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Antioxidant and Antihypertensive Activities of Styela plicata according to Harvesting Time and Size

채취시기와 크기에 따른 주름 미더덕의 항산화 및 항고혈압 활성

  • Park, Ji-Won (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • You, Dong-Hyun (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Bae, Myung-Suk (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Jung-Mi (Dept. of Food and Nutrition, Kyungnam University) ;
  • Lee, Jong-Hwa (Dept. of Food Science and Biotechnology, Andong National University) ;
  • Kim, Suk-Ju (Dept. of Food Nutrition and Cookery, Woosong Information College) ;
  • Jeon, Yuo-Jin (Faculty of Marine Biomedical Sciences, Jeju National University) ;
  • Park, Eun-Ju (Dept. of Food and Nutrition, Kyungnam University) ;
  • Lee, Seung-Cheol (Dept. of Food Science and Biotechnology, Kyungnam University)
  • 박지원 (경남대학교 식품생명학과) ;
  • 유동현 (경남대학교 식품생명학과) ;
  • 배명숙 (경남대학교 식품생명학과) ;
  • 김정미 (경남대학교 식품영양학과) ;
  • 이종화 (안동대학교 식품생명공학과) ;
  • 김석주 (우송정보대학 식품영양조리과) ;
  • 전유진 (제주대학교 해양의생명과학부) ;
  • 박은주 (경남대학교 식품영양학과) ;
  • 이승철 (경남대학교 식품생명학과)
  • Received : 2010.12.15
  • Accepted : 2011.03.01
  • Published : 2011.03.31

Abstract

Styela plicata of large (9.82~11.66 g) and small (0.93~2.21 g) sizes harvested at different times was extracted with 4 different solvents (methanol, ethanol, acetone, and water). DPPH radical scavenging and reducing power was the highest in acetone extracts of small ones (38.98% and 1.724, respectively) harvested in November. The lowest radical scavenging activity was found in water extracts of large ones (12.03% and 0.114) in December. On the other hand, large ones harvested in September showed significantly higher inhibition rate of DNA damage (water, 56.54%; methanol, 55.83%; ethanol, 48.63%) than others. Overall, the water extraction of S. plicata tended to show a higher antigenotoxic effect. In addition, water extracts of large S. plicata from November showed the highest angiotensin converting enzyme (ACE) inhibitory activity (64.38%), while that of small ones from December exhibited the lowest (51.33%). Overall the results indicate that antioxidant and antihypertensive activities S. plicata are variable depending on harvesting time, size, and extraction solvent.

주름 미더덕(Styela plicata)을 8월부터 12월까지 매월 1회 채취하여 크기에 따라 큰 것(9.82~11.66 g)과 작은 것(0.93~2.21 g)으로 분류한 후 네 가지 용매(메탄올, 에탄올, 아세톤, 물)로 각각 추출 농축하여 추출물을 제조하였다. 각 추출물의 항산화 활성과 항고혈압 활성을 DPPH 라디칼 소거능과 환원력, 그리고 ACE 저해능으로 분석하였다. DPPH 라디칼 소거능은 11월에 채취된 큰 주름 미더덕의 아세톤 추출물이 가장 높았고, 환원력은 11월의 작은 미더덕의 아세톤 추출물이 가장 높았다. ACE 저해능은 물 추출물로만 측정하였는데, 11월에 채취된 큰 주름 미더덕의 추출물이 가장 높았다. 또한 주름 미더덕은 채취시기, 크기, 추출용매에 상관없이 거의 모든 시료에서 $H_2O_2$에 의해 유도된 백혈구의 DNA 손상을 억제하는 것으로 나타났으며 그 효과는 특히 8월 또는 9월에 채취한 큰 주름 미더덕이 월등함을 알 수 있었다. 또한 DNA 손상 억제에 있어서 추출 용매별 차이는 미미한 것으로 나타났으며 오히려 일부 물 추출물은 다른 용매 추출물에 비해 그 효과가 큰 것으로 나타나 끓이기 등의 일상 조리를 통한 주름 미더덕의 유효 성분 섭취를 기대할 수 있겠다. 이상의 결과로 채취시기, 크기, 용매에 따라 항산화, 항유전 독성 및 ACE 저해능의 차이가 있으며, 이는 주름 미더덕을 기능성 소재로 활용할 때 기초 자료로 활용될 수 있을 것이다.

Keywords

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