Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Acute Toxicity Study on Insampaedok-san and Fermented Insampaedok-san

인삼패독산(人蔘敗毒散) 및 발효인삼패독산의 급성독성 연구

  • Im, Ga-Young (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Hwang, Yoon-Hwan (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Lee, Ji-Hye (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Oh, You-Chang (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Cho, Won-Kyung (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine)
  • 임가영 (한국한의학연구원 신한방제제연구센터) ;
  • 황윤환 (한국한의학연구원 신한방제제연구센터) ;
  • 이지혜 (한국한의학연구원 신한방제제연구센터) ;
  • 오유창 (한국한의학연구원 신한방제제연구센터) ;
  • 조원경 (한국한의학연구원 신한방제제연구센터) ;
  • 마진열 (한국한의학연구원 신한방제제연구센터)
  • Received : 2011.11.09
  • Accepted : 2011.12.16
  • Published : 2011.12.31
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Abstract

Objective : This study was carried out to investigate the acute toxicity and safety of Insampaedok-san and Fermented Insampaedok-san. Methods : SPF ICR male and female mice were administered orally with Insampaedok-san and Fermented Insampaedok-san. of 0(control group), 1,250, 2,500 and 5,000 mg/kg. After single administration, we daily examined number of deaths, clinical signs, gross findings and changes of body weight for 14 days. Hematological parameters and isolated organ weights were determined after 14 days of administration. Results : No dead animal and no significant changes of body weights were found during experimental period. In addition, no differences were found between control and all of treated groups in clinical signs, organ weights, hematology, and other findings. Conclusions : Insampaedok-san and Fermented Insampaedok-san. did not show any toxic effects and oral $LD_{50}$ values of the extracts was over 5,000 mg/kg in ICR mice.

Keywords

References

  1. 채육선. 중국의약회해. 서울 : 성보사. 1978 : 694-700.
  2. 보건복지가족부. 한의약 관련 법령집. 2009 : 206-207.
  3. Shim JY, Kim BW ; A study of effects of insampaedok-san and kamiinsampaedok san on the analgesi, antipyresis and thioacetamide induced liver damage in rats and mice. KH Univ O Med J. 1984 ; 7 : 323-334.
  4. 박영순. 한방의 약리해설. 서울 : 아카데미서적. 2002 : 1071.
  5. 이원익, 박병출, 김희성, 정기옥, 윤수인, 권순길, 김혜영. 단기간의 한약 복용후 독성간염과 함께 발생한 급성 간질성 신염 1예. 대한내과학회지. 2007 ; 73(1) : 103-106.
  6. 한국약학대학협의회 예방약학분과회, 독성학 -생명, 환경, 생태계-. 서울: 신일북스. 2008 : 251.
  7. 식품의약품안전청. 의약품등의 독성시험기준 식품의약품안전청고시, 2009-116, 2009.
  8. Organization for Economic Cooperation and Development(OECD). OECD Guideline for the Testing of Chemicals revised draft guideline 420. 2001.
  9. Wallace HW. Principles and methods of toxicology. New York : New York press. 1984 : 17-19.
  10. Choi JH, Hwang SJ, Jeong SN, Lee YK, Jin MH, Park SG, Lee CK ; Anti-aging Effect on Skin with 9 Repetitive Steaming and Fermenting Process Herbal Composition Extract. Kor. J. Herbology. 2009 ; 24(4) : 101-106.
  11. 주 굉. 상한류증활인서. 홍콩 : 상해운서루인행. 1907 : 14-18.
  12. 황도연. 의종손익. 서울 : 의약사. 1976 : 372-73.
  13. 문기홍. 제세보감. 서울: 행림서원. 1975 : 213.
  14. 서대춘, 서영태. 의서삼이종. 서울 : 경희대학교. 1974 : 1191.
  15. 오 겸. 의종금람. 서울 : 대성문화사. 1983 : 67-68.
  16. Eum HA, Lee JH, Kim DS, Chung TH, Lee YH, Um YR, Lee JH, Ma JY. Acute toxicity study on Insampaedok-san extracts in mice. Kor. J. Oriental Preventive Medical Society. 2010 ; 14(3) : 27-35.
  17. Cho SI, Kim HW, Lee GJ ; Biological activities of extracts fermented camellia japonica leaf and flower. Kor. J. Herbology. 2006 ; 21(2) : 55-62.
  18. Shin JY, Park SC, Kim KH, Bae CS, Kim SH, Shin DH, Kim JC. Acute toxicity evaluation of lemon grass(Cymbopogon citratus)Essential Oil in Rats. Lab Animal Research. 2005 ; 21(2) : 122-128.
  19. Ma JY, Huang DS, Seo CS, Lee SW, Kim JY, Shin HK. Acute toxicity study on Cheongsimyeonja-tang in mice. J of Sasang Constitutinal Medicine. 2010 ; 22(1) : 59-65.
  20. 이지혜, 엄영란, 심기석, 전원경, 이재훈, 마진열. 마우스를 이용한 발효쌍화탕의 급성 독성 실험. 대한한방내과학회지. 2009 ; 30(4) : 780-787.
  21. Ma JY, Huang DS, Lee NH, Ha HK, Yu YB, Shin HK. Acute toxicity study on Sipjeondaebo-tang in Rats. Kor. J. Oriental Physiology & Pathology. 2008 ; 22(5) : 1192-1195.
  22. 이재훈, 심기석, 박화용, 전원경, 엄영란, 마진열. 마우스에서 방풍통성산의 급성독성연구. 한방비만학회지. 2009 ; 9(1) : 71-78.