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Antimutagenicity and Immuno Activity of Extracts from Epimedium koreanum Nakai Containing Different Icariin Quantity

Icariin 함량에 따른 삼지구엽초 추출물의 항돌연변이 및 면역활성

  • Park, Myoung-Su (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Seo-Jin (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Forghani, Fereidoun (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Rahman, S.M.E. (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Eo, Ji-Hyun (Dept. of Food Science and Technology and Functional Food Research Center Chonnam National University) ;
  • Eun, Jong-Bang (Dept. of Food Science and Technology and Functional Food Research Center Chonnam National University) ;
  • Oh, Deog-Hwan (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University)
  • 박명수 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 김서진 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 페레이둔 포가니 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 라만 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 어지현 (전남대학교 식품공학.기능성식품연구센터) ;
  • 은종방 (전남대학교 식품공학.기능성식품연구센터) ;
  • 오덕환 (강원대학교 바이오산업공학부 식품생명공학과)
  • Received : 2011.06.29
  • Accepted : 2011.12.02
  • Published : 2011.12.30

Abstract

Epimedium koreanum Nakai is a wild herb commonly consumed in South Korea due to its beneficial health effects. In the present study, the antimutagenic and immunoactivities of extracts from E. koreanum Nakai containing different icariin quantities were investigated for food use. In the Ames test, both the water and ethanol extracts were found not to have a mutagenic effect on Salmonella typhimurium TA98 and TA100, respectively. The E. koreanum Nakai extracts showed over 80 and 90% antimutagenic effects on benzo(${\alpha}$)pyrene (B(a)P) in S. typhimurium TA98 and TA100, respectively. Moreover, all the extracts showed over 70% antimutagenicity on S. typhimurium TA98 and TA100 against 4-nitroquinoline-1-oxide (4NQO). The E. koreanum Nakai extract with ethanol showed strong antimutagenic activity, higher than that of the water extract and the sequenced KE9412, KE9408, and KE9405. In the immunomodulating activity test, the effect of E. koreanum Nakai on the B (Rhamos) and T (Jukat) cells were investigated. The immunoactivity results showed that the growth and viability of the B and T cells increased and were activated more in KE9405 (1.8 times), KE9408 (1.6 times), and KE9412 (1.32 times) in the water extracts, and least in KE9412 (1.74 times), KE9408 (1.52 times), and KE9405 (1.4 times) in the ethanol extracts. In the case of both the water and ethanol extracts ($500{\mu}g/mL$) from E. koreanum Nakai, the highest cell number of the human B (Rhamos) and T (Jukat) cells was observed on day 4 in KE9405 and KE9412, and on day 5 in KE9408. Based on the obtained results, the development of E. koreanum Nakai as a food material is recommended.

예로부터 삼지구엽초는(Epimedium koreanum Nakai)는 한방에서 강장, 강정, 이뇨 등의 약재로 이용되어 왔다. 본 연구에서는, 삼지구엽초의 주성분인 icariin의 함량에 따른 항돌연변이 및 면역활성을 탐색하여 향후 기능성 식품소재로서 활용하고자 하였다. Ames test 결과, icariin 함량에 따른 삼지구엽초 추출물은 TA98 및 TA100 균주 모두에서 집락수가 음성대조군에 비하여 농도 변화에 따른 집락수의 큰 변화를 나타내지 않으므로 돌연변이원성이 없는 것으로 나타났다. 간접변이균인 B(a)P 항돌연변이 실험에서는 열수 및 에탄올 추출물이 각각 80% 그리고 90% 이상을 그리고, 직접변이균인 4NQO 항돌연변이균에서는 70% 이상으로 icariin 함량이 많은 순인 KE9412, KE9408, KE9405 순으로 항돌연변이 억제효과를 각각 나타내었다. 인간면역세포인 B와 T 세포를 이용한 면역활성 실험결과, 열수 추출물에서는 icariin 함량이 적은 KE9405가 1.8배로 가장 높은 활성을 나타냈으나, 에탄올 추출물에서는 열수 추출물과는 반대로 icariin 함량이 많은 KE9412가 1.74배로 가장 높은 활성을 나타내었다. 이러한 결과로 인하여, 삼지구엽초는 항돌연변이능과 면역활성 효능을 가지고 있으므로, 건강기능성 식품 소재로의 효용 가치가 높을 것으로 판단된다.

Keywords

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