DOI QR코드

DOI QR Code

Effects of Fermented Turmeric Extracts on the Obesity in Rats Fed a High-Fat Diet

강황 발효액이 고지방 섭취 흰쥐의 비만에 미치는 효과

  • Yang, Cheul-Young (College of Health Industry, Eulji University) ;
  • Cho, Mi-Jin (Department of Food Science and Biotechnology of Animal Resources, College of Animal Bioscience & Technology, Konkuk University) ;
  • Lee, Chi-Ho (Department of Food Science and Biotechnology of Animal Resources, College of Animal Bioscience & Technology, Konkuk University)
  • 양철영 (을지대학교 보건산업대학) ;
  • 조미진 (건국대학교 동물생명과학대학) ;
  • 이치호 (건국대학교 동물생명과학대학)
  • Received : 2010.08.27
  • Accepted : 2010.10.15
  • Published : 2011.02.28

Abstract

This study was designed to investigate the effects of fermented turmeric extract on body weight, abdominal fat mass and biochemical markers related to obesity in rats fed high fat diet. Turmeric and brown sugar was equally mixed and fermented for one year. Wistar rats (weighing about 125 g (15 rats)) were divided into three groups: high fat diet (20.9%) and brown sugar 7.2% (HFD), turmeric powder in replace of corn starch 12.8%, brown sugar 7.2% (TP), fermented turmeric powder 20% (FTP) for four weeks. The final body weight was about 425g. The retroperitoneal fat weights in TP group showed the tendency to decrease regardless of feed intake in the rats. Serum levels of total cholesterol and LDL-cholesterol in the FTP group showed the lowering tendency than those of the HFD group (p<0.05). Serum levels of leptin, adiponectin and aspartate aminotransferase (AST) in the FTP group was significantly (p<0.05) lower than that of the HFD group, and serum level of tumor necrosis factor-$\alpha$ (TNF-$\alpha$) in all the groups were similar. These results suggest that fermented turmeric extract might be effective to prevent obesity in rats fed high fat diet.

본 실험에서는 발효 강황 발효액의 급여가 내장지방과 혈청지질에 미치는 효과를 살펴보고자 수행하였다. 강황과 흑설탕을 동일비율로 혼합하여 12개월간 숙성 발효하였다. 발효액은 HPLC를 이용해 발효 후 유리당의 함량을 분석하였다. 동물실험은 총 3군으로 일반 대조군에는 고지방사료와 흑설탕 7.2% (HFD), 처리군에는 고지방 사료에 흑설탕 7.2%와 강황 12.8% (TP), 고지방 사료에 분말화한 발효 강황 20% (FTP)을 첨가한 배합사료를 4주간 급여 하였다. 혈청지질에서 총 콜레스테롤과 LDL-콜레스테롤은 HFD군에 비하여 모두 감소하는 경향이었다. 혈청 중 아디포넥틴과 렙틴은 HFD군에 비해서 FTP군이 유의적으로 낮았으며(P<0.05), TNF-$\alpha$은 HFD군에 비해서 FTP군이 감소하였다. 또한 간 손상 지표인 혈청의 AST는 HFD군에 비해서 FTP군이 감소하였다. 이를 통해 발효 강황 발효액은 발효 전보다 발효 후에 고지방식이를 투여한 흰쥐에 있어서 비만을 억제할 수 있는 가능성이 있는 것으로 사료되었다.

Keywords

References

  1. Aggarwal, B. B., Sundaram, C., Malani, M. and Ichikawa, H. 2007. Curcumin: The Indian solid gold. In the molecular targets and therapeutic uses of Curcumin in health and disease (ed by B. B. Aggarwal, Y-‐J. Surh, S. Shishodia). Springer Publishing Company. NewYork. pp. 1-76.
  2. Amon, H. P. T. and Wahl, M. A. 1991. Pharmacology of Curcuma longa. Planta Med. 57:1-7. https://doi.org/10.1055/s-2006-960004
  3. AOAC. 2005. Official methods analysis 18th ed. Association of official analytical chemists Washington D.C. USA. pp. 125-132.
  4. Asai, A. and Miyazawa, T. 2001. Dietary curcuminoids prevent highfat diet-induced lipid accumulation in rat liver and epididymal adipose tissue. J. Nutr. 131:2932-2935. https://doi.org/10.1093/jn/131.11.2932
  5. Berg, A. H. and Scherer, P. E. 2005. Adipose tissue, inflammation, and cardiovascular disease. Circ. Res. 96:939-949. https://doi.org/10.1161/01.RES.0000163635.62927.34
  6. Centers for Disease Control and Prevention (CDC). 2008. Cancer prevention and control.
  7. Chi, H. J. and Kim, H. S. 1983. Curcumin content of cultivated Tumeric in Korea. Korean J. Pharmacol. 14:67-69.
  8. Choi, S. W., Yang, J. S., Lee, H. S., Kim, D. S., Bae, D. H. and Yu, J. H. 2003. Characterization of squalene synthase inhibitor isolated from Curcuma longa. Korean J. Food Sci. Technol. 35:297-301.
  9. Despress, J. P. and Lemieux, I. 2006. Abdominal obesity and metabolic syndrome. Nature 444:881-887. https://doi.org/10.1038/nature05488
  10. Dirlewanger, M., Di Vetta, V., Giusti, V., Schneiter, P., Jequier, E. and Tappy, L. 1999. Effect of moderate physical activity on plasma leptin concentration in humans. Eur. J. Appl. Physiol. 79:331-335. https://doi.org/10.1007/s004210050516
  11. Ejaz, A., Wu, D., Kwan, P. and Meydani, M. 2009. Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice. J. Nutr. 139:919-925. https://doi.org/10.3945/jn.108.100966
  12. Eriko, K., Tetsuya, I., Koki, F. and Yoshihiro, K. 2006. A mixture of the Salacia reticulata (Kotala himbutu) aqueous extract and cyclodextrin reduced the accumulation of visceral fat mass in mice and rats with high-fat diet-induced obesity. J. Nutr. 136: 433-439. https://doi.org/10.1093/jn/136.2.433
  13. Ezzati, M., Martin, H., Skjold, S., Vander Hoorn, S. and Murray, C. J. 2006. Trends in national and state-level obesity in the USA after correction for self-report bias: analysis of health surveys. J. R. Soc. Med. 99:250-257. https://doi.org/10.1258/jrsm.99.5.250
  14. Folch, J., Lees, M. and Sloane-Stanley G. H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509. Gupta, B. and Ghash, B. 1999. Curcuma longa inhibits $TNF-{\alpha}$ induced expression of adhesion molecules human umbilical vein endothelial cells. Int. J. Immunopharmacol. 21:745-757. https://doi.org/10.1016/S0192-0561(99)00050-8
  15. Hotamisligik, G. S. 2006. Inflammation and metabolic disorder. Nature 444:860-867. https://doi.org/10.1038/nature05485
  16. Kang, J. K., Kang, H. J., Seo, J. H., Kim, S. O., Choi, J. H. and Cho, D. Y., 2009. Effect of fermented turmeric (Curcuma longa) by Bacillus natto supplementation on liver function and serum lipid parameters in mice. J. Korean Soc. Food Sci. Nutr. 38:430-435. https://doi.org/10.3746/jkfn.2009.38.4.430
  17. Kang, W. S., Kim, J. H., Park, E. J. and Yoon, K. R. 1998. Antioxidative property of turmeric (Curcuma rhizoma) ethanol extract. Korean J. Food Sci. Technol. 30:266-271.
  18. Kim, C. R. 2006. Enhancement of liver function by curcuma extract on acute hepatotoxicity in rat. Korean J. Food Sci. Ani. Resour. 26:386-393.
  19. Kim, M. O., Oh, S. C. and Kim, S. S. 2008a. Method of manufacturing Curcuma domestica fermented extract. Korean Patent. 842, 022.
  20. Kim, T. H., Son, Y. K., Hwang, K. H. and Kim, M. H. 2008b. Effects of Angelica keiskei Koidzumi and turmeric extract supplementation on serum lipid parameters in hypercholesterolemic diet or P-407-induced hyperlipidemic rats. J. Korean Soc. Food Sci. Nutr. 37:708-713. https://doi.org/10.3746/jkfn.2008.37.6.708
  21. Lee, S. G., Cho, K. H., Lee, K. S. and Koo, B. H. 1990. Effexs of cefotaxime on the hepatic and renal function of Curcuma rhizoma on rabbits. Kyung Hee Univ. Med. Cont. 6:188-210.
  22. Lee, J. and Jun, W. 2009. Methanolic extract of turmeric (Curcuma longa L.) enhanced the lipolysis by up-regulation of lipase mRNA expression in differentiated 3T3-L1 adipocytes. Food Sci. Biotechnol. 18:1500-1504.
  23. Liu, C. H., Huang, M. T. and Huang, P. C. 1995. Sources of triglycerol accumulation in liver of rats fed a cholesterol supplemented diets. Lipids 30:527-531. https://doi.org/10.1007/BF02537027
  24. Mazumber, A., Raghavan, K., Weinstein, J. and Kohn, K. W. 1995. Pommiery inhibition of human immunodifficiency virus TYPE-1 integrase by curcumin. Biochem. Pharmacol. 49:1165-1170. https://doi.org/10.1016/0006-2952(95)98514-A
  25. Park, E. J., Jeon, C. H., Ko, G., Kim, J. and Sohn, D. H. 2000. Protective effect of curcumin in rat liver injury induced by carbon tetrachloride. J. Pharm. Pharmacol. 52:437-40. https://doi.org/10.1211/0022357001774048
  26. Ryu, G. Y., No, K. H., Ryu, S. R. and Yang, H. S. 2005a. Study of separation and analysis method an effective component from ulgeum (Curcuma longa) and a contained Curcumin as product of national and partially region cultures. Applied Chemistry. 9:57-60.
  27. Ryu, S. R., Han, K. J. and Jang, H. D. 2005b. Separation and purification of effectiveness components from ulgeum (Curcuma longa) & the test study of anticancer effects that use it. Applied chemistry. 9:69-72.
  28. Scherer, P. E., Williams, S., Fogliano, M., Baldini, G. and Lodish, H. F. 1995. A novel serum protein similar to C1q, produced exclusively in adipocytes. J. Biol. Chem. 270:26746-26749. https://doi.org/10.1074/jbc.270.45.26746
  29. Weisberg, S. P., Leibel, R. and Tortoriello, D. V. 2008. Dietary curcumin significantly improves obesity-associated inflammation and diabetes in mouse models of diabetic. Endocrinology. 149:3549-3558. https://doi.org/10.1210/en.2008-0262
  30. Wiecek, A., Kokot, F., Chudek, J. and Adamczak, M. 2002. The adipose tissue-a novel endocrine organ of interest to the nephrologist. Nephrol. Dial. Transplant. 17:191-195. https://doi.org/10.1093/ndt/17.2.191
  31. Wilson, A. M., Ryan, M. C. and Boyle, A. J. 2006. The novel role of C-reactive protein in cardiovascular disease: Risk marker or pathogen. Int. J. Cardiol. 106:291-297. https://doi.org/10.1016/j.ijcard.2005.01.068

Cited by

  1. Anti-obesity Effects of Capsule-filled Cheonggukjang Added with Arrowroot (Pueraria thunbergiana) Extracts in ob/ob Mice vol.41, pp.6, 2012, https://doi.org/10.3746/jkfn.2012.41.6.782
  2. Antioxidant and ACE Inhibiting Activities of Sugared-Buchu (Allium ampeloprasum L. var. porum J. Gay) Fermented with Lactic Acid Bacteria vol.24, pp.6, 2014, https://doi.org/10.5352/JLS.2014.24.6.671
  3. Investigation of Factors on the Sensory Characteristics of Milk Bread with Tumeric Powder (Curcuma longa L.) Using Fractional Factorial Design Method vol.43, pp.4, 2014, https://doi.org/10.3746/jkfn.2014.43.4.592
  4. Microbial Population of Foodborne Pathogens during Fermentation of Mulberry Wort vol.31, pp.6, 2016, https://doi.org/10.13103/JFHS.2016.31.6.458
  5. Phenolics and eudesmanolide from aged common sage exudate with sugar vol.26, pp.6, 2017, https://doi.org/10.1007/s10068-017-0234-8