Journal of Animal Science and Technology

  • 제53권1호
  • /
  • Pages.7-13
  • /
  • 2011
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  • 2672-0191(pISSN)
  • /
  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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한우 Exostosin-1 유전자의 SNP 탐색 및 경제형질 관련성 분석

Association Study Between the Polymorphisms of Exostosin-1 Gene and Economic Traits in Hanwoo

  • 김범수 (농촌진흥청 국립축산과학원) ;
  • 김남국 (농촌진흥청 국립축산과학원) ;
  • 이승환 (농촌진흥청 국립축산과학원) ;
  • 조용민 (농촌진흥청 기획조정관실) ;
  • 허강녕 (농촌진흥청 국립축산과학원) ;
  • 박응우 (농촌진흥청 연구정책국) ;
  • 양부근 (강원대학교 동물생명과학대학 동물자원과학) ;
  • 윤두학 (농촌진흥청 국립축산과학원)
  • Kim, Bum-Soo (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R. D. A.) ;
  • Kim, Nam-Kuk (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R. D. A.) ;
  • Lee, Seung-Hwan (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R. D. A.) ;
  • Cho, Yong-Min (Green Growth & Future Strategy Team, R. D. A.) ;
  • Heo, Kang-Nyeong (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R. D. A.) ;
  • Park, Eung-Woo (Coopreration & Governance Unit Research Policy Planning Division Research Policy Bureau, R. D. A.) ;
  • Yang, Boo-Keun (Department of Animal Resources Science, Kangwon National University) ;
  • Yoon, Du-Hak (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, R. D. A.)
  • 투고 : 2010.08.11
  • 심사 : 2010.12.02
  • 발행 : 2011.02.28
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초록

본 연구는 한우의 경제형질 관련 유전적 표지인자(DNA marker) 개발을 목적으로 EXT1 유전자의 다형성과 발현양상을 관찰하여 경제형질과의 관련성을 확인하고자 수행하였다. PCRdirect sequencing을 통하여 4개(T272196A, C272359T, G290964A 및 A302092G)의 SNPs를 탐색 하였으며, 탐색된 SNPs를 통하여 PCR-RFLP 기법으로 유전자형을 결정한 후 경제형질과 관련성을 분석하였다. 그 결과 T272196A 좌위에서 근내지방 육종가(p=0.014), G290964A 좌위에서 등지방두께 육종가 추정치(p=0.001), A302092G 좌위에서는 등지방두께 육종가(p < 0.001) 및 등심단면적 육종가(p=0.020)에서 각각 유의적인 연관성이 인정되었다. 따라서, 본 연구를 통해 확인된 SNP를 이용하여 한우의 선발에 활용 가능할 것으로 사료된다.

The aim of this study was to identify the polymorphism on exostosin-1 (EXT1) gene and to associate with economic traits in Hanwoo (Korean cattle). We sequenced for detection of single nucleotide polymorphism (SNP) with 24 unrelated individuals and identified four SNPs (T272196A, C272359T, G290964A and A302092G). Relationship between the genotypes of 583 Hanwoo individuals by PCR-RFLP and economic traits were analyzed by general linear model. In EXT1 gene, there were four SNPs associated with economic traits such as eye muscle area breeding value, marbling score breeding value, backfat and thickness breeding value (p<0.05 to p<0.01). In conclusion, this study indicates an important role of EXT1 gene in determining the meat quality or economic characteristics in Hanwoo.

키워드

참고문헌

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피인용 문헌

  1. Association between the Polymorphism of the Fatty acid binding protein 5 (FABP5) Gene within the BTA 14 QTL Region and Carcass/Meat Quality Traits in Hanwoo vol.53, pp.4, 2011, https://doi.org/10.5187/JAST.2011.53.4.311
  2. Identification of a SNP in Cattle HGD Gene with its Effect on Economic Trait in Hanwoo vol.24, pp.11, 2014, https://doi.org/10.5352/JLS.2014.24.11.1168
  3. Application of DNA marker related with marbling score in Hanwoo cow vol.27, pp.3, 2016, https://doi.org/10.7465/jkdi.2016.27.3.733
  4. Validation of DNA Markers for Carcass Traits with Commercial Hanwoo Population vol.48, pp.2, 2014, https://doi.org/10.14397/jals.2014.48.2.133
  5. Association Study Between the SNP of DECR1 Gene and Economic Traits in Hanwoo vol.48, pp.3, 2014, https://doi.org/10.14397/jals.2014.48.3.139