$-amylase and $\alpha$-glucosidase of Viola mandshurica extracts. J Korean Soc Food Sci Nutr 37, 405-409. https://doi.org/10.3746/jkfn.2008.37.4.405
  22. Lee CJ, Song EJ, Kim KBWR, Jung JY, Kwak JH, Choi MK, Kim MJ, Kim DH, Sunwoo C, Park JG, Kim JH, Choi JI, Lee JW, Byun MW and Ahn DH. 2011. Effect of gamma irradiation on immune activity and physicochemical properties of Myagropsis myagroides water extract. Kor J Fish Aquat Sci 44, 50-57.
  23. Lee SH, Han JS, Heo SJ, Hwang JY and Jeon YJ. 2010a. Protective effects of dieckol isolated from Ecklonia cava against high glucose-induced oxidative stress in human umbilical vein endothelial cells. Toxicol In Vitro 24, 375-381. https://doi.org/10.1016/j.tiv.2009.11.002
  24. Lee SH, Li Y, Karadeniz F, Kim MM and Kim SK. 2009a. $\alpha$-Glucosidase and $\alpha$-amylase inhibitory activities of phloroglucinal derivatives from edible marine brown alga, Ecklonia cava. J Sci Food Agric 89, 1552-1558. https://doi.org/10.1002/jsfa.3623
  25. Lee SH, Park MH, Heo SJ, Kang SM, Ko SC, Han JS and Jeon YJ. 2010b. Dieckol isolated from Ecklonia cava inhibits $\alpha$-glucosidase and $\alpha$-amylase in vitro and alleviates postprandial hyperglycemia in streptozotocin-induced diabetic mice. Food Chem Toxicol 48, 2633-2637. https://doi.org/10.1016/j.fct.2010.06.032
  26. Lee SJ, Song EJ, Kim KBWR, Lee CJ, Jung JY, Kwak JH, Choi MK, Kim MJ, Kim TW and Ahn DH. 2010c. Inhibitory effects of Sargassum thunbergii ethanol extract against $\alpha$-amylase. Kor J Fish Aqua Sci 43, 648-653.
  27. Lee SJ, Song EJ, Lee SY, Kim KBWR, Kim SJ, Yoon SY, Lee CJ, and Ahn DH. 2009b. Antioxidant activity of leaf, stem and root extracts from Orostachys japonicus and their heat and pH stabilities. J Korean Soc Food Sci Nutr 38, 1571-1679. https://doi.org/10.3746/jkfn.2009.38.11.1571
  28. Lee SY, Song EJ, Kim KBWR, Yoon SY, Kim SJ, Lee SJ, Hong YK, Lim SM and Ahn DH. 2009c. Antimicrobial activity of ethanol extract from Sargassum thunbergii. J Korean Soc Food Sci Nutr 38, 502-508. https://doi.org/10.3746/jkfn.2009.38.4.502
  29. Liener I. 1979. Significance for humans of biologically active factors in soybeans and other food legumes. J Am Oil Chem Soc 56, 121-129. https://doi.org/10.1007/BF02671433
  30. Lim CS, Li CY, Kim YM, Lee WY and Rhee HI. 2005. The inhibitory effect of Cornus walteri extract against $\alpha$-amylase. J Korean Soc Appl Biol Chem 48, 103-108.
  31. McCue PP and Shetty K. 2004. Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asia Pac J Clin Nutr 13, 101-106.
  32. Mulimani VH and Rudrappa G. 1994. Effect of heat treatment and germination on alpha amylase inhibitor activity in chick peas (Cicer arietinum L.). Plant Food Hum Nutr 46, 133-137. https://doi.org/10.1007/BF01088765
  33. Park JH, Baek MR, Lee BH, Yon GH, Ryu SY, Kim YS, Park SU and Hong KS. 2009. $\alpha$-Glucosidase and $\alpha$-amylase inhibitory activity of compounds from roots extract of Pueraria thunbergiana. Korean J Medicinal Crop Sci 17, 357-362.
  34. Park NB, Song EJ, Kim KBWR, Lee CJ, Jung JY, Kwak JH, Choi MK, Kim MJ, Nam KW and Ahn DH. 2010. Antimicrobial activity of Myagropsis yendoi extract. Kor J Fish Aquat Sci 43, 642-647.
  35. Saini AK, Kumar HSA and Sharma SS. 2007. Preventive and curative effect of edaravone on nerve functions and oxidative stress in experimental diabetic neuropathy. Eur J Pharmacol 568, 164-172. https://doi.org/10.1016/j.ejphar.2007.04.016
  36. Shin HC, Hwang HJ, Kang KJ and Lee BH. 2006. An antioxidative and antiinflammatory agent for potential treatment of osteoarthritis from Ecklonia cava. Arch Pharm Res 29, 165-171. https://doi.org/10.1007/BF02974279
  37. Turner R, Hattersley A and Cook J. 1992. Type II. diabetes: search for primary defects. Ann Med 24, 511-516. https://doi.org/10.3109/07853899209167004
  38. Weselake RJ, Macgregor AW, Hill RD and Duckworth HW. 1983. Purification and characteristics of an endogenous $\alpha$-amylase inhibitor from barley kernels. Plant Physiol 73, 1008-1012. https://doi.org/10.1104/pp.73.4.1008
  39. Yoon NY, Eom TK, Kim MM and Kim SK. 2009. Inhibitory effect of phlorotannins isolated from Ecklonia cava on mushroom tyrosinase activity and melanin formation in mouse B16F10 melanoma cells. J Agric Food Chem 57, 4124-4129. https://doi.org/10.1021/jf900006f
  40. Yoon SY, Lee SY, Kim KBWR, Song EJ, Lee SJ, Lee CJ, Park NB, Jung JY, Kwak JH, Nam KW and Ahn DH. 2010. Antimicrobial activity of the Sargassum fulvellum ethanol extract and the effect of temperature and pH on their activity. Korean J Food Sci Technol 42, 155-159.
  41. Zhuang C, Itoh H, Mizuno T and Ito H. 1995. Antitumor active fucoidan from the brown seaweed, umitoranoo (Sargaum thunbergii ). Biosci Biotech Bioch 59, 563-567. https://doi.org/10.1271/bbb.59.563
  42. Zimmet P, Alberti K and Shaw J. 2001. Global and societal implications of the diabetes epidemic. Nature 414, 782-787. https://doi.org/10.1038/414782a