Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Antioxidant Effects of the Moxi with Ginger Tar Produced by Moxibustion with Ginger Combustion

격강구의 연소과정에서 생성된 격강구진의 항산화 효과

  • Seo, Sang-Rok (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Won-Kwang University) ;
  • Yang, Seung-Bum (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Won-Kwang University) ;
  • Kim, Jae-Hyo (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Won-Kwang University) ;
  • Ahn, Seong-Hun (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Won-Kwang University) ;
  • Sohn, In-Chul (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Won-Kwang University)
  • 서상록 (원광대학교 한의과대학 경혈학교실) ;
  • 양승범 (원광대학교 한의과대학 경혈학교실) ;
  • 김재효 (원광대학교 한의과대학 경혈학교실) ;
  • 안성훈 (원광대학교 한의과대학 경혈학교실) ;
  • 손인철 (원광대학교 한의과대학 경혈학교실)
  • Received : 2011.11.07
  • Accepted : 2011.12.15
  • Published : 2011.12.27
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Abstract

Objectives : This study estimated antioxidant effects of the moxi with ginger tar (MGT) ; chemical combustion products produced by moxibustion with ginger during combustion. Methods : To do this aim, we investigated total polyphenol and flavonoid contents, SOD (superoxide dismutase) scavenging activity, ABTS (2.2-azino-bis-3-erthylbenzo-thiazoline-6- sulfonic acid) & DPPH(2,2-Dipheny1-1 -picryl-hydrazyl) radical's scavenging ability of MGT. Results : Total polyphenol contents of MGT was $7.8{\pm}0.09$ mg/g in 10 mg/ml, SOD activity was $42.51{\pm}3.39%$ in 200 ug/ml, DPPH radical scavenging effect of MGT was $83.24{\pm}0.01%$ in 200 ug/ml and ABTS radical scavenging effect was $41.88{\pm}0.16%$ in 200 ug/ml. Conclusions : In this study, the effects of moxi with ginger could be induced by not only heating stimulus on acupoints but also chemical stimulus produced during combustion of moxibustion, MGF. The advanced study about biological mechanism through meridian system, skin aging, and inflammation on MGT will be required.

Keywords

References

  1. 손인철. 알기쉬운 뜸치료법. 서울 : 의성당. 2009 : 47-8.
  2. 우현수, 이윤호, 김창환. 灸法에 대한 文獻的 考察 및 최근 研究 動向. 대한침구학회지. 2002 ; 19(4) : 1-15.
  3. 西谷郁子(李红捷译). 灸及艾的燃烧生成物局部涂敷治愈疣赘2例报告. 国外医学(中医中樂分冊) 1988 ; 10(6) : 49.
  4. 西谷郁子, 植田伸夫. 艾の燃烧生成物に含まれる抗酸化作用物资にっいて. 全日本灸学會雜志. 1988 ; 38(1) : 29.
  5. 안성훈, 구성태, 도진우, 김종성, 김광수, 양범식 외. 구진(Moxi tar)이 면역세포에서 iNSO합성에 미치는 영향. 대한결락경혈학회지. 2000 ; 17(1) : 33-46.
  6. 안영남, 안성훈, 양기중, 김풍식, 김광수, 김종성 외. 구진(Moxi tar)이 뇌신경쇠포에서 Nitirc Oxide 생성에 미치는 영향. 대한경혈학회지. 2000 ; 17(2) : 25-44.
  7. 唐照亮, 章复清, 宋小鸽, 侯正明, 陈全珠, 袁静. 艾灸對實驗性炎症大鼠血清SOD 活性和MDA 含量的影響. 安徽中医学院学報 1998 ; 17(5) : 45-7.
  8. 안성훈, 안영남, 백대봉, 송문영, 김경식, 손인철. 구진의 $H_{2}O_{2}$로 유발된 뇌신경세포 상해에 대한 보호기전 연구. 경락경혈학회지. 2005 ; 22(2) : 43-56.
  9. Ahotupa M, Saxelin M, Korpela R. Antioxidative properties of Lactobacillus GG. Nutr Today. 1996 ; 31(Suppl.) : 51S-52S.
  10. Valko M, Leibfriz D, Moncol J, Cronin MT, Mazur M, Teler J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007 ; 39 : 44-8. https://doi.org/10.1016/j.biocel.2006.07.001
  11. 서근영, 이성원, 박성주, 김성철, 손인철, 황성연, 안성훈. 염산 가수분해 추출법으로 조제된 자하거 약침액의 생리활성 효과. 대한약침학회지. 2010 ; 13(2) : 5-12.
  12. Folin O, W. Dennis. On phosphotungasticphosohomdybdic com-pounds as color reagents, J. Biol. Chem. 1912 ; 12(2) : 239-49.
  13. Marklund S, Marklund G. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 1974 ; 47(3) : 469-74. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  14. Dietz BM, Kang YH, Liu G, Eggler AL, Yao P, Chadwick LR, Pauli GF, Famsworth NR, Mesecar AD, van Breemen RB, and Bolton JL. Xanthohumol isolated from Humulus Iupulus inhibits menadione-induced DNA damage through induction of quinone reductase. Chem Res Toxicol. 2005 ; 18 : 1296-305. https://doi.org/10.1021/tx050058x
  15. Van den Berg R, Haenen GR. Van den Berg H, Bast A. Applicability of an improved Trolox equivalent anti-oxidant capacity(TEAC) assay for evaluation of anti-oxidant capacity measurements of mixture. Food Chem. 1999 ; 66 : 511-7. https://doi.org/10.1016/S0308-8146(99)00089-8
  16. Zhu DPQ : The Role of Moxibustion in Traditional Chinese Acupuncture. American Journal of Acupuncture. 1984 ; 12(2) : 125-32.
  17. 조명래, 이재동. 박동석 외. 藥物 種類에 따른 間接灸의 燃燒特性에 關한 硏究. 대한침구학회지. 1999 ; 16(4) : 345-63.
  18. 김용석, 隔薑灸의 燃燒特性에 關한 硏究. 慶熙大學校 大學院. 학위논문. 1997.
  19. 全国韓医科大学 本草学共同敎材编集委員會 編著. 本草学. 서울 : 永林社. 2004 : 447-8.
  20. 張馬合註. 黃帝內經素問. 서울 : 成輔社. 1975 : 44, 84, 87.
  21. 李梴. 國譯編註醫學入門. 崇文社(重刊). 1974 : 885.
  22. 彈玉堂. 傷寒論鍼灸配穴選注. 서울 : 일중사. 1992 : 100-2.
  23. 卞赫. 반복적 隔薑灸 시술이 深部 열전달에 미치는 영향. 東國大學校大學院. 학위논문. 2011.
  24. 임성택, 김경호, 김갑성. 애구의 물리적 특성에 관한 연구. 대한침구학회지. 1994 ; 11(1) : 327-36.
  25. 박영배, 강성길, 허웅. 애구의 연소 특성에 관한 실험적 연구 : 애주의 밀도를 중심으로. 경희대학교 한의과대학 논문집. 1994 ; 17(1) : 191-9.
  26. 박영배, 강성길, 김창환, 고형균, 오환섭, 허웅. 재료에 따른 뜸의 연소특성에 관한 연구. 대한한의학회지. 1996 ; 17(1) : 222-33.
  27. 최용태, 이윤호, 박동석, 안병철, 최도영, 이재동, 남상수, 강기원. 애구의 연소 구간별 열량비에 대한 연구. 대한침구학회지. 1998 ; 15(2) : 173-81.
  28. 김윤홍, 이승호, 여수정, 최일환, 김영곤, 임사비나. 구법의 표준화를 위한 온도 측정 연구. 대한침구학회지. 2008 ; 25(2) : 129-37.
  29. 千永實, 金昌煥, 姜成吉, 高炯均, 朴英培, 金容奭. 艾絨의 標準化 作業에 대한 硏究動向. 대한침구학회지. 1997 ; 14(1) : 55-69.
  30. 김재효, 이관형, 안영남, 김용득, 김경식, 손인철. 애엽 및 구진 약침이 일과성 전뇌 허혈 손상에 미치는 효과. 대한경락경혈학회지. 2003 ; 20(3) : 61-80.
  31. Bauer G. Reactive oxygen and nitrogen species: Efficient, selective signals during intercellular induction of apoptosis. Anticancer Res. 2000 ; 20 : 4115-39.
  32. Branen AL. Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc. 1975 ; 52 : 59-65. https://doi.org/10.1007/BF02901825
  33. Madsen HL, Nielsen BR, Bertelsen G, Skibsted LH. Screen of antioxidative activity of spices. Food Chem. 1996 ; 57 : 331-77. https://doi.org/10.1016/0308-8146(95)00248-0
  34. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. polyphenolic compound content and antioxidative activities by extract from seed, sprout and flower of safflower. J. Korean Soc. Food Sic. Nutr. 2000 ; 29 : 1127-32.
  35. Kuramoto T. Development and application of food materials from extract such as SOD. Up-to data Food Process. 1992 ; 27 : 22-3.
  36. Bondent V, Brand-Williams W, Bereset C. Kinetics and mechanism of antioxidant activity using the DPPH free radical methods. Lebensm Wiss Technol. 1997 ; 30 : 609-15.
  37. Yoo KM, Kim DO, Lee CY. Evaluation of different methods of antioxidant measurement. Food Sci Biotechnol. 2007 ; 16 : 177-82.
  38. NJ. Miller, C Rice-Evans, MJ. Davies, V. Copinaththan and A. Milner. A novel method for measuring anti-oxidant capacity and its application to monitoring the antioxidant status in premature neonants. Clicical Science. 1993 ; 26 : 265-77.
  39. Branen AL. Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc. 1975 ; 52 : 59-65. https://doi.org/10.1007/BF02901825