한국식품저장유통학회지 (Food Science and Preservation)

  • 제18권6호
  • /
  • Pages.859-868
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  • 2011
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
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현미코지를 이용한 쌀된장의 특성

Characteristics of Rice Doenjang Prepared with Brown Rice Koji

  • Lee, Seung-Eun (Department of Food Science and Technology, Chungju National University) ;
  • Suh, Hyung-Joo (Department of Food and Nutrition, Korea University) ;
  • Hwang, Jong-Hyun (Department of Food and Nutrition, Chungju National University)
  • 투고 : 2011.06.02
  • 심사 : 2011.10.28
  • 발행 : 2011.12.30
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초록

코지와 대두의 혼합비율을 달리하여 제조한 된장을 60일간 숙성하면서 품질을 비교 평가하였다. 대두와 현미코지의 비율은 1.5:1 (BR-1), 2:1 (BR-2), 3:1 (BR-3)로 하고, 대조구로써 대두와 백미코지를 2:1 (PR)로 하였다. 60일 숙성 후 수분과 염도는 시료 간에 큰 차이가 없었으나, 숙성 경과에 따라 pH, 산도 및 미생물수는 숙성 20~30일 째에 최대에 이르렀고. 이에 앞서 10~20일째에 환원당 함량과 에칠 알코올 함량이 최대로 증가하였으며 이러한 변화는 코지비율이 높을수록 빠르고 변화 값이 높은 경향이었다. 된장의 숙성지표가 되는 아미노태 질소 함량도 숙성 경과에 따라 증가하여 60일 숙성 후 BR-1이 가장 많은 887.6 mg%이었으며, 유리아미노산 함량도 BR-1이 가장 높아 $4047.0{\pm}1.5$ mg%이었으며, 코지비율이 높을수록 아미노산 생성이 많았다. GABA함량은 숙성 경과에 따라 소량 증가하는 것으로 나타났다. 색도는 숙성 경과에 따라 L값과 b값은 점차 감소하였으며 a값은 점차 증가하였다. 이와 같은 결과에서 현미코지를 이용한 된장은 백미코지를 이용한 된장에 비하여 숙성이 빠르게 진행되었고, 이와 함께 된장의 풍미를 결정하는 환원당, 에칠 알코올 및 아미노산의 함량이 상대적으로 다소 높게 나타났으며 이러한 변화는 숙성 30일 까지 뚜렷하게 나타남을 알 수 있었다. 이상의 결과에서 현미코지 된장은 백미코지 된장에 비하여 단백효소활성이 우수하므로 상대적으로 숙성이 빠르게 진행되며 에칠알코올과 아미노산 성분 등의 풍미물질이 많이 생성될 뿐만 아니라 현미의 영양성분이나 기능성물질인 GABA성분의 생성이 유도되어 산업적으로도 의미가 있는 것으로 생각되며, 현미코지 비율이 가장 높았던 BR-1이 품질면에서 바람직할 것으로 생각되었다.

This study was conducted to determine the appropriate mixing ratio of Koji and rice Doenjang. Brown rice Doenjang (BR) was prepared with three different ratios of cooked soybean to brown rice Koji [1.5:1 (BR-1); 2:1 (BR-2); and 3:1 (BR-3)], and polished-rice Doenjang (PR) was prepared with cooked soybean and polished-rice Koji (2:1). The components related to the quality of Doenjang were analyzed during 60-day aging. No significant differences were found in the moisture and salt contents between the brown rice Doenjang and the polished-rice Doenjang. The pH, titratable acidity, and bacteria cells increased at 20-30 days aging time, and decreased thereafter. The reducing-sugar and ethyl alcohol contents, on the other hand, increased at 10-20 days previously. These analytical values of the physicochemical and microbiological characteristics increased with the increase in the percentage of koji in each Doenjang. The amino-nitrogen and free-amino-acid contents showed the highest levels in BR-1: 887.6 and $4047.0{\pm}1.5$ mg%, respectively. The ${\gamma}$-aminobutyric-acid (GABA) contents slightly increased with increasing aging time. In the color of Doenjang, the lightness and yellowness increased along with the aging time, but the redness decreased as the aging time increased. These results showed that BR-1 processed the fermentation the fastest compared with the others. Moreover, the reducing-sugar, ethanol, and free-amino-acid contents as palatable components increased rapidly until 30 days aging time.

키워드

참고문헌

  1. Park JS (1992) Historical changes of Doenjang during the fermentation with different strains. Korean J Food Sci Technol, 24, 477-481
  2. Yoo JY (1998) Characteristics of meju and its microorganisms. Lecture 1. 1st symposium and expo for soybean fermentation foods, The Research Institute of Soybean Fermentation Food, Yeungnam Univ, Korea
  3. Song JY, Ahn CW, Kim JK (1984) Flavor components produced by microorganism during fermentation of korean ordinary soybean paste. Korean J Appl Microbiol Bioeng, 12, 147-152
  4. Song JY, Ahn CW, Kim JK (1984) Flavor components produced by microorganism during fermentation of Korean ordinary soybean paste. Korean J Appl Microbiol Bioeng, 12, 147-152
  5. Rhee CH, Kim WC, Rhee IK, Park HD (2008) Effects of inoculation of Bacillus subtilis cells on the fermentation of Korean traditional soy paste (Doenjang) Korean J Food Preserv, 15, 598-605
  6. Joo HK, Kim DH, Oh KT (1992) Chemical composition changes in fermented Doenjang depend on Koji and Its mixture. J Korean Agric Chem Soc, 35, 351-360
  7. Park KY, Hwang KM, Jung KO, Lee KB (2002) Studies on the standardization of Doenjang (Korean soybean paste). J Korean Soc Food Sci Nutr, 31, 343-350 https://doi.org/10.3746/jkfn.2002.31.2.343
  8. Choi KK, Choi CB, Ham SS, Lee DS (2003) Isoaltion, identification and growth characteristics of main strain related to Meju fermentation. J Korean Soc Food Sci Nutr, 32, 818-824 https://doi.org/10.3746/jkfn.2003.32.6.818
  9. Yoon IS, Kim HO, Youn SE, Lee KS (1977) Studies on the changes of N-Compounds during the fermentation process of the Korean Daenjang. Korean J Food Sci Technol, 9, 131-137
  10. Kim JK (2004) Changes of components affecting organoleptic quality during the ripening of traditional Korean soybean paste. J Fd Hyg Safety, 19, 31-37
  11. Kwon OJ, Kim JK, Chung YG (1986) The characteristics of isolated from ordinary Korean soy sauce and soybean paste. J Korean Agric Chem Soc, 29, 422-428
  12. Chang JK, Kim JK (1984) Statistical analysis for the relationship between gas chromatographic profiles of Korean ordinary soybean paste flavor and sensory evaluation. Korean J Appl Microbiol Bioeng, 12, 153-163
  13. Park JS, Lee MY, Kim KS, Lee TS (1994) Volatile flavor components of soybean paste (Doenjang) prepared from different types of strains. Korean J Food Sci Technol, 26, 255-260
  14. Choi KS, Rhee HS (1994) Characteristics of Doenjang made from different material and ratio of Koji. Korean J Soc Food Sci, 10, 39-44
  15. Lee KH, Cho SH (2003) Effect of the combined fermentation with Aspergillus oryzae and Bacillus natto on the quality improvement of Doenjang Meju. J Agric & Life Sci, 37, 9-21
  16. Chung SW, Kim YS, Chung KS (1995) Effects of preparation methods and aging temperature on the properties of rice-Doenjang. Agric Chem and Biotech, 38, 88-89
  17. Chung SW, Kwon DJ, Koo MS, Kim YS (1994) Quality characteristics and acceptance for Doenjang prepared with rice. Agric Chem and Biotech, 37, 266-271
  18. Kim JU, Choi JB, Bang CS (1989) Utilization of soymeal residue for rice Doenjang. J Korean Agric Chem Soc, 32, 98-103
  19. Sasagawa A, Gomi M, Ohura K, Yamazaki A, Yamada A (2005) Production of Miso based on Koji prepared from mixed different grains using high-pressure treatment. Nippon Shokuhin Kagaku Kaishi, 52, 485-490 https://doi.org/10.3136/nskkk.52.485
  20. Chou CC, Rwan JH (1995) Mycelial propagation and enzyme production in Koji prepared with Aspergillus oryzae on various rice extrudates and steamed rice. J of Ferm and Bioeng, 79, 509-512 https://doi.org/10.1016/0922-338X(95)91273-8
  21. Hwang JH, Lee SH, Choi HS (2010) Changes in enzyme activities of Koji prepared with molds from Meju. 2010 International Symposium and Annual Meeting of Korean Soc of Food Sci and Nutr, p 292
  22. Hwang JH, Choi HS, Lee SE, Kim SB, Lee SA, Kwak NK (2011) Changes of enzyme activities in rice Koji prepared with Aspergillus oryzae CM-4 by pretreatment conditions of raw materials. 2011 Annual Meeting of Korean Soc of Food Sci and Technol, p 315
  23. KFDA, Food code (2009)
  24. Chae SK, Kang KS, Ma SJ, Bang GW, Oh MH, Oh SH (2000) In standard food analysis, Gigu Munhwasa Co, Seoul, p 403-404
  25. Jo SJ, Hong CO, Yang SY, Choi KK, Kim HK, Yang HY, Lee KW (2011) Changes in contents of $\gamma$-aminobutyric acid (GABA) and isoflavones in traditional Korean Doenjang by ripening periods. J Korean Soc Food Sci Nutr, 40, 557-564 https://doi.org/10.3746/jkfn.2011.40.4.557
  26. Lee SK, Kim ND, Kim HJ, Park JS (2002) Development of traditional Doenjang improved in color. Korean J Food Sci Technol, 34, 400-406
  27. Chung DH, Sim SK (1994) Soybean Doenjang. in fermented soybean foods. Jisungjisaem Co, Seoul, Korea, p 632-652
  28. Yoon WJ, Lee SW, Moon HK, Moon JN, Kim BG, Kim BJ, Kim GY (2011) Quality characteristics of traditional soybean paste (Doenjang) manufactured with mixed beans. J East Asian Soc Dietary Life, 21, 375-384
  29. Nihon Miso Kijuts Kyokai (1997) The composition of Miso, Handbook of Miso Technology, p 59-74
  30. Cho JS (1980) Fermented soybean products. In survey on Korea fermented foods. Kijeon Pub Ltd, Chunju, Korea, p 47-90
  31. Kim EY, Rhyu MR (2000) The chemical properties of Doenjang prepared by Monascus koji. Korean J Food Sci Technol, 32, 1114-1121
  32. Lee SK, Kim ND, Kim HJ, Park JS (2002) Development of traditional Doenjang improved in color. Korean J Food Sci Technol, 34, 400-406
  33. Lee NS, Oh NS (1996) Characteristics of yeast flora and gas generation during fermentation of Doenjang. Agric Chem Biotechnol, 39, 255-259
  34. Shin SY, Kim YB, Yu TJ (1985) Flavour improvement of soybean pastes by the addition of Bacillus licheniformis and Saccharomyces rouxii. Korean J Food Sci Technol, 17, 18-14
  35. Kim MJ, Lee HS (1990) Studies on the changes of taste compounds during soy paste fermentation. Korean J Soc Food Sci, 6, 1-8
  36. Park SK, Seo KI, Choi SH, Moon JS, Lee, YH (2000) Quality assessment of commercial doenjang prepared by traditional method. J Korean Soc Food Sci Nutr, 29, 211-217
  37. Oh SH (2007) Effects and applications of germinated brown rice with enhanced levels of GABA. Food Sci Ind, 40, 41-46
  38. Lim SD, Kim KS (2009) Effects and utilization of GABA. Korean J Dairy Sci Technol, 27, 45-51
  39. Jo SJ, Hong CO, Yang SY, Choi KK, Kim HK, Yang H, Lee KW (2011) Korean traditional Doenjang, $\gamma$-aminobutyric acid(GABA), isoflavone. J Korean Soc Food Sci Nutr, 40, 557-564 https://doi.org/10.3746/jkfn.2011.40.4.557
  40. Hara A (1998) Preparative freshness of soybean paste. Food Sci, 40, 36-45
  41. Kim SS, Kim SK, Ryu MK, Cheigh HS (1983) Studies on the color improvement of Doenjang (fermented soybean paste) using various Aspergillus oryzae strains. Korean J Appl Microbiol Bioeng, 11, 67-74

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