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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Identification of EphA7 BAC clone containing a long-range dorsal midline-specific enhancer

  • Kim, Yu-Jin (Department of Biological Science, Sookmyung Women's University) ;
  • Park, Soo-Chul (Department of Biological Science, Sookmyung Women's University)
  • Received : 2010.12.18
  • Accepted : 2010.12.22
  • Published : 2011.02.28
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Abstract

Previous studies suggest that EphA7 plays a critical role in neural tube closure or cortical progenitor apoptosis. In this report, enhancer trap assay was used to modify various EphA7 BAC clones and screen a large genomic region spanning 570 kb downstream of the EphA7 gene. We found that the dorsal midline-specific EphA7 enhancer resides on the 457D20 EphA7 BAC clone and is localized to a 35 kb genomic region in between +345.7 kb to +379.8 kb downstream of the EphA7 transcription start site. Identification of the EphA7 BAC clone containing a long-range dorsal midline enhancer may constitute a useful tool for investigating the biological functions of EphA7 in vivo.

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References

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