Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Comparison of the Antioxidant Effects of Diallyl Sulfide, Capsaicin, Gingerol and Sulforaphane in $H_2O_2$-Stressed HepG2 Cells

산화스트레스가 유도된 인체 간암세포 (HepG2)에서 Sulforaphane과 Diallyl Sulfide, Capsaicin, Gingerol의 항산화효과 비교연구

  • Lee, So-Youn (Department of Food and Nutrition and Research Institute of Obesity Sciences, Sungshin Women's University) ;
  • Wi, Hae-Ri (Department of Food and Nutrition and Research Institute of Obesity Sciences, Sungshin Women's University) ;
  • Lee, Myoung-Sook (Department of Food and Nutrition and Research Institute of Obesity Sciences, Sungshin Women's University)
  • 이소연 (성신여자대학교 식품영양학과, 비만과학연구소) ;
  • 위해리 (성신여자대학교 식품영양학과, 비만과학연구소) ;
  • 이명숙 (성신여자대학교 식품영양학과, 비만과학연구소)
  • Received : 2011.11.14
  • Accepted : 2011.12.13
  • Published : 2011.12.31
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Abstract

Oxygen is necessary to sustain life, yet cellular oxygen metabolism creates destructive elements called free radicals. Free radicals are chemically unbalanced and carrying free electrons that can damage molecules, potentially damaging the cell itself. For this reason, many antioxidant products, including supplements and functional foods, are being developed. In particular, natural products are rich sources of pharmacologically active compounds. The purpose of this study was to investigate the antioxidant effects of target biomaterials in Korean traditional spices such as diallyl sulfide (DAS), capsaicin (CAP), and gingerol (GGR), and to investigate the response of the antioxidant defense system to oxidative stress by hydrogen peroxide ($H_2O_2$) compared to sulforaphane (SFN) in HepG2 cells. After the analysis of the cell viability using Cell Counting kit-8 (CCK-8) assay, we determined that the optimum levels were $200{\mu}M$ DAS, $25{\mu}M$ CAP, $50{\mu}M$ GGR, and $12.5{\mu}M$ SFN. Antioxidant enzymes were measured and protein expression was detected by Western blotting. All treatments showed a significant decrease in antioxidant enzyme activity such as superoxide dismutase, catalse, and glutathione peroxidase in HepG2 cells. Additionally, DAS, CAP, GGR and SFN increased the antioxidant system-related transcription factor Nrf2 which was found to be regulated by the activation of MAPK-JNK in this study. In conclusion, these results indicate the protective effects of DAS CAP, GGR, and SFN against $H_2O_2$-induced oxidative stress.

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References

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