Effects of Root of Scutellariae Radix against Inflammatory Response in the Spinal Cord Contusion Injury in Rats

척수압박손상 흰쥐의 척수조직 염증반응에 황금(黃芩)이 미치는 영향

  • Yang, Kee-Young (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Choi, Won-Ik (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Shin, Jung-Won (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Park, Seong-Ha (Dept. of Internal Medicine, School of Korean Medicine, Pusan National University) ;
  • Kim, Seong-Joon (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Lee, Jong-Soo (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Sohn, Nak-Won (Dept. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
  • 양기영 (경희대학교 동서의학대학원 한의과학전공) ;
  • 최원익 (경희대학교 동서의학대학원 한의과학전공) ;
  • 신정원 (경희대학교 동서의학대학원 한의과학전공) ;
  • 박성하 (부산대학교 한의학전문대학원 한방내과학교실) ;
  • 김성준 (경희대학교 동서의학대학원 한의과학전공) ;
  • 이종수 (경희대학교 한의과대학 한방재활의학교실) ;
  • 손낙원 (경희대학교 동서의학대학원 한의과학전공)
  • Received : 2011.05.03
  • Accepted : 2011.07.04
  • Published : 2011.07.30

Abstract

Objectives : This study was performed to evaluate the effects of root of Scutellariae Radix(SR) water extract against inflammatory response in the spinal cord injury(SCI). Methods : SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rat. SR was orally given once a day for 7days after SCI. Myeloperoxidase(MPO) positive neutrophils infiltration was examined. Inducible nitric oxide synthase(iNOS) and tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) expressions were observed with immunohistochemistry. Glial fibrillary acidic protein(GFAP) positive astrocytes were examined using immuno-fluorescence. Results : 1. SR reduced MPO-positive neutrophils infiltration in peri-damage regions of the contusive SCI-induced rats. 2. SR reduced iNOS positive cells in the white matter of the contusive SCI-induced rats. 3. SR reduced TNF-${\alpha}$ positive cells in the gray and white matter of the contusive SCI-induced rats. 4. SR reduced cell number and size of astrocytes in peri-damage regions of the contusive SCI-induced rats. Conclusions : These results suggest that SR plays an inhibitory role against inflammatory response in the SCI.

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